Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (02): 333-343.doi: 10.3724/SP.J.1006.2012.00333

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effect of Wheat/Maize/Soybean and Wheat/Maize/Sweet Potato Relay Strip Intercropping on Bacterial Community Diversity of Rhizosphere Soil and Nitrogen Uptake of Crops

YONG Tai-Wen,YANG Wen-Yu*,XIANG Da-Bing,ZHU Zhen-Ying   

  1. Agronomy College, Sichuan Agricultural University, Wenjiang 611130, China
  • Received:2011-04-08 Revised:2011-09-11 Online:2012-02-12 Published:2011-12-01
  • Contact: 杨文钰, E-mail: wenyu.yang@263.net

PDF

1044

Cited

4

Abstract: The aim of this study was to understand the relationship between diversity of rhizosphere bacterial community and nitrogen uptake of crops in two relay strip intercropping systems: wheat/maize/soybean and wheat/maize/sweet potato. We analyzed the diversities of rhizosphere bacterial community in five cropping systems using denaturing gradient gel electrophoresis (DGGE) based on 16S rDNA. The cropping systems included wheat-soybean (A1), wheat-sweet potato (A2), maize single cropping (A3), wheat/maize/soybean (A4), and wheat/maize/sweet potato (A5). Compared to the sole cropping systems (A1, A2, and A3 treatments), the A4 treatment showed increases in grain nitrogen uptake and total nitrogen uptake amounts of aboveground of crops at both flowering (or silking) and maturity stages, and the Shannon-Weiner indices for rhizosphere bacterial community diversity was also increased significantly. The values of nitrogen uptake and the Shannon-weiner index in the relay strip intercropping systems were greater than those in sole cropping systems, and the values in the treatment with soybean stubble were greater than those in the treatment with sweet potato stubble. The number and strength of the DNA bands from DGGE profiles varied with the cropping systems, especially for the distinguished bands. The comparability of rhizosphere bacterial community composition was low among different cropping systems. The similarity coefficient (Cs) was higher between two relay strip intercropping systems than between relay strip intercropping system and sole cropping system, but the Cs value was low between A4 and A5 treatments. However, there was obvious difference of rhizosphere bacterial community composition between A4 and A5 treatments, and the A4 treatment was propitious to enhancing rhizosphere bacterial community diversity and increasing nitrogen uptakes of crops.

Key words: Wheat/maize/soybean relay strip intercropping, Nitrogen uptake, Bacterial community diversity, PCR-DGGE

