Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (02): 352-359.doi: 10.3724/SP.J.1006.2012.00352

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of PASP-KT-NAA on Photosynthesis and Antioxidant Enzyme Activities of Maize Seedlings under Low Temperature Stress

XU Tian-Jun1,DONG Zhi-Qiang1,*,LAN Hong-Liang1,PEI Zhi-Chao1, 2,GAO Jiao1,XIE Zhen-Xing1   

  1. 1 Institute of Crop Science, Chinese Academy of Agricultural Sciences/ Key Laboratory of Crop Eco-physiology and Cultivation, Beijing 100081, China; 2 Beijing Agricultural Technology Extension Station, Beijing 100029, China
  • Received:2011-03-31 Revised:2011-07-25 Online:2012-02-12 Published:2011-11-07
  • Contact: 董志强, E-mail: dongzhiqiang@126.com, Tel: 010-82106043

PDF

1507

Cited

17

Abstract: A pet experiment of maize seedlings was conducted to investigate the effects of low temperature stress and the chemical regulator of PASP-KT-NAA (PKN) on photosynthetic, physiological parameters, chlorophyll contents, and various antioxidant enzymes activities. The results showed that, in maize seedlings under low temperature stress the photosynthesis ability and SOD, POD, CAT activities reduced; the formation speed of hydrogen peroxide and superoxide anion, as well as MDA contents increased. PKN pretreatment increased Pn, Gs, Fv/Fm, and chlorophyll contents. Under low temperature stress for seven days, Pn, Gs, Fv/Fm, chlorophyll contents of ZDTR and FDTR were higher than those of CK, which was increased by 88.95% and 61.11%, 593.33% and 1 741.67%, 111.50% and 145.16%, 36.61% and 54.03%. While the formation speed of hydrogen peroxide and superoxide anion reduced, MDA contents decreased too. Simultaneously, SOD, POD, CAT activities were increased by 292.59% and 632.98%, 295.07% and 360.54%, 254.55% and 265.45%. In conclusion, PKN pretreatment can improve the cold resistance of maize seedlings

Key words: Low temperature stress, Antioxidant enzyme, Photosynthesis, Maize seedlings, PKN regulation

