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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (04): 596-605.doi: 10.3724/SP.J.1006.2012.00596

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

DNA Fingerprinting of Sesame (Sesamum indicum L.) Varieties (Lines) from Recent National Regional Trials in China

LIU Hong-Yan,WU Kun,YANG Min-Min,ZUO Yang,ZHAO Ying-Zhong*   

  1. Key Laboratory of Oil Crops Biology and genetic breeding of the Ministry of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Science, Wuhan 430062, China
  • Received:2011-08-17 Revised:2011-12-19 Online:2012-04-12 Published:2012-02-13
  • Contact: 赵应忠, E-mail: yingzhzh@public.wh.hb.cn

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Abstract: DNA fingerprinting is an important technique for plant variety identification and protection. In this study, 43 sesame (Sesamum indicum L.) varieties (lines) from recent National Regional Trail were amplificated with 12 pairs of SSR primers, and a total of 30 polymorphic bands were obtained. These molecular markers were further used for variety (lines) distinction and uniformity analysis. Clustering analysis showed that 43 varieties (lines) were completely differed from each other with 30 markers. Varieties (lines) from the same breeding institute tended to share a higher genetic similarity, while varieties (lines) with different origins seemed to be more distinct. With a genetic similarity coefficient threshold of 0.90, 90% of varieties (lines) were distinctive, and 80% of tested varieties (lines) were classified into high uniformity group in consistence with marker loci uniformity, Furthermore, in assignment test, 86% of derivative individuals were correctly corresponding to their original varieties (lines), it indicated that most varieties (lines) have a good uniformity. Overall, the results showed that most of the tested sesame varieties (lines) have good distinction and uniformity, and SSR marker is suitable for variety identification and protection.

Key words: Sesame variety, SSR, Distinctness, Uniformity

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