[1]Li L, Sun J H, Zhang F S, Li X L, Rengel Z, Yang S C. wheat/maize or wheat/soybean strip intercropping: I. Yield advantage and interspecific interactions on nutrients. Field Crops Res, 2001, 71: 123–137
[2]Eaglesham A R J, Ayanaba A, Rao V R. Improving the nitrogen nutrition of maize by intercropping with cowpea. Soil Biol Biochem, 1981, 13: 169–171
[3]Gianfrda L, Sannino F, Violante A. Pesticide effects on the activity of free immobilized and soil invertase. Soil Biol Biochem, 1995, 27: 1201–1208
[4]Li X-Y(李秀英), Zhao B-Q(赵秉强), Li X-H(李絮花), Li Y-T(李燕婷), Sun R-L(孙瑞莲), Zhu L-S(朱鲁生), Xu J(徐晶), Wang L-X(王丽霞), Li X-P(李小平), Zhang F-D(张夫道). Effects of different fertilization systems on soil microbe and its relation to soil fertility. Sci Agric Sin (中国农业科学), 2005, 38(8): 1591–1599 (in Chinese with English abstract)
[5]Yong T-W(雍太文), Yang W-Y(杨文钰), Ren W-J(任万军), Fan G-Q(樊高琼), Xiang D-B(向达兵). Analysis of the nitrogen transfer, nitrogen uptake and utilization in the two relay-planting systems. Sci Agric Sin (中国农业科学), 2009, 42(9): 3170–3178 (in Chinese with English abstract)
[6]Yong T-W(雍太文), Yang W-Y(杨文钰), Fan G-Q(樊高琼), Wang X-C(王小春), Zhang Y-F(张亚飞). Study on the annual balance application of nitrogen fertilizer in the relay-cropping system of “wheat/maize/soybean”. Soil & Fert Sci China (中国土壤与肥料), 2009, (3): 31–35 (in Chinese with English abstract)
[7]Li L(李隆), Yang S-C(杨思存), Sun J-H(孙建好), Li X-L(李晓林), Zhang F-S(张福锁). Interspecific competition and facilitation in wheat/soybean intercropping system. Chin J Appl Ecol (应用生态学报), 1999, 10(2): 197–200 (in Chinese with English abstract)
[8]Li W X, Li L, Sun J H, Guo T W, Zhang F S, Bao X G, Peng A, Tang C. Effects of intercropping and nitrogen application on nitrate present in the profile of an Orthic Anthrosol in Northwest China. Agric Ecosyst & Environ, 2005, 105: 483–49
[9]Ye Y-L(叶优良),Li L (李隆), Suo D-R(索东让). Effect of wheat/maize and faba bean/maize intercropping on soil nitrate nitrogen concentration and accumulation. Ecol & Environ (生态环境), 2008, 17(1): 377–383 (in Chinese with English abstract)
[10]Wang Y-F(王艳芳). Progress of research on soil nitrogen transfer and movement. J Ningxia Agric Coll (宁夏农学院学报), 2004, 25(1): 53-56 (in Chinese with English abstract)
[11]Hatfield J L, Stewart B A. Soil Biology: Effects on Soil Quality. Boca Raton, USA: Lewis Publishers, 1994
[12]Sugden A M. Ecology: diversity and ecosystem resilience. Science, 2000, 290: 233–235
[13]Walter K D, Margaret K B, Courtney S C. Biological properties of soil and subsurface sediments under abandoned pasture and cropland. Soil Biol Biochem, 1997, 28: 837–846
[14]Song Y-N(宋亚娜), Marschner P, Zhang F-S(张福锁), Bao X-G(包兴国), Li L(李隆). Effect of intercropping on bacterial community composition in rhizoshpere of wheat (Triticum aestivum L.), maize (Zea mays L.) and faba bean (Vicia faba L.). Acta Ecol Sin (生态学报), 2006, 26(7): 2268–2274 (in Chinese with English abstract)
[15]Zhang J-E(章家恩), Gao A-X(高爱霞), Xu H-Q(徐华勤), Luo M-Z(罗明珠). Effects of maize/peanut intercropping on rhizosphere soil microbes and nutrient contents. Chin J Appl Ecol (应用生态学报), 2009, 20(7): 1597–1602 (in Chinese with English abstract)
[16]Xie K-Z(解开治), Xu P-Z(徐培智), Li K-H(李康活), Zhou S-C(周少川), Chen J-S(陈建生), Tang S-H(唐拴虎), Zhang F-B(张发宝), Huang X(黄旭). Effects of three different cropping system on diversity of soil bacterial community. Plant Nutr Fert Sci (植物营养与肥料学报), 2009, 15(5): 1107–1113 (in Chinese with English abstract)
