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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (10): 1877-1883.doi: 10.3724/SP.J.1006.2014.01877

• RESEARCH NOTES • Previous Articles    

Genetic Diversity Analysis of 118 Sugarcane Germplasm with AFLP Markers

ZAN Feng-Gang,WU Cai-Wen,CHEN Xue-Kuan,ZHAO Pei-Fan,ZHAO Jun,LIU Jia-Yong*   

  1. Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences / Yunnan Province Key Laboratory of Sugarcane Genetic Improvement, Kuaiyuan 616699, China
  • Received:2014-04-04 Revised:2014-07-06 Online:2014-10-12 Published:2014-07-25

Abstract:

AFLP markers were used to assess the genetic diversity of 118 sugarcane germplasm from China, Australia, USA, Philippines, Brazil, and France. A total of 1310 bands were amplified with ten pairs of primers, 1195 of which were polymorphic bands, the percentage of polymorphic loci was 91.22%. The genetic similarity coefficient among 118 sugarcane germplasm ranged from 0.583 to 0.929 with an average of 0.750, the polymorphism information content was 0.2332, and the effective number of alleles per loci was 1.3789. The results showed 118 germplasm were divided into two groups at 0.69 of the similarity coefficient. The group I consisted of YT00-236, YZ04-622 and SP80-1816, which shared lower genetic similarity with the rest of 115 germplasm in another group. The 115 germplasm were divided into five sub-groups at 0.74 of the similarity coefficient. The sugarcane germplasm shared high genetic similarity among countries, and there existed much higher genetic diversity within germplasm introduced from USA.

Key words: Sugarcane, Germplasm, AFLP, Genetic diversity

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