[1]Meng Y(孟英), Li M(李明), Wang L-M(王连敏), Wang L-Z(王立志), Feng Y-J(冯延江). Effects of chilling injury on maize and correlative research. Heilongjiang Agric Sci (黑龙江农业科学), 2009, (4): 150-153 (in Chinese with English abstract)
[2]Ma Q-S(马树庆), Xi Z-X(袭祝香), Wang Q(王琪). Risk evaluation of cold damage to corn in northeast China. J Nat Disasters (自然灾害学报), 2003, 12(3):137-141 (in Chinese with English abstract)
[3]Guan X-J(关贤交), Ou-Yang X-R(欧阳西荣). The research of low temperature on maize. Crop Res (作物研究), 2004, (1): 16 (in Chinese with English abstract)
[4]He J(何洁), Liu H-X(刘鸿先), Wang Y-R(王以柔), Guo J-Y(郭俊彦). Low temperature and photosynthesis of plants. Plant Physiol Commun (植物生理学通讯), 1986, 22(2): 1-6 (in Chinese with English abstract)
[5]Shi Z-Z(史占忠), Ben X-M(贲显明), Zhang J-T(张敬涛). The emerging pattern and preventive measure of maize cold damage in the sanjiang river plain. Heilongjiang Agric Sci (黑龙江农业科学), 2003, (2): 7-10 (in Chinese with English abstract)
[6]Fryer M J, Andrews J R, Oxborough K. Relationship between CO2 assimilation, photosynthetic electron transport, and active O2 metabolism in leaves of maize in the field during periods of low temperature. Plant Physiol, 1998, 116: 571-580
[7]Gao M-Z(高妙真). Mechanism of chilling injury of corn and the effect of chemical control to prevent chilling. J Northeast Agric Univ (东北农业大学学报), 1989, 20(4): 295-299 (in Chinese with English abstract)
[8]Kraus T E, Fletcher R A Paclobutrazol protects wheat seedlings from heat and paraquat injury: is detoxification of active oxygen involved. Plant Cell Physiol, 1994, 35: 45-52
[9]Beaucorps G D, Leymomnie J P. Potassium and Crop Resistance to the Frost (钾与作物抗霜冻). Nanjing: Phoenix Science Press, 1989. pp 29-37
[10]Zhang F-S(张福锁). Environment Stress and Plant Nutrition (环境胁迫与植物营养). Beijing: China Agricultural University Press, 1993. pp 148-170
[11]Zou G-Y(邹国元), Yang Z-F(杨志福), Li X-L(李晓林). Effect of potassium on chilling resistance of maize seedling. Plant Nutr Fert Sci (植物营养与肥料学报), 1998, 4(2): 165-169 (in Chinese with English abstract)
[12]Liu Y(刘芸), Zhong Z-C(钟章成), Marinus J A, Cao G-X(操国兴), Yin K-L(尹克林), Long Y(龙云). Effects of α-NAA and UV-B radiation on photosynthetic pigments and activities of protective enzymes in Trichosanthes kirilowii Maxim leaves. Acta Eco Sin (生态学报), 2003, 23(1): 12-15 (in Chinese with English abstract)
[13]Leshem Y Y, Wurzburger, Wurzburger J, Grossman S. Cytokinin interaction with tree radical metabolism and senescence. Physiol Plants, 1981, 53: 9-12
[14]Leng Y-X(冷一欣), Rei X-S(芮新生), He P-H(何佩华). Study of the yield increasing of maize by using polyaspartic acid. J Maize Sci (玉米科学), 2005, 13(3): 100-102 (in Chinese with English abstract)
[15]Arnon D I, Whatley F R. Factors influencing oxygen production by illuminated chloroplast fragments. Archiv Biochem, 1949, 23: 141-156
[16]Lin Z-F(林植芳), Li S-S(李双顺), Lin G-Z(林桂珠), Guo J-Y(郭俊彦). The accumulation of hydrogen peroxide in senescing leaves and chloroplasts in relation to lipid peroxidation. Acta Photophysiol Sin (植物生理学报), 1988, 14(1): 16-22 (in Chinese with English abstract)
[17]Wang R-G(王爱国), Luo G-H(罗广华). Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants. Plant Physiology Commun (植物生理学通讯), 1990, 6: 55-57 (in Chinese with English abstract)
[18]Chance B, Maehly A C. Assay of catalase and peroxidase. Methods Enzymol, 1955, 12: 764-775
[19]Li Z-G(李忠光), Li H-J(李江鸿), Du C-K(杜朝昆). Simultaneous measurement of five antioxidant enzyme activities using a single extraction system. J Yunnan Normal Univ (Nat Sci Edn) (云南师范大学学报•自然科学版), 2002, 6(10): 44-48 (in Chinese with English abstract)
[20]Lin Z-F(林植芳), Li S-S(李双顺), Lin G-Z(林桂珠), Sun G-C(孙谷畴), Guo J-Y(郭俊彦). Superoxide dismutase activity and lipid peroxidation in relation to senescence of rice leaves. J Integr Plant Biol (植物学报), 1984, 26(6): 605-615 (in Chinese with English abstract)
[21]Demming-Adams B. Carotenoids and photoprotection in plants: a role of Xanthophyll zeaxanthin. Biochim Biophys Acta, 1987, 1020: 1-24
[22]Li Y-M(李月梅), Ma Y-Y(马莹莹), Yang L-L(杨英良), Meng L(孟良). The effect of low temperature on photosynthesis andrespiration. Heilongjiang Agric Sci (黑龙江省农业科学), 1991, 6(1): 4-8 (in Chinese)
[23]Fan H-F(樊怀福), Jiang W-J(蒋卫杰), Guo S-R(郭世荣). Effects of low temperature on growth and photosynthesis of tomato seedlings. Jiangsu Agric Sci (江苏农业科学), 2005, 3(7): 89-91 (in Chinese with English abstract)
[24]Allen D J, Ort D R. Impacts of chilling temperatures on photosynthesis in warm climate plant. Trends Plant Sci, 2001, 6: 36-41
[25]Wu X-X(吴雪霞), Chen J-L(陈建林), Zha L-S(查丁石). Effects of low temperature stress on photosynthetic characteristics in leaves of egg plant seedlings. Acta Agric Boreali-Sin (华北农学报), 2008, 23(5): 185-189 (in Chinese with English abstract)
[26]Meng F-J(孟繁静), Liu D-H(刘道宏), Su Y-Y(苏业瑜). Plant Physiology and Biochemical (植物生理生化). Beijing: China Agriculture Press, 1995. pp 11-16 (in Chinese)
[27]Wang L-M(王连敏), Wang L-Z(王立志). Effect of low temperature at seedling stage on proline, electric conductivity and photosynthesis. Chin J Agrometeorol (中国农业气象), 1999, 20(2): 28-31 (in Chinese with English abstract)
[28]Jiang L(江力), Kong X-W(孔小卫), Zhang R-X(张荣铣). Effects of 6-benzyladenine and abscisic acid on the photosynthetic function decline in tobacco. J Nanjing Agric Univ (南京农业大学学报), 2006, 29(4): 127-130 (in Chinese with English abstract)
[29]Chen J-M(陈建明), Yu X-P(俞晓平), Cheng J-A(程家安). The application of chlorophyll fluorescence kinetics in the study of physiological responses of plants to environmental stresses. Acta Agric Zhejiangensis (浙江农业学报), 2006, 18(1): 51-55 (in Chinese with English abstract)
[30]Mauro S, Dainsese P, Lannoye R, Bassi R. Cold resistant and cold susceptible maize lines differ in the phosphorylation of the photo system II subunit. Plant Physiol, 1997, 115: 171-177
[31]Powles S B, Berry J A, Björkman O. Interaction between light and temperature on the inhibition of photosynthesis in chilling-sensitive plants. Plant Cell Environ, 1983, 6: 117-123
[32]Carrasco R M, Rodriguez J S, Perez P. Changes in chlorophyll fluorescence during the course of photoperiod and in response to drought in Casuarina Equisetifolia Forst. Photosynthetica, 2002, 40: 363-368
[33]Liu Q-D(刘清岱), Zhu Y-R(朱晔荣), Tao H-L(陶汉林), Wang Y(王勇). The change of PSII of Spirod elapolyrrhiz a half-2-fronds and the effect of cytokinin. In: Abstracts of Research Papers Presented at the 9th National Meeting of the Chinese Society for Plant Physiology. Shanghai: Biophysical Chemistry, 2004
[34]Zhang R(张蕊), Lü J(吕俊), Mi Q-S(米青山), Wang S-Y(王三根). Effects of salicylic acid on the antioxidant enzymes of rice seedlings during chilling stress. J Southwest Agric Univ (Nat Sci) (西南农业大学学报•自然科学版), 2006, 28(1): 29-36 (in Chinese with English abstract)
[35]Zhang J-X(张敬贤), Li J-M(李俊明), Zhang H-M(张海明). The effect of low temperature on activities of cell protective enzymes and protoplasmic parameters in leaves of Zea mays. Acta Agric Boreali-Sin (华北农学报), 1993, 8(3): 9-12 (in Chinese with English abstract)
[36]Xiong D-J(熊冬金), Lin Z-H(林志红), Chang B-Y(畅柏云), Zhu B-F(朱必凤), Zhu Y-L(朱友林). Studies of four kind of isozymes and the changes of content of mailond laldehyde in maiiein waterlogging or chilling stress. J Nanchang Univ (南昌大学学报), 1996, 20(4): 314-319 (in Chinese with English abstract)
[37]Back K H, Skinner D Z. Alteration of antioxidant enzyme gene expression during cold ac climation of near-isogonics wheat lines. Plant Sci, 2003, 165: 1221-1227
[38]Liao X-R(廖祥儒), He P-C(贺普超), Zhu X-C(朱新产). Effect of zeatbsf in H2O2 scavenging system of Vitis vulpina leaf disks under salt stress. Acta Bot Sin (植物学报), 1997, 39(7): 641-646 (in Chinese with English abstract)
[39]Yin K-D(殷奎德), Hu W-Y(胡文玉). Cytokinin effect on active oxygen during in vitro senescence of wheat leaves. J Heilongjiang Bayi Agric Univ (黑龙江八一农垦大学学报), 1994, 7(4): 16-22 (in Chinese with English abstract)
[1] SONG Shi-Qin, YANG Qing-Long, WANG Dan, LYU Yan-Jie, XU Wen-Hua, WEI Wen-Wen, LIU Xiao-Dan, YAO Fan-Yun, CAO Yu-Jun, WANG Yong-Jun, WANG Li-Chun. Relationship between seed morphology, storage substance and chilling tolerance during germination of dominant maize hybrids in Northeast China [J]. Acta Agronomica Sinica, 2022, 48(3): 726-738.
