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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (06): 1018-1028.doi: 10.3724/SP.J.1006.2012.01018

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Diversity of SSR Marker in 175 Wheat Varieties from Huang-Huai Winter Wheat Region and Its Association with Plant Height and Yield Related Traits

WU Yu-Guo,WU Cheng-Lai,QIN Bao-Ping,WANG Zhen-Lin,HUANG Wei,YANG Min,YIN Yan-Ping*   

  1. State Key Laboratory of Crop Biology / Shandong Provincial Key Laboratory of Crop Biology / Agronomy College of Shandong Agricultural University, Tai’an 271018, China
  • Received:2011-11-28 Revised:2012-01-19 Online:2012-06-12 Published:2012-03-05
  • Contact: 尹燕枰, E-mail: ypyinsdau@sina.com, Tel: 0538-8242458

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Abstract: To make advances in wheat breeding, it is important to study germplasm diversity of Huang-huai winter wheat, and identify SSR markers associated with important agronomic traits, including plant heightand yield related traits. In this study, we selected 175 historical winter wheat varieties planted in Huang-Huai winter wheatarea and 108 pairs of SSR markers evenly distributed on the chromosomes of wheat to reveal the genetic diversity. A total of 448 alleles of loci were detected in the experimental varieties. The average alleles per locus were 4.15, which varied from 2 to 14. Thepolymorphism information content (PIC) value ranged from 0.075 to 0.869, with an average of 0.560. Cluster analysis showed that most of the varieties with the common parents, bred by the same breeders and planted in the same area were clustered together. A total of 23 were markers significantly (P<0.01) associated with plant height and yield related traits, among which threewere very significantly(P<0.001) associated with the traits.The markerswmc128 (1B) and wmc236 (3B) could explain 10.5% and 8.0% of variation in the number of spikelets(P<0.001), respectively.And, Xgwm129 (2B) was significantly (P<0.001) associated with 1000- kernel weight, whose phenotypic variation explaining rate was as high as 19.3%.

Key words: Wheat, Genetic diversity, SSR, Allele, Association analysis

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