桑树花青素合酶基因的克隆与信息学分析

doi:10.3724/SP.J.1006.2012.01253

Abstract

Abstract: Anthocyanidin synthase (ANS, leucoanthocyanidin oxygenase) is one of the critical enzymes in the biosynthesis of the anthocyanin. Anthocyanidin synthase gene fragment (designated as MaANS) was isolated from mulberry fruit (Moru alba) by RT-PCR based on homology cloning and genome walking technology. MaANS with the 5′ and 3′ was cloned by genome-walking. The full-length genomic sequence of MaANS is 1 535 bp, which consists of two exons and one intron. The coding region length was
1 077 bp, and their deduced protein consisted of 358 amino acid residues. Multiple alignments revealed that the nucleic acid of MaANS shared above 80% identity with that of Fragaria × ananassa, Ipomoea batatas, Malus domestica, and Pyrus pyrifolia. Structural analysis showed that the MaANS protein might belong to the 2 OG and Fe(II)-dependent oxygenase family. Phylogenetic tree analysis revealed that MaANS was the most close with Fragaria × ananassