Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (04): 596-605.doi: 10.3724/SP.J.1006.2012.00596

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

DNA Fingerprinting of Sesame (Sesamum indicum L.) Varieties (Lines) from Recent National Regional Trials in China

LIU Hong-Yan,WU Kun,YANG Min-Min,ZUO Yang,ZHAO Ying-Zhong*   

  1. Key Laboratory of Oil Crops Biology and genetic breeding of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Science, Wuhan 430062, China
  • Received:2011-08-17 Revised:2011-12-19 Online:2012-04-12 Published:2012-02-13
  • Contact: 赵应忠, E-mail: yingzhzh@public.wh.hb.cn

PDF

1336

Cited

3

Abstract: DNA fingerprinting is an important technique for plant variety identification and protection. In this study, 43 sesame (Sesamum indicum L.) varieties (lines) from recent National Regional Trail were amplificated with 12 pairs of SSR primers, and a total of 30 polymorphic bands were obtained. These molecular markers were further used for variety (lines) distinction and uniformity analysis. Clustering analysis showed that 43 varieties (lines) were completely differed from each other with 30 markers. Varieties (lines) from the same breeding institute tended to share a higher genetic similarity, while varieties (lines) with different origins seemed to be more distinct. With a genetic similarity coefficient threshold of 0.90, 90% of varieties (lines) were distinctive, and 80% of tested varieties (lines) were classified into high uniformity group in consistence with marker loci uniformity, Furthermore, in assignment test, 86% of derivative individuals were correctly corresponding to their original varieties (lines), it indicated that most varieties (lines) have a good uniformity. Overall, the results showed that most of the tested sesame varieties (lines) have good distinction and uniformity, and SSR marker is suitable for variety identification and protection.

Key words: Sesame variety, SSR, Distinctness, Uniformity

[1]Ashri A. Sesame breeding. Plant Breed Rev, 1998, 16: 179–228

[2]Sun J(孙建), Zhang X-R(张秀荣), Zhang Y-X(张艳欣), Che Z(车卓), Huang B(黄波). Analysis on genetic diversity and genetic basis of main sesame cultivars released in China. Sci Agric Sin (中国农业科学), 2009, 42(10): 3421–3431 (in Chinese with English abstract)

[3]Ma S-W(马双武). Research and application progress of seedling marker character in crop breeding and seed purity identification in China. J Plant Genet Resour (植物遗传资源学报), 2011, 12( 2): 297–300 (in Chinese with English abstract)

[4]Mei D-S(梅德圣), Li Y-C(李云昌), Chen Y-F(陈玉峰), Li Y-D(李英德), Xu Y-S(徐育松), Hu Q(胡琼). Seed purity identification of Zhongyouza 12 by peroxidase isozyme and SSR markers in Brassica napus. Chin J Agric Biotechnol (农业生物技术学报), 2010, 18(4): 815–821 (in Chinese with English abstract)

[5]Fang Y-C(方玉春), Zhang X-H(张兴和), Feng D-J(冯德举), Liu J-P(刘俊平), Zhuang B-C(庄炳昌), Liang X-K(梁晓葵). The identification of corn seed purity by using PAGE patterns of salt soluble protein. J Jilin Agric Univ (吉林农业大学学报), 2001, 23(4): 6–10 (in Chinese with English abstract)

[6]Smith J S C, Chin E C L, Shu H, Smith O S. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): Comparison with data from RFLPs and pedigree. Theor App1 Genet, 1997, 95: 163–173

[7]Wang F-G(王风格), Zhao J-R(赵久然), Guo J-L(郭景伦), She H-D(佘花娣), Chen G(陈刚). Comparison of three DNA fingerprint analyzing methods for maize cultivars’ identification. Mol Plant Breed (分子植物育种), 2003, 1(5): 655–661 (in Chinese with English abstract)

[8]Li L(李莉), Yang J-B(杨剑波), Machill D J, Colowit P M. Comparison of different detection methods for rice SSR analysis. Chin J Rice Sci (中国水稻科学), 2000, l4(3): 185–l88 (in Chinese with English abstract)

[9]Li W(李伟), Zheng Y-L(郑有良), Wei Y-M(魏育明), Yan Z-H(颜泽洪), Lan X-J(兰秀锦). Molecular identification of new wheat cultivar Chuannong 16. J Triticeae Crops (麦类作物学报), 2004, 24(1): 6–l0 (in Chinese with English abstract)

[10]Lu G-Y(陆光远), Wu X-M(伍晓明), Zhang D-X(张冬晓), Liu F-L(刘凤兰), Chen B-Y(陈碧云), Gao G-Z(高桂珍), Xu K(许鲲). SSR-based evaluation of distinctness and uniformity of rapeseed (Brassica napus L.) varieties under Chinese National Official Field Tests. Sci Agric Sin (中国农业科学), 2008, 41(1): 32–42 (in Chinese with English abstract)

[11]Luan Y-S(栾雨时), An L-J(安利佳), Wang B-Q(黄百渠), Zhang W-J(张文俊), He M-Y(何孟元). Using simple sequence repeat probe for DNA fingerprints in tomato cultivars. Acta Hort Sin (园艺学报), 1999, 26(1): 51–53 (in Chinese with English abstract)

[12]Cui Y-H(崔艳华), Qiu L-J(邱丽娟), Chang R-Z(常汝镇), Lü W-H(吕文河). Examination of representiveness of the primary core collection in Huanghuai summer sowing soybean (Glycine max) using SSR. J Plant Genet Resour (植物遗传资源学报), 2003, 4(1): 9–l5 (in Chinese with English abstract)

[13]Dixit A, Jin M H, Chung J W, Yu J W, Chung H K, Ma K H, Park Y J, Cho E G. Development of polymorphic microsatellite markers in sesame (Sesamum indicum L.). Mol Ecol Notes, 2005, 5: 736–738

[14]Wei L-B(魏利斌), Zhang H-Y(张海洋), Zheng Y-Z(郑永战), Guo W-Z(郭旺珍), Zhang T-Z(张天真). Development and utilization of EST-derived microsatellites in sesame (Sesamum indicum L.). Acta Agron Sin (作物学报), 2008, 34(12): 2077–2084 (in Chinese with English abstract)

[15]Lu G-Y(陆光远), Yang G-S(杨光圣), Fu T- D(傅廷栋). An effective assay of detecting SSR marker in rapeseed. Chin J Oil Crops Sci (中国油料作物学报), 2003, 25: 79–81 (in Chinese with English abstract)

[16]Zhang B(张鹏), Zhang H-Y(张海洋), Guo W-Z(郭旺珍), Zheng Y-Z(郑永战), Wei L-B(魏利斌), Zhang T-Z(张天真). Genetic diversity analysis of Sesamum indicum L. germplasms using SRAP and EST-SSR markers. Acta Agron Sin (作物学报), 2007, 33 (10): 1696–1702 (in Chinese with English abstract)

[17]Lombard V, Baril C P, Dubreuil P, Blouet F, Zhang D. Genetic relationships and fingerprinting of rapeseed cultivars by AFLP: consequences for varietal registration. Crop sci, 2000, 40: 1417–1425

[18]Piry S, Alapetite A, Cornuet J M, Paetkau D, Baudouin L, Estoup A. GeneClass2: a software for genetic assignment and first-generation migrant detection. J Heredity, 2004, 95: 536–539

[19]Troyer A F, Rocheford T R. Germplasm ownership: related corn inbreds. Crop Sci, 2002, 42: 3–10

[20]LI J-F(李俊芳), Zhang X-Y(张雪原), Sun S-X(孙世贤), Wang S-C(王守才). Analysis of maize variety in national maize main production area using SSR technique: Ⅰ. Evaluation of distinctness and uniformity of maize variety. J Maize Sci (玉米科学), 2006, 14(6): 38–42 (in Chinese with English abstract)

[21]Heckenberger M, van der Voort J R, Melchinger A E, Peleman J, Bohn M. Variation of DNA fingerprints among accessions within maize inbred lines and implications for identification of essentially derived varieties: II. Genetic and technical sources of variation in AFLP data and comparison with SSR data. Mol Breed, 2003, 12: 97–106.

[22]Smith J S C, Smith O S. The description and assessment of distances between inbred lines of maize: II. The utility of morphological, biochemical, and genetic descriptors and a scheme for testing of distinctiveness between inbred lines. Maydica, 1989, 34: 151–161

[23]ASSINSEL. 2000. DUS testing: Phenotype vs. genotype [Online]. ASSINSEL, Position Paper adopted at the Rome Congress in May 2000. http://www.worldseed.org/Position_papers/PhenotypeGenotype.htm [verified 30 Nov. 2004]. International Association of Plant Breeders for the Protection of Plant Varieties (ASSINSEL), Nyon, Switzerland
[1] CHEN Xiao-Hong, LIN Yuan-Xiang, WANG Qian, DING Min, WANG Hai-Gang, CHEN Ling, GAO Zhi-Jun, WANG Rui-Yun, QIAO Zhi-Jun. Development of DNA molecular ID card in hog millet germplasm based on high motif SSR [J]. Acta Agronomica Sinica, 2022, 48(4): 908-919.
[2] ZHANG Xia, YU Zhuo, JIN Xing-Hong, YU Xiao-Xia, LI Jing-Wei, LI Jia-Qi. Development and characterization analysis of potato SSR primers and the amplification research in colored potato materials [J]. Acta Agronomica Sinica, 2022, 48(4): 920-929.
[3] WANG Yan-Yan, WANG Jun, LIU Guo-Xiang, ZHONG Qiu, ZHANG Hua-Shu, LUO Zheng-Zhen, CHEN Zhi-Hua, DAI Pei-Gang, TONG Ying, LI Yuan, JIANG Xun, ZHANG Xing-Wei, YANG Ai-Guo. Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources [J]. Acta Agronomica Sinica, 2021, 47(7): 1259-1274.
[4] HAN Bei, WANG Xu-Wen, LI Bao-Qi, YU Yu, TIAN Qin, YANG Xi-Yan. Association analysis of drought tolerance traits of upland cotton accessions (Gossypium hirsutum L.) [J]. Acta Agronomica Sinica, 2021, 47(3): 438-450.
[5] LIU Shao-Rong, YANG Yang, TIAN Hong-Li, YI Hong-Mei, WANG Lu, KANG Ding-Ming, FANG Ya-Ming, REN Jie, JIANG Bin, GE Jian-Rong, CHENG Guang-Lei, WANG Feng-Ge. Genetic diversity analysis of silage corn varieties based on agronomic and quality traits and SSR markers [J]. Acta Agronomica Sinica, 2021, 47(12): 2362-2370.
[6] GUO Yan-Chun, ZHANG Li-Lan, CHEN Si-Yuan, QI Jian-Min, FANG Ping-Ping, TAO Ai-Fen, ZHANG Lie-Mei, ZHANG Li-Wu. Establishment of DNA molecular fingerprint of applied core germplasm in jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2021, 47(1): 80-93.
[7] WANG Heng-Bo,QI Shu-Ting,CHEN Shu-Qi,GUO Jin-Long,QUE You-Xiong. Development and application of SSR loci in monoploid reference genome of sugarcane cultivar [J]. Acta Agronomica Sinica, 2020, 46(4): 631-642.
[8] Hong-Yan ZHANG,Tao YANG,Rong LIU,Fang JIN,Li-Ke ZHANG,Hai-Tian YU,Jin-Guo HU,Feng YANG,Dong WANG,Yu-Hua HE,Xu-Xiao ZONG. Assessment of genetic diversity by using EST-SSR markers in Lupinus [J]. Acta Agronomica Sinica, 2020, 46(3): 330-340.
[9] Li-Lan ZHANG, Lie-Mei ZHANG, Huan-Ying NIU, Yi XU, Yu LI, Jian-Min QI, Ai-Fen TAO, Ping-Ping FANG, Li-Wu ZHANG. Correlation between SSR markers and fiber yield related traits in jute (Corchorus spp.) [J]. Acta Agronomica Sinica, 2020, 46(12): 1905-1913.
[10] LIU Rong, WANG Fang, FANG Li, YANG Tao, ZHANG Hong-Yan, HUANG Yu-Ning, WANG Dong, JI Yi-Shan, XU Dong-Xu, LI Guan, GUO Rui-Jun, ZONG Xu-Xiao. An integrated high-density SSR genetic linkage map from two F2 population in Chinese pea [J]. Acta Agronomica Sinica, 2020, 46(10): 1496-1506.
[11] YE Wei-Jun,CHEN Sheng-Nan,YANG Yong,ZHANG Li-Ya,TIAN Dong-Feng,ZHANG Lei,ZHOU Bin. Development of SSR markers and genetic diversity analysis in mung bean [J]. Acta Agronomica Sinica, 2019, 45(8): 1176-1188.
[12] Zhi-Jun TONG,Yi-Han ZHANG,Xue-Jun CHEN,Jian-Min ZENG,Dun-Huang FANG,Bing-Guang XIAO. Mapping of quantitative trait loci conferring resistance to brown spot in cigar tobacco cultivar Beinhart1000-1 [J]. Acta Agronomica Sinica, 2019, 45(3): 477-482.
[13] CHEN Fang,QIAO Lin-Yi,LI Rui,LIU Cheng,LI Xin,GUO Hui-Juan,ZHANG Shu-Wei,CHANG Li-Fang,LI Dong-Fang,YAN Xiao-Tao,REN Yong-Kang,ZHANG Xiao-Jun,CHANG Zhi-Jian. Genetic analysis and chromosomal localization of powdery mildew resistance gene in wheat germplasm CH1357 [J]. Acta Agronomica Sinica, 2019, 45(10): 1503-1510.
[14] XUE Yan-Tao,LU Ping,SHI Meng-Sha,SUN Hao-Yue,LIU Min-Xuan,WANG Rui-Yun. Genetic diversity and population genetic structure of broomcorn millet accessions in Xinjiang and Gansu [J]. Acta Agronomica Sinica, 2019, 45(10): 1511-1521.
[15] Jia-Yu YAO,Li-Wu ZHANG,Jie ZHAO,Yi XU,Jian-Min QI,Lie-Mei ZHANG. Evaluation and characteristic analysis of SSRs from the whole genome of jute (Corchorus capsularis) [J]. Acta Agronomica Sinica, 2019, 45(1): 10-17.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd