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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (10): 1877-1883.doi: 10.3724/SP.J.1006.2014.01877

• RESEARCH NOTES • Previous Articles    

Genetic Diversity Analysis of 118 Sugarcane Germplasm with AFLP Markers

ZAN Feng-Gang,WU Cai-Wen,CHEN Xue-Kuan,ZHAO Pei-Fan,ZHAO Jun,LIU Jia-Yong*   

  1. Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences / Yunnan Province Key Laboratory of Sugarcane Genetic Improvement, Kuaiyuan 616699, China
  • Received:2014-04-04 Revised:2014-07-06 Online:2014-10-12 Published:2014-07-25

Abstract:

AFLP markers were used to assess the genetic diversity of 118 sugarcane germplasm from China, Australia, USA, Philippines, Brazil, and France. A total of 1310 bands were amplified with ten pairs of primers, 1195 of which were polymorphic bands, the percentage of polymorphic loci was 91.22%. The genetic similarity coefficient among 118 sugarcane germplasm ranged from 0.583 to 0.929 with an average of 0.750, the polymorphism information content was 0.2332, and the effective number of alleles per loci was 1.3789. The results showed 118 germplasm were divided into two groups at 0.69 of the similarity coefficient. The group I consisted of YT00-236, YZ04-622 and SP80-1816, which shared lower genetic similarity with the rest of 115 germplasm in another group. The 115 germplasm were divided into five sub-groups at 0.74 of the similarity coefficient. The sugarcane germplasm shared high genetic similarity among countries, and there existed much higher genetic diversity within germplasm introduced from USA.

Key words: Sugarcane, Germplasm, AFLP, Genetic diversity

[1] 李奇伟, 陈子云, 梁洪.现代甘蔗改良技术. 广州: 华南理工大学出版社, 2000. pp 11–18

Li Q W, Chen Z Y, Liang H. Modern Technology for Sugarcane Improvement. Guangzhou: South China University of Technology Press, 2000. pp 11–18 (in Chinese)

[2] 邓海华, 李奇伟. CP72-1210在我国甘蔗育种中的利用. 广东农业科学, 2007, 11: 18–21

Deng H H, Li Q W. Utilization of CP72-1210 in sugarcane breeding program in mainland China. Guangdong J Agric Sci, 2007, 11: 18–21 (in Chinese with English abstract)

[3] AitkenK S, Li J C, Jackson P, Piperidis G, McIntyre C L. AFLP analysis of genetic diversity within Saccharum officinarum and comparison with sugarcane cultivars. Austr J Agric Res, 2006, 57: 1167–1184

[4] Selvi A, Nair N V, Noyer J L, Singh N K, Balasundaram N, Bansal K C, Koundal K R, Mohapatra T. AFLP analysis of the phenetic organization and genetic diversity in the sugarcane complex, Saccharum and Erianthus. Genet Resour Crop Evol, 2006, 53: 831–842

[5] Harvey M, Huckett B I, Botha F C. Use of Polymerase Chain Reaction (PCR), and Random Amplification of Polymorphic DNAs, RAPDs, for the Determination of Genetic Distances between 21 Sugarcane Varieties. In: Proceeding of South African Sugar Technologists Association, 1994, 68: 36–40

[6] Nair N V, Selvi A, Sreenivasan T V, Pushpalatha K N. Molecular diversity in Indian sugarcane cultivars as revealed by randomly amplified DNA polymorphisms. Euphytica, 2002, 127: 219–225

[7] Lima M L A, Garcia A A F, Oliveira K M, Matsuoka S, Arizono H, Souza C L J, Souza A P. Analysis of genetic similarity detected by AFLP and coefficient of parentage among genotypes of sugar cane (Saccharum spp.). Theor Appl Genet, 2002, 104: 30–38

[8] 庄南生, 郑成木, 黄东益, 唐燕琼, 高和琼. 甘蔗种质遗传基础的AFLP分析. 作物学报, 2005, 31: 444–450

Zhuang N S, Zheng C M, Huang D Y, Tang Y Q, Gao H Q. AFLP analysis for sugarcane germplasms. Acta Agron Sin, 2005, 31: 444–450 (in Chinese with English abstract)