[17]Gu Y-F(辜运富), Zhang X-P(张小平), Tu S-H(涂仕华), Sun X-F(孙锡发), Kristina L. Effect of long-term fertilization on nitrification and nitrobacteria community in a purple paddy soil under rice-wheat rotations. Acta Ecol Sin (生态学报), 2008, 28(5): 2123–2130 (in Chinese with English abstract)
[18]Chen Z(陈哲), Yuan H-Z(袁红朝), Wu J-S(吴金水), Wei W-X(魏文学). Activity and composition of the denitrifying bacterial community respond differently to long-term fertilization. Acta Ecol Sin (生态学报), 2009, 29(11): 5923–5929 (in Chinese with English abstract)
[19]Li Y(李杨), Cen J(岑剑), Wang B-L(王保莉), Qu D(曲东). RFLP analysis on the impact of microbial diversity of dry land soils without nitrogenous fertilizer. Agric Res Arid Areas (干旱地区农业研究), 2009, 27(2): 199–203 (in Chinese with English abstract)
[20]Luo H F, Qi H Y, Zhang H X. Assessment of the bacterial diversity in fenvalerate-treated soil. World J Microbiol Biotechnol, 2004, 20: 509–515
[21]Xiao Y B, Li L, Zhang F S. Effect of root contact on interspecific competition and N transfer between wheat and faba bean using direct and indirect 15N techniques. Plant & Soil, 2004, 262: 45–54
[22]Chu G-X(褚贵新), Shen Q-R(沈其荣), Li Y-L(李奕林), Zhang J(张娟), Wang S-Q(王树起). Researches on Bi-directional N transfer between the intercropping system of groundnut with rice cultivated in aerobic soil using 15N foliar labelling method. Acta Ecol Sin (生态学报), 2004, 24(2): 278–284 (in Chinese with English abstract)
[23]Li S-M(李少明), Zhao P(赵平), Fan M-P(范茂攀), Gao S-C(高世昌), Zheng Y(郑毅). Nitrogen uptake and utilization in intercropping system of maize and soybean. J Yunnan Agric Univ (云南农业大学学报), 2004, 19(5): 527–574 (in Chinese with English abstract)
[24]Xiao Y-B(肖焱波), Li L(李隆), Zhang F-S(张福锁). An outlook of the complementary nitrogen nutrition in the legume// graminaceae System. Rev China Agric Sci & Technol (中国农业科技导报), 2003, 5(6): 44–49 (in Chinese with English abstract)
[25]Abbott L K, Murphy D V. Soil Biological Fertility. Netherlands: Kluwer Academic Publishers, 2003
[26]Wang Y(王瑛), Meng Y-L(孟亚利), Chen B-L(陈兵林), Zhou Z-G(周治国), Shu H-M(束红梅), Bian H-Y(卞海云). Studies on the soil microorganism quality and soil nutrient content at the rhizosphere and non-rhizosphere region of cotton in wheat-cotton intercropping system. Acta Ecol Sin (生态学报), 2006, 26(10): 3485–3490 (in Chinese with English abstract)
[27]Dong Y(董艳), Tang L(汤利), Zheng Y(郑毅), Zhu Y-Y(朱有勇), Zhang F-S(张福锁). Effects of nitrogen application rate oil rhizosphere microbial community in wheat-faba bean intercropping system. Chin J Appl Ecol (应用生态学报), 2008, 19(7): 1559–1566 (in Chinese with English abstract)
[28]Yong T-W(雍太文), Chen X-R(陈小容), Yang W-Y(杨文钰), Xiang D-B(向达兵), Fan G-Q(樊高琼). Effect of different cropping systems on root exudates and above ground nitrogen uptake and soil nutrition of wheat. Acta Agron Sin (作物学报), 2010, 36(3): 477–485 (in Chinese with English abstract)
[1] KE Jian, CHEN Ting-Ting, XU Hao-Cong, ZHU Tie-Zhong, WU Han, HE Hai-Bing, YOU Cui-Cui, ZHU De-Quan, WU Li-Quan. Effects of different application methods of controlled-release nitrogen fertilizer on grain yield and nitrogen utilization of indica-japonica hybrid rice in pot-seedling mechanically transplanted [J]. Acta Agronomica Sinica, 2021, 47(7): 1372-1382.
