Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (02): 344-351.doi: 10.3724/SP.J.1006.2012.00344

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effect of Continuous Aeration on Growth and Activity of Enzymes Related to Nitrogen Metabolism of Different Rice Genotypes at Tillering Stage

ZHAO Feng1,2,ZHANG Wei-Jian1,ZHANG Xiu-Fu2,*,WANG Dan-Ying2,XU Chun-Mei2   

  1. 1 Institute of Applied Ecology, Nanjing Agricultural University, Nanjing 210095, China; 2 State Key Laboratory of Rice Biology / China National Rice Research Institute, Hangzhou 310006, China; 3 Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064,China
  • Received:2011-05-31 Revised:2011-10-12 Online:2012-02-12 Published:2011-12-06
  • Contact: 章秀福, E-mail: zhangxf169@ sohu.com, Tel: 0571-63370584

PDF

1508

Cited

10

Abstract: In order to clarify the role of oxygen in the growth and development of rice and its mechanism of nitrogen, three rice genotypes, i.e., “Guodao 1” (indica), “Xiushui 09” (japonica), and “Brazilian upland rice” (upland rice), were cultured in nutrient solution with continuous aeration to measure the growth-related parameters and the activity of enzymes related to nitrogen metabolism at tillering stage. The results showed that, compared with the control, lower chlorophyll content in leaves was found in all the genotypes. The dry weights of shoots and roots were decreased by 44% and 40% for “Guodao 1” under continuous aeration, respectively, whereas no significant effects were observed for the “Brazilian upland rice” and “Xiushui 09”. The root length, specific surface area, and the intensity of α-naphthylamine oxidation of roots were increased significantly by continuous aeration, irrespective of the genotypes. The nitrogen accumulation in the “Guodao 1” and “Brazilian upland rice” under aeration was reduced by 35.8% and 36%, respectively. Therefore, the nitrate reductase activity (NRA) in leaves was significantly increased, while the glutamine synthetase activity (GSA) was decreased under aeration. The NRA in leaves of “Xiushui 09” under continuous aeration tended to increase (P > 0.05) in comparison to the control. Thus, continuous aeration could increase the surface area and oxidation intensity in rice roots, and reduce the chlorophyll content and GSA in rice leaves, which may reduce nitrogen absorption and dry matter accumulation. In addition, these were significant differences in responses of rice genotypes to continuous aeration.

Key words: Rice, Continuous aeration, Genotypes, Root development, Nitrogen metabolism

