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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (02): 360-368.doi: 10.3724/SP.J.1006.2012.00333

• RESEARCH NOTES • Previous Articles     Next Articles

Cloning and Expression Analysis of GmMYB Induced by Abiotic Stresses

SUN Xia1,**,LIU Jin-Yue1,**,YUAN Xiao-Hui1,PAN Xiang-Wen1,DU Wei-Guang2,REN Hai-Xiang2,MA Yong-Bo3,Jun ABE 4,QIU Li-Juan5,*,LIU Bao-Hui1,*   

  1. 1 Laboratory of Soybean Molecular Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences / Key Laboratory of Mollisols Agroecology, Chinese Academy of Sciences, Harbin 150081, China; 2 Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences, Mudanjiang 157041, China; 3 Liaoning Agricultural Environmental Protection Monitoring Station, Shenyang 110034, China; 4 Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan; 5 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2011-06-21 Revised:2011-10-12 Online:2012-02-12 Published:2011-12-01
  • Contact: 刘宝辉, E-mail:liubh@neigaehrb.ac.cn; 邱丽娟, E-mail:qiu_lijuan@263.net

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Abstract: Response to external environment is the outcome of stress-induced gene expression. In this paper, based on one stress-induced EST sequence, we cloned four R2R3-MYB genes from soybean cultivar Dongnong 42, whose genomic sequences consisted of three exons and two introns. Three of them corresponding to Gm02g1300, Gm03g38040, and Gm10g01340 are respectively consistent with the sequences of Willams 82. A mutation at the 375th single nucleotide in the sequence of Gm19g40650 from Dongnong 42 caused a synonymous amino acid substitution (E125–D125). To test the relationship of four MYB genes with stress resistance, we treated the seedlings of cultivar Dongnong 42 with abiotic stresses including salt, alkali, drought and low temperature in the artificial climate chamber. Quantitative PCR analysis indicated that all of the four genes were transient down-regulated or up-regulated when subjected to the stresses, but different in the expression time, level and tendency. Gm02g01300 was induced by drought stress while Gm03g38040 was strongly induced by multiple stresses, indicating that they play important roles in responding to external stresses. There were also differences in the expression of individual gene between cotyledons and embryos. These results under a variety of abiotic stress conditions suggest that the four R2R3-MYB genes are different not only in the expression patterns, but also in the regulation modes.

Key words: Abiotic stress, GmMYB, Bud period, Expression analysis

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