[2] GAO Zhen, LIANG Xiao-Gui, ZHANG Li, ZHAO Xue, DU Xiong, CUI Yan-Hong, ZHOU Shun-Li. Effects of irrigating at different growth stages on kernel number of spring maize in the North China Plain [J]. Acta Agronomica Sinica, 2021, 47(7): 1324-1331.
[3] LI Zeng-Qiang, DING Xin-Chao, LU Hai, HU Ya-Li, YUE Jiao, HUANG Zhen, MO Liang-Yu, CHEN Li, CHEN Tao, CHEN Peng. Physiological characteristics and DNA methylation analysis under lead stress in kenaf (Hibiscus cannabinus L.) [J]. Acta Agronomica Sinica, 2021, 47(6): 1031-1042.
[4] ZHOU Lian, LIU Chao-Xian, XIONG Yu-Han, ZHOU Jing, CAI Yi-Lin. Functional analysis of plasma membrane intrinsic protein ZmPIP1;1 involved in drought tolerance and photosynthesis in maize [J]. Acta Agronomica Sinica, 2021, 47(3): 472-480.
[5] HOU Hui-Zhi, ZHANG Xu-Cheng, FANG Yan-Jie, YU Xian-Feng, WANG Hong-Li, MA Yi-Fan, ZHANG Guo-Ping, LEI Kang-Ning. Effects of micro ridge-furrow with plastic mulching on soil hydrothermal environment and photosynthesis at seedling stage of spring wheat on cold rain-fed area [J]. Acta Agronomica Sinica, 2020, 46(9): 1398-1407.
[6] Jing-Nan ZOU,Qi YU,Xi-Jun JIN,Ming-Yao WANG,Bin QIN,Chun-Yuan REN,Meng-Xue WANG,Yu-Xian ZHANG. Effects of exogenous melatonin on physiology and yield of soybean during seed filling stage under drought stress [J]. Acta Agronomica Sinica, 2020, 46(5): 745-758.
[7] TIAN Wen-Gang,ZHU Xue-Feng,SONG Wen,CHENG Wen-Han,XUE Fei,ZHU Hua-Guo. Ectopic expression of S-adenosylmethionine decarboxylase (GhSAMDC1) in cotton enhances salt tolerance in Arabidopsis thaliana [J]. Acta Agronomica Sinica, 2019, 45(7): 1017-1028.
[8] YU Qi,FENG Nai-Jie,WANG Shi-Ya,ZUO Guan-Qiang,ZHENG Dian-Feng. Effects of S3307 on the photosynthesis and yield of mung bean at R1 and R5 stages under waterlogging stress [J]. Acta Agronomica Sinica, 2019, 45(7): 1080-1089.
[9] YAN Qing-Qing,ZHANG Ju-Song,DAI Jian-Min,DOU Qiao-Qiao. Effects of glycinebetain on photosynthesis and biomass accumulation of island cotton seedlings under saline alkali stress [J]. Acta Agronomica Sinica, 2019, 45(7): 1128-1135.
[10] Han-Yu WU,Fei XIAO,Ya-Li ZHANG,Chuang-Dao JIANG,Wang-Feng ZHANG. Repetitive intense flashes inhibit photosystem II activity and thermal dissipation in cotton leaves [J]. Acta Agronomica Sinica, 2019, 45(5): 792-797.
[11] Xiao-Han MA,Jie ZHANG,Huan-Wei ZHANG,Biao CHEN,Xin-Yi WEN,Zi-Cheng XU. Exogenous MeJA improves cold tolerance of tobacco by inhibiting H2O2 accumulation [J]. Acta Agronomica Sinica, 2019, 45(3): 411-418.
[12] Ning HE,Xue-Yang WANG,Liang-Zi CAO,Da-Wei CAO,Yu LUO,Lian-Zi JIANG,Ying MENG,Chun-Xu LENG,Xiao-Dong TANG,Yi-Dan LI,Shu-Ming WAN,Huan LU,Xu-Zhen CHENG. Effects of photoperiods and temperatures on physiological characteristics and chlorophyll synthesis precursors of adzuki bean seedlings [J]. Acta Agronomica Sinica, 2019, 45(3): 460-468.
[13] Han-Yu WU,Ya-Jun ZHANG,Wang-Feng ZHANG,Ke-Ru WANG,Shao-Kun LI,Chuang-Dao JIANG. Photosynthetic characteristics of senescent leaf induced by high planting density of maize at heading stage in the field [J]. Acta Agronomica Sinica, 2019, 45(2): 248-255.
[14] Qing-Hua YANG,Bo-Yuan ZHENG,Lei-Lei LI,Shuang-Jie JIA,Xin-Pei HAN,Jia-Meng GUO,Yong-Chao WANG,Rui-Xin SHAO. Effect of Exogenous Nitric Oxide Donor on Carbon Assimilation and Antioxidant System in Leaves of Maize Seedlings under PEG-induced Water Deficit Stress [J]. Acta Agronomica Sinica, 2018, 44(9): 1393-1399.
[15] Li-Li WAN, Zhuan-Rong WANG, Qiang XIN, Fa-Ming DONG, Deng-Feng HONG, Guang-Sheng YANG. Enhanced Accumulation of BnA7HSP70 Molecular Chaperone Binding Protein Improves Tolerance to Drought Stress in Transgenic Brassica napus [J]. Acta Agronomica Sinica, 2018, 44(04): 483-492.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No.12 Zhongguancun South Street, Beijing 100081,China Email:xbzw@chinajournal.net.cn
Supported by Beijing Magtech Co.Ltd