[9] 刘家勇, 赵培方, 刘新龙, 赵俊, 杨昆, 吴才文, 应雄美, 昝逢刚, 陈学宽. 68份国外甘蔗种质资源遗传多样性的AFLP分析. 湖南农业大学学报, 2013, 39: 466–470

Liu J Y, Zhao P F, Liu X L, Zhao J, Yang K, Wu C W, Ying X M, Zan F G, Chen X K. Genetic diversity analysis on 68 foreign sugarcane germplasms (Saccharum spp.) with AFLP technique. J Hunan Agric Univ, 2013, 39: 466–470 (in Chinese with English abstract)

[10] Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M. AFLP: a new technique for DNA fingerprinting. Nucl Acids Res, 1995, 23: 4407–4414

[11] 劳方业, 刘睿, 何慧怡, 邓海华, 陈仲华, 陈健文, 符成, 张垂明, 杨业后. 崖城系列甘蔗亲本遗传多样性的AFLP标记分析. 分子植物育种, 2008, 6: 517–522

Lao F Y, Liu R, He H Y, Deng H H, Chen Z H, Chen J W, Fu C, Zhang C M, Yang Y H. AFLP analysis of genetic diversity in series sugarcane parents developed at HSBS. Mol Plant Breed, 2008, 6: 517–522 (in Chinese with English abstract)

[12] Schenck S, Crepeau M W, Wu K K, Moore P H, Yu Q, Ming R. Genetic diversity and relationships in native Hawaiian Saccharum officinarum sugarcane. J Hered, 2004, 95: 327–331

[13] 李鸣, 谭裕模, 李杨瑞, 李容柏, 高国庆. 甘蔗(Saccharum officinarum L.)品种遗传差异的AFLP分子标记分析. 作物学报, 2004, 30: 1008–1013

Li M, Tan Y M, Li Y R, Li R B, Gao G Q. AFLP molecular analysis of genetic difference between cultivars in sugarcane (Saccharum officinarum L). Acta Agron Sin, 2004, 30: 1008–1013 (in Chinese with English abstract)

[14] 刘新龙, 毛钧, 陆鑫, 马丽, Karen S A, Phillip A J, 蔡青, 范源洪. 甘蔗SSR和AFLP分子遗传连锁图谱构建. 作物学报, 2010, 36: 177–183

Liu X L, Mao J, Lu X, Ma L, Karen S A, Phillip A J, Cai Q, Fan Y H. Construction of molecular genetic linkage map of sugarcane based on SSR and AFLP markers. Acta Agron Sin, 2010, 36: 177–183 (in Chinese with English abstract)

[15] 吴转娣, 昝逢刚, 赵丽宏, 罗遵喜, 张树珍. 甘蔗基因组DNA小量提取与大量提取方法研究. 生物技术通报, 2009, (增刊-1): 172–175

Wu Z Z, Zan F G, Zhao L H, Luo Z X, Zhang S Z. Study on mini and mass-extraction methods of sugarcane genomic DNA. Biotechnol Bull, 2009, (suppl-1): 172–175 (in Chinese with English abstract)

[16] 刘新龙, 蔡青, 毕燕, 陆鑫, 马丽, 应雄美, 毛钧. 甘蔗AFLP标记和SSR标记的PAGE胶快速银染检测方法. 江苏农业学报, 2009, 25: 433–435

Liu X L, Cai Q, Bi Y, Lu X, Ma L, Ying X M, Mao J. A rapid silver staining method for PAGE used in sugarcane AFLP and SSR molecular markers. Jiangsu J Agric Sci, 2009, 25: 433–435 (in Chinese with English abstract)

[17] 劳方业, 刘睿, 何慧怡, 邓海华, 李奇伟, 陈仲华, 陈健文, 符成, 齐永文, 张垂明. 我国甘蔗亲本遗传多样性的AFLP标记分析. 基因组学与应用生物学, 2009, 28: 503–508

Lao F Y, Liu R, He H Y, Deng H H, Li Q W, Chen Z H, Chen J W, Fu C, Qi Y W, Zhang C M. Genetic diversity analysis of sugarcane parents with AFLP in China. Genom Appl Biol, 2009, 28: 503–508 (in Chinese with English abstract)

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