[1]Fan J B, Zhang Y L, Turner D, Duan Y H, Wang D S, Sheng Q R. Root physiological and morphological characteristics of two rice cultivars with different nitrogen-use efficiency. Pedosphere, 2010, 20: 446–455
[2]Amara W, Hank G, Campbell J T. The use of agar nutrient solution to simulate lack of convection in waterlogged soils. Ann Bot, 1996, 80: 115–123
[3]Kirk G J D. Rice root properties for internal aeration and efficient nutrient acquisition in submerged soil. New Phytol, 2003, 159: 185–194
[4]Wang X-L(汪晓丽), Feng K(封克), Sheng H-J(盛海君), Chen P(陈平). Kinetics of nitrate uptake by different rice genotypes and the effects of ammonium on nitrate uptake at the seedling stage. Sci Agric Sin (中国农业科学), 2003, 36(11): 1306–1310 (in Chinese with English abstract)
[5]Jampeetong A, Brix H. Oxygen stress in Salvinia natans: interactive effects of oxygen availability and nitrogen source. Environ Exp Bot, 2009, 66: 153–159
[6]Wang X B, Wu P, Hu B, Cheng Q S. Effects of nitrate on the growth of lateral root and nitrogen absorption in rice. Acta Bot Sin, 2002, 44: 678–683
[7]Wang X Z, Zhu J G, Gao R, Yasukazu H, Feng K. Nitrogen cycling and losses under rice-wheat rotations with coated urea and urea in the Taihu lake region. Pedosphere, 2007, 17: 62–69
[8]Zou C-Q(邹春琴), Fan X-Y(范晓云), Shi R-L(石荣丽), Zhang F-S(张福锁). Effect of ammonium and nitrate nitrogen on the growth and iron nutrition of up- and lowland rice. J China Agric Univ (中国农业大学学报), 2007, 12(14): 45–49 (in Chinese with English abstract)
[9]Kant S, Kafkafi U. Ammonium and nitrate as a nitrogen source for plants. Adv Plant Physiol, 2003, 5: 463–478
[10]Duan Y H, Zhang Y L, Shen Q R, Wang S W. Nitrate effect on rice growth and nitrogen absorption and assimilation at different growth stages. Pedosphere, 2006, 16: 707–717
[11]Kronzucker H J, Siddiqi M Y, Glass A D M, Kirk G J D. Nitrate ammonium synergism in rice: a subcellular flux analysis. Plant Physiol, 1991: 1041–1045
[12]Yoshida S, Forno D A, Cock J H, Gomez K A. Laboratory Manual for Physiological Studies of Rice. Manila, Philippines: IRRI, 2006
[13]Mu Z X, Zhang S Q, Zhang L S, Liang A H, Liang Z S. Hydraulic conductivity of whole root system is better than hydraulic conductivity of single root in correlation with the leaf water status of maize. Bot Stud, 2006, 47: 145–151
[14]Tachibana S, Konishi N. Diurnal variation of in vivo and in vitro nitrate reductase activity in cucumber plants. J Jpn Soc Hort Sci, 1991, 60: 593–599
[15]Zhao S-J(赵世杰), Shi G-A(史国安), Dong X-C(董新纯). Laboratory Guide for Plant Physiology (植物生理学实验指导). Beijing: China Agricultural Science and Technology Press, 2002. p 47 (in Chinese)
[16]Akira Y. The Root System: a dynamic Dynamic Integration of Components Differing in Morphology and Function. Proceeding of the 6th Symposium of the International Society of Root Research. Nagoya, Japan, November 11–15, 2001. pp 2–3
[17]Armstrong J, Armstrong W. Rice: sulphide-induced barriers to root radial oxygen loss, Fe2+ and water uptake, and lateral root emergence. Ann Bot, 2005, 96: 625–638
[18]Malik A I, Colmer T D, Lambers H, Schortemeyer M. Aerenchyma formation and radial O2 loss along adventitious roots of wheat with only the apical root portion exposed to O2 deficiency. Plant Cell Environ, 2003, 26: 1713–1722
[19]Mcdonald M P, Galwey N W, Colmer T D. Similarity and diversity in adventitious root anatomy as related to root aeration among a range of wetland and dryland grass species. Plant Cell Environ, 2002, 25: 441–451
[20]Pezeshki S R, Delaune R D. Responses of Spartina alterniflora and Spartina patens to rhizosphere oxygen deficiency. Acta Oecol, 1996, 17: 365–378