[2] TIAN Chang, JIN Tuo, ZHOU Xuan, HUANG Si-Yi, WANG Ying-Zi, XU Ze, PENG Jian-Wei, RONG Xiang-Min, XIE Gui-Xian. Effects of controlled-release urea on nitrogen uptake characteristics and yield of double-cropping rice around Dongting Lake area [J]. Acta Agronomica Sinica, 2021, 47(4): 691-700.
[3] LIAO Ping,LIU Lei,HE Yu-Xuan,TANG Gang,ZHANG Jun,ZENG Yong-Jun,WU Zi-Ming,HUANG Shan. Interactive effects of liming and straw incorporation on yield and nitrogen uptake in a double rice cropping system [J]. Acta Agronomica Sinica, 2020, 46(01): 84-92.
[4] Tian-Yi YU,Xiao-Liang LI,Ya LU,Xue-Wu SUN,Yong-Mei ZHENG,Zheng-Feng WU,Pu SHEN,Cai-Bin WANG. Effect of phosphorus (P) on nitrogen (N) uptake and utilization in peanut [J]. Acta Agronomica Sinica, 2019, 45(6): 912-921.
[5] Dan-Dan HE,Li-Guo JIA,Yong-Lin QIN,Ming-Shou FAN. Classification of potato cultivars by their nitrogen use efficiency [J]. Acta Agronomica Sinica, 2019, 45(1): 153-159.
[6] LI Xiao-Feng,CHENG Jin-Qiu,LIANG Jian,CHEN Meng-Yun,REN Hong-Ru,ZHANG Hong-Cheng,HUO Zhong-Yang*,DAI Qi-Gen,XU Ke,WEI Hai-Yan,GUO Bao-Wei. Effects of Total Straw Returning and Nitrogen Application on Grain Yield and Nitrogen Absorption and Utilization of Machine Transplanted Japonica Rice [J]. Acta Agron Sin, 2017, 43(06): 912-924.
[7] GUO Bao-Wei,ZHANG Hong-Cheng*,ZHU Da-Wei,Xu Ke,HUO Zhong-Yang,WEI Hai-Yan,DAI Qi-Gen, HU Ya-Jie,CUI Pei-Yuan. Characteristics of Nitrogen Uptake, Utilization and Distribution in Ordered Transplanting and Optimized Broadcasting Rice [J]. Acta Agron Sin, 2017, 43(01): 97-111.
[8] ZHOU Lan,YANG Yong,WANG Zhan-Hai,CHEN Fu,ZENG Zhao-Hai. Influence of Maize-soybean Rotation and N fertilizer on Bacterial Community Composition [J]. Acta Agron Sin, 2013, 39(11): 2016-2022.
[9] HUO Zhong-Yang,LI Jie,ZHANG Hong-Cheng,DAI Qi-Gen,XU Ke,WEI Hai-Yan,GONG Jin-Long. Characterization of Nitrogen Uptake and Utilization in Rice under Different Planting Methods [J]. Acta Agron Sin, 2012, 38(10): 1908-1919.
[10] CAO Lan-Qin,WU Xiao-Ming,YANG Rui,TIAN Yang-Yang,CHEN Xian-Ni,CHEN Bi-Yun,LI Ya-Jun,GAO Ya-Jun. Differences of Nitrogen Status between Different N-uptake-efficiency Rapeseed (Brassica napus L.) Cultivars [J]. Acta Agron Sin, 2012, 38(05): 887-895.
[11] YONG Tai-Wen,CHEN Xiao-Rong,YANG Wen-Yu*,XIANG Da-Bing,FAN Gao-Qiong. Root Exudates and Nitrogen Uptake of Wheat in wheat/Maize/Soybean relay Cropping system [J]. Acta Agron Sin, 2010, 36(3): 477-485.
[12] DONG Gui-Chun,YU Xiao-Feng,ZHAO Jiang-Nin,JU Jing,TIAN Hao,LI Jin-Qian,ZHANG Yan. General Characteristics of Nitrogen Uptake and Utilization in Conventional Indica Rice Cultivars with Different Panicle Weight Types [J]. Acta Agron Sin, 2009, 35(11): 2091-2100.
[13] ZHANG Yong-Li;YU Zhen-Wen. Effects of Irrigation Amount on Nitrogen Uptake, Distribution, Use, and Grain Yield and Quality in Wheat [J]. Acta Agron Sin, 2008, 34(05): 870-878.
[14] SHI Yu;YU Zhen-Wen;WANG Dong;LI Yan-Qi;WANG Xue. Effects of Nitrogen Rate and Ratio of Base Fertilizer and Topdressing on Uptake, Translocation of Nitrogen and Yield in Wheat [J]. Acta Agron Sin, 2006, 32(12): 1860-1866.
[15] XUE Xiao-Ping ;WANG Yi-Lin;GUO Wen-Qi;CHEN Bing-Lin;ZHOU Zhi-Guo. Mathematical Model of Quantitative Diagnosis on Dynamic Nitrogen Demand of Cotton after Anthesis Based on Critical Nitrogen Concentration Diluation Model [J]. Acta Agron Sin, 2006, 32(10): 1579-1585.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd