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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (04): 589-595.doi: 10.3724/SP.J.1006.2012.00589

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Chromosome Location and Molecular Mapping of Powdery Mildew Resistance Gene PmAS846 Originated from Wild Emmer (Triticum turgidum var. dicoccoides)

XUE Fei1,WANG Chang-You1,ZHANG Li-Hua2,ZHANG Hong1,LI Hao1,WANG Ya-Juan1,LIU Xin-Lun1,JI Wan-Quan1,*   

  1. 1 College of Agronomy, Northwest A&F University / State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China; 2 Seed Technology Extension Station of Neixiang Country in Henan Province, Neixiang 474350, China
  • Received:2011-08-08 Revised:2011-12-19 Online:2012-04-12 Published:2012-02-14
  • Contact: 王长有, E-mail: chywang2004@126.com; 吉万全, E-mail: jiwanquan2003@126.com

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Abstract: Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most important diseases of wheat (Triticum aestivum L.) worldwide. Wheat relativesare important donors of resistance genes against this disease in wheat breeding program. The common wheat line N9738 is highly resistant to Bgt isolate E09 and Shaanxi prevailing races at both seedling and adult plant stages. The PmAS846 gene in line N9738 was derived from wild emmer (Triticum turgidum var. dicoccoides) accession As846. Genetic analysis of an F2 population and their F3 families, developed from the cross between N9738 and a susceptible common wheat cultivar Huixianhong, indicated that N9738 carries one dominant resistance gene. A set of common wheat nullisomic (monosomic) lines were used to analyze the chromosomal location of PmAS846. The results revealed that PmAS846 was located on wheat chromosome 5B. Microsatellite markers on wheat chromosome 5B were used to map the gene using bulked segregant analysis. Eleven microsatellite markers were used to construct a linkage map for the gene, and two markers, Xgpw3191 and Xfcp1, ?anking PmAS846 location at 7.3 and 1.8 cM, respectively. Amplification of 5B chromosome deletion lines of Chinese Spring with the flanking markers mapped PmAS846 on chromosome 5BL bin 0.75–0.76. Based on expressed sequence tags (ESTs) information mapped on chromosome 5BL bin 0.75–0.76, we identified EST-derived sequence tagged site (STS) markers BF202652 and BF482522 to be closely linked to PmAS846 with genetic distance of 5.1 cM both. These markers can be used in fine mapping of PmAS846 and marker-assisted selection.

Key words: Powdery mildew, Wild emmer, Resistance gene, Chromosome location, Molecular markers

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