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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (06): 996-1002.doi: 10.3724/SP.J.1006.2012.00996

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genomic Variation in F1 Hybrid and Amphidiploid between Aegilops tauschii and Secale cereale

LI Suo-Ping1,2,ZHANG Da-Le2,WANG Xiu-E1,QI Zeng-Jun1,LIU Da-Jun1,*   

  1. 1 National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2 State Key Laboratory of Cotton Biology, Henan University, Kaifeng 475001, China
  • Received:2011-09-08 Revised:2012-02-22 Online:2012-06-12 Published:2012-03-29
  • Contact: 刘大钧, E-mail: djliu@njau.edu.cn

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Abstract: Synthetic amphidiploids play an important role in genetic study and plant breeding. To understand the molecular mechanism of fertility improvement and cytological stability in amphidiploid, we analyzed the fertility, genomic variation, and cytology of the F1 hybrid and S1 to S4 amphidiploid progenies from the cross between Aegilops tauschii and Secale cereale by means of microscopic observation and molecular markers (ALFP and MASP). The percentage of plants with chromosome number of 2n = 28 increased from 57.1% to 92.5% in the amphidiploid progenies. The bivalent in pollen mother cells (PMCs) at meiosis I stage increased from 11.70 to 12.25 averagely, and the mean rate of seed setting was enhanced from 24.5% to 51.3% in the amphidiploid progenies (2n=28). Genomic variation in the F1 hybrid and the amphidiploids was detected using AFLP markers digested by EcoRI/Mse I (E-M) and Pst I/Mse I (P-M), which could primarily amplify repetitive and low-copy sequences, respectively. The results showed that the genomic variation occurred mainly in F1 hybrid with dominant form of sequence elimination. The loss percentages in F1 plants were 70.00% at E-M locus and 52.95% at P-M locus for the Ae. tauschii banding pattern and 96.88% at E-M locus and 81.64% at P-M locus for the S. cereale banding pattern. The result of MSAP analysis showed that the cytosine methylation alterations, mainly methylated, only occurred in the F1 hybrids and the S1 amphidiploids. In S2 to S4 amphidiploids, no methylation was observed.

Key words: Amphidiploid, Genomic sequence variation, Aegilops tauschii, Secale cereale, AFLP, MSAP

[1]Masterson J. Stomatal size in fossil plants: evidence for polyploidy in the majority of angiosperms. Science, 1994, 264: 421–424

[2]Wendel J F. Genome evolution in polyploidy. Plant Mol Biol, 2000, 42: 225–249

[3]Guyot R, Keller B. Ancestral genome duplication in rice. Genome, 2004, 47: 610–614

[4]Gaut B S, Doebley J F. DNA sequence evidence for the segmental allotetraploid origin of maize. Proc Natl Acad Sci USA, 1997, 94: 6809–6814

[5]Shoemaker R C, Polzin K, Labate J, Specht J, Brummer E C, Olson T, Young N, Concibido V, Wilcox J, Tamulonis J P, Kochert G, Boerma H R. Genome duplication in soybean (Glycine subgenus soja). Genetics, 1996, 144: 329–338

[6]Vision T J, Brown D G, Tanksley S D. The origins of genomic duplications in Arabidopsis. Science, 2000, 290: 2114–2117

[7]Arabidopsis genome initiative: analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature, 2000, 408: 796–815

[8]Song K, Lu P, Tang K, Osborn T C. Rapid genome change in synthetic polyploids of Brassica and its implications for polyploidy evolution. Proc Natl Acad Sci USA, 1995, 92: 7719–7723

[9]Ozkan H, Levy A A, Feldman M. Allopolyploidy-induced rapid genome evolution in the wheat (Aegilops-Triticum) group. Plant Cell, 2001, 13: 1735–1747

[10]Comai L, Yyagi A P, Winter K. Phenotypical instability and rapid gene silencing in newly formed Arabidopsis allotetraploids. Plant Cell, 2000, 12: 1551–1567

[11]Madlung A, Masuelli R W, Watson B, Reynolds S H, Davison J, Comai L. Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids. Plant Physiol, 2002, 129: 733–746

[12]Liu B, Brubaker C L, Mergeai G, Cronn R C, Wendel J F. Polyploid formation in cotton is not accompanied by rapid genome changes. Genome, 2001, 44: 321–330

[13]Baumel A, Ainouche M L, Kalendar J E, Schulman A H. Retrotransposons and genomic stability in populations of the young allopolyploid species Spartina anglica C. E. Hubbard (Poaceae). Mol Biol Evol, 2002, 19: 1218–1227

[14]Axelsson T, Bowman C M, Sharpe A G, Lydiate D J, Lagercrantz U. Amphidiploid Brassica juncea contains conserved progenitor genomes, Genome, 2000, 43: 679–688

[15]Wilson A S. Wheat and rye hybrids. Edinburgh Bat Sac Trans, 1876, 12: 286–288

[16]Ma X F, Fang P, Gustafson J P. Polyploidization-induced genome variation in triticale. Genome, 2004, 47: 839–848

[17]Ma X F, Gustafson J P. Timing and rate of genome variation in Triticale following allpolyploidization. Genome, 2006, 49: 950–985

[18]Doyle J J, Doyle J L. A rapid DNA isolation procedure from small quantities of fresh leaf tissue. Phytochem Bull, 1987, 19: 11–15

[19]Feldman M, Liu B, Segal G, Abbo S, Levy A A, Vega J M. Rapid elimination of low-copy DNA sequences in polyploid wheat: a possible mechanism for differentiation of homoeologous chromosomes. Genetics, 1997, 147: 1381–1387

[20]Liu B, Vega J M, Feldman M. Rapid genomic changes in newly synthesized amphiploids of Triticum and Aegilops: II. Changes in low-copy coding DNA sequences. Genome, 1998, 41: 535–542

[21]Shaked H, Kashkush K, Ozkan H, Feldman M, Levy A A. Sequence elimination and cytosine methylation are rapid and reproducible responses of the genome to wide hybridization and allopolyploidy in wheat. Plant Cell, 2001, 13: 1749–1759

[22]Liu B, Vega J M, Segal G, Abbo S, Rodova M, Feldman M. Rapid genomic changes in newly synthesized amphiploids of Triticum and Aegilops: I. Changes in low-copy noncoding DNA sequences. Genome, 1998, 41: 271–277

[23]Liu B, Hu B, Dong Y Z, Liu Z L, He M Y. Speciation-induced heritable cytosine methylation changes in ployploid wheat. Prog Nat Sci, 2000, 10: 601–606

[24]Bao W-K(鲍文奎). Challenge and risk: thoughts on 40 years’ research of crop breeding. Plants (植物杂志), 1990, 17(4): 4–5 (in Chinese)

[25]Zhang X-Y(张学勇), Li D-Y(李大勇). Repetitive DNA sequences in wheat and its relatives. Sci Agric Sin (中国农业科学), 2000, 33(5): 1–7 (in Chinese with English abstract)
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