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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (02): 344-351.doi: 10.3724/SP.J.1006.2012.00344


Effect of Continuous Aeration on Growth and Activity of Enzymes Related to Nitrogen Metabolism of Different Rice Genotypes at Tillering Stage

ZHAO Feng1,2,ZHANG Wei-Jian1,ZHANG Xiu-Fu2,*,WANG Dan-Ying2,XU Chun-Mei2   

  1. 1 Institute of Applied Ecology, Nanjing Agricultural University, Nanjing 210095, China; 2 State Key Laboratory of Rice Biology / China National Rice Research Institute, Hangzhou 310006, China; 3 Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064,China
  • Received:2011-05-31 Revised:2011-10-12 Online:2012-02-12 Published:2011-12-06
  • Contact: 章秀福, E-mail: zhangxf169@ sohu.com, Tel: 0571-63370584

Abstract: In order to clarify the role of oxygen in the growth and development of rice and its mechanism of nitrogen, three rice genotypes, i.e., “Guodao 1” (indica), “Xiushui 09” (japonica), and “Brazilian upland rice” (upland rice), were cultured in nutrient solution with continuous aeration to measure the growth-related parameters and the activity of enzymes related to nitrogen metabolism at tillering stage. The results showed that, compared with the control, lower chlorophyll content in leaves was found in all the genotypes. The dry weights of shoots and roots were decreased by 44% and 40% for “Guodao 1” under continuous aeration, respectively, whereas no significant effects were observed for the “Brazilian upland rice” and “Xiushui 09”. The root length, specific surface area, and the intensity of α-naphthylamine oxidation of roots were increased significantly by continuous aeration, irrespective of the genotypes. The nitrogen accumulation in the “Guodao 1” and “Brazilian upland rice” under aeration was reduced by 35.8% and 36%, respectively. Therefore, the nitrate reductase activity (NRA) in leaves was significantly increased, while the glutamine synthetase activity (GSA) was decreased under aeration. The NRA in leaves of “Xiushui 09” under continuous aeration tended to increase (P > 0.05) in comparison to the control. Thus, continuous aeration could increase the surface area and oxidation intensity in rice roots, and reduce the chlorophyll content and GSA in rice leaves, which may reduce nitrogen absorption and dry matter accumulation. In addition, these were significant differences in responses of rice genotypes to continuous aeration.

Key words: Rice, Continuous aeration, Genotypes, Root development, Nitrogen metabolism

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