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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (06): 996-1002.doi: 10.3724/SP.J.1006.2012.00996

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genomic Variation in F1 Hybrid and Amphidiploid between Aegilops tauschii and Secale cereale

LI Suo-Ping1,2,ZHANG Da-Le2,WANG Xiu-E1,QI Zeng-Jun1,LIU Da-Jun1,*   

  1. 1 National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2 State Key Laboratory of Cotton Biology, Henan University, Kaifeng 475001, China
  • Received:2011-09-08 Revised:2012-02-22 Online:2012-06-12 Published:2012-03-29
  • Contact: 刘大钧, E-mail: djliu@njau.edu.cn

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Abstract: Synthetic amphidiploids play an important role in genetic study and plant breeding. To understand the molecular mechanism of fertility improvement and cytological stability in amphidiploid, we analyzed the fertility, genomic variation, and cytology of the F1 hybrid and S1 to S4 amphidiploid progenies from the cross between Aegilops tauschii and Secale cereale by means of microscopic observation and molecular markers (ALFP and MASP). The percentage of plants with chromosome number of 2n = 28 increased from 57.1% to 92.5% in the amphidiploid progenies. The bivalent in pollen mother cells (PMCs) at meiosis I stage increased from 11.70 to 12.25 averagely, and the mean rate of seed setting was enhanced from 24.5% to 51.3% in the amphidiploid progenies (2n=28). Genomic variation in the F1 hybrid and the amphidiploids was detected using AFLP markers digested by EcoRI/Mse I (E-M) and Pst I/Mse I (P-M), which could primarily amplify repetitive and low-copy sequences, respectively. The results showed that the genomic variation occurred mainly in F1 hybrid with dominant form of sequence elimination. The loss percentages in F1 plants were 70.00% at E-M locus and 52.95% at P-M locus for the Ae. tauschii banding pattern and 96.88% at E-M locus and 81.64% at P-M locus for the S. cereale banding pattern. The result of MSAP analysis showed that the cytosine methylation alterations, mainly methylated, only occurred in the F1 hybrids and the S1 amphidiploids. In S2 to S4 amphidiploids, no methylation was observed.

Key words: Amphidiploid, Genomic sequence variation, Aegilops tauschii, Secale cereale, AFLP, MSAP

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