[21]Deng D(邓丹), Wu K-W(吴可为), Deng H(邓泓). Effects of zone oxygenation on growth and Cd accumulation in paddy rice (Oryza sativa L.). Acta Ecol Sin (生态学报), 2009, 29(5): 2520–2526 (in Chinese with English abstract)
[22]Colmer T D. Aerenchyma and an inducible barrier to radial oxygen loss facilitate root aeration in upland, paddy and deep-water rice (Oryza sativa L.). Ann Bot, 2003, 91: 301–309
[23]Vartapetian B B. Plant anaerobic stress as a novel trend in ecological physiology, biochemistry and molecular biology: 2. Further development of the problem. Russian J Plant Physiol, 2007, 53: 711–738
[24]Delaune R D, Pezeshki S R, Pardue J H. Anoxidation-reduction buffer for evaluating physiological response of plants to root oxygen stress. Environ Exp Bot, 2005, 30: 243–247
[25]Wang D-Y(王丹英), Han B(韩勃), Zhang X-F(章秀福). Effect of oxygen content in rice rhizosphere on growth of the roots. Acta Agron Sin (作物学报), 2008, 34(5): 803–808 (in Chinese with English abstract)
[26]Liesack W, Schnell S, Revsbech N P. Microbiology of flooded rice paddies. FEMS Microbiol Rev, 2000, 24: 625–645
[27]Zhao F(赵锋), Xu C-M(徐春梅), Zhang W-J(张卫建), Zhang X-F(章秀福), Cheng J-P(程建平), Wang D-Y(王丹英). Effect of rhizosphere dissolution oxygen and nitrogen form on root traits and N accumulation. Chin J Rice Sci, 2011, 25(3): 195–200 (in Chinese with English abstract)
[28]Zhu L-F(朱练峰), Liu X(刘学), Yu S-M(禹盛苗), Ou-Yang Y-N(欧阳由男), Jin Q-Y(金千瑜). Effect of aerated irrigation on physiological characteristics and senescence at late growth stage of rice. Chin J Rice Sci (中国水稻科学), 2010, 24(3): 257–263 (in Chinese with English abstract)
[29]Zhao F(赵锋), Wang D-Y(王丹英), Xu C-M(徐春梅), Zhang W-J(张卫建), Li F-B(李凤博), Mao H-J(毛海军), Zhang X-F(章秀福). Response of morphological, physiological and yield characteristics of rice (Oryza sativa L.) to different oxygen-increasing patterns in rhizosphere. Acta Agron Sin (作物学报), 2010, 36(2): 303–312 (in Chinese with English abstract)
[30]Lee K W, Chen P W, Lu C A, Chen S, David Ho T H, Yu S M. Coordinated responses to oxygen and sugar deficiency allow rice seedlings to tolerate flooding. Sci Signal, 2009, 2(91): ra61
[31]Ponnampernma F N. The chemistry of submerged soils. Adv Agron,1972, 24: 29–96
[32]Colmer T D, Cox C H, Voesenek L A. Root aeration in rice (Oryza sativa): Evaluation of oxygen ,carbon dioxide, and ethylene as possible regulations of root acclimatizations. New Phytol, 2006, 170: 767–778
[33]Kirk G J D. Rice root properties for internal aeration and efficient nutrient acquisition in submerged soil. New Phytol, 2003, 159: 185–194
[34]Li Y(李勇), Zhou Y(周毅), Guo S-W(郭世伟), Shen Q-R(沈其荣). Effects of different N forms on root morphology and water absorption of lowland and upland rice plants. Chin J Rice Sci (中国水稻科学), 2007, 21(3): 294–298 (in Chinese with English abstract)
[35]Wallsgrove R M, Turner J C, Hall N P, Kendall A C, Bright S W J. Barley mutants lacking chloroplast glutamine synthetase biochemical and genetic analysis. Plant Physiol, 1987, 83: 155–158
[36]Robinson J M, Baysdorfer C. Inter-relationship between photosynthetic carbon and nitrogen metabolism in mature soybean leaves and isolated leaf mesophyll cells. In: Health R L, Preiss J, eds. Carbon Partitioning in Photosynthetic Tissue. American Society of Plant Physiology, Rockville, MD. 1985. pp. 333–357
[37]Rufty T W, Huber S C, Volk R J. Alternations in leaf carbohydrate metabolism in response to nitrogen stress. Plant Physiol, 1988, 88: 725–730
[1] TIAN Tian, CHEN Li-Juan, HE Hua-Qin. Identification of rice blast resistance candidate genes based on integrating Meta-QTL and RNA-seq analysis [J]. Acta Agronomica Sinica, 2022, 48(6): 1372-1388.
[2] ZHENG Chong-Ke, ZHOU Guan-Hua, NIU Shu-Lin, HE Ya-Nan, SUN wei, XIE Xian-Zhi. Phenotypic characterization and gene mapping of an early senescence leaf H5(esl-H5) mutant in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2022, 48(6): 1389-1400.
[3] ZHOU Wen-Qi, QIANG Xiao-Xia, WANG Sen, JIANG Jing-Wen, WEI Wan-Rong. Mechanism of drought and salt tolerance of OsLPL2/PIR gene in rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1401-1415.
[4] ZHENG Xiao-Long, ZHOU Jing-Qing, BAI Yang, SHAO Ya-Fang, ZHANG Lin-Ping, HU Pei-Song, WEI Xiang-Jin. Difference and molecular mechanism of soluble sugar metabolism and quality of different rice panicle in japonica rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1425-1436.
[5] YAN Jia-Qian, GU Yi-Biao, XUE Zhang-Yi, ZHOU Tian-Yang, GE Qian-Qian, ZHANG Hao, LIU Li-Jun, WANG Zhi-Qin, GU Jun-Fei, YANG Jian-Chang, ZHOU Zhen-Ling, XU Da-Yong. Different responses of rice cultivars to salt stress and the underlying mechanisms [J]. Acta Agronomica Sinica, 2022, 48(6): 1463-1475.
[6] YANG Jian-Chang, LI Chao-Qing, JIANG Yi. Contents and compositions of amino acids in rice grains and their regulation: a review [J]. Acta Agronomica Sinica, 2022, 48(5): 1037-1050.
[7] DENG Zhao, JIANG Nan, FU Chen-Jian, YAN Tian-Zhe, FU Xing-Xue, HU Xiao-Chun, QIN Peng, LIU Shan-Shan, WANG Kai, YANG Yuan-Zhu. Analysis of blast resistance genes in Longliangyou and Jingliangyou hybrid rice varieties [J]. Acta Agronomica Sinica, 2022, 48(5): 1071-1080.
[8] YANG De-Wei, WANG Xun, ZHENG Xing-Xing, XIANG Xin-Quan, CUI Hai-Tao, LI Sheng-Ping, TANG Ding-Zhong. Functional studies of rice blast resistance related gene OsSAMS1 [J]. Acta Agronomica Sinica, 2022, 48(5): 1119-1128.
[9] ZHU Zheng, WANG Tian-Xing-Zi, CHEN Yue, LIU Yu-Qing, YAN Gao-Wei, XU Shan, MA Jin-Jiao, DOU Shi-Juan, LI Li-Yun, LIU Guo-Zhen. Rice transcription factor WRKY68 plays a positive role in Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2022, 48(5): 1129-1140.
[10] WANG Xiao-Lei, LI Wei-Xing, OU-YANG Lin-Juan, XU Jie, CHEN Xiao-Rong, BIAN Jian-Min, HU Li-Fang, PENG Xiao-Song, HE Xiao-Peng, FU Jun-Ru, ZHOU Da-Hu, HE Hao-Hua, SUN Xiao-Tang, ZHU Chang-Lan. QTL mapping for plant architecture in rice based on chromosome segment substitution lines [J]. Acta Agronomica Sinica, 2022, 48(5): 1141-1151.
[11] WANG Ze, ZHOU Qin-Yang, LIU Cong, MU Yue, GUO Wei, DING Yan-Feng, NINOMIYA Seishi. Estimation and evaluation of paddy rice canopy characteristics based on images from UAV and ground camera [J]. Acta Agronomica Sinica, 2022, 48(5): 1248-1261.
[12] KE Jian, CHEN Ting-Ting, WU Zhou, ZHU Tie-Zhong, SUN Jie, HE Hai-Bing, YOU Cui-Cui, ZHU De-Quan, WU Li-Quan. Suitable varieties and high-yielding population characteristics of late season rice in the northern margin area of double-cropping rice along the Yangtze River [J]. Acta Agronomica Sinica, 2022, 48(4): 1005-1016.
[13] CHEN Yue, SUN Ming-Zhe, JIA Bo-Wei, LENG Yue, SUN Xiao-Li. Research progress regarding the function and mechanism of rice AP2/ERF transcription factor in stress response [J]. Acta Agronomica Sinica, 2022, 48(4): 781-790.
[14] WANG Lyu, CUI Yue-Zhen, WU Yu-Hong, HAO Xing-Shun, ZHANG Chun-Hui, WANG Jun-Yi, LIU Yi-Xin, LI Xiao-Gang, QIN Yu-Hang. Effects of rice stalks mulching combined with green manure (Astragalus smicus L.) incorporated into soil and reducing nitrogen fertilizer rate on rice yield and soil fertility [J]. Acta Agronomica Sinica, 2022, 48(4): 952-961.
[15] QIN Qin, TAO You-Feng, HUANG Bang-Chao, LI Hui, GAO Yun-Tian, ZHONG Xiao-Yuan, ZHOU Zhong-Lin, ZHU Li, LEI Xiao-Long, FENG Sheng-Qiang, WANG Xu, REN Wan-Jun. Characteristics of panicle stem growth and flowering period of the parents of hybrid rice in machine-transplanted seed production [J]. Acta Agronomica Sinica, 2022, 48(4): 988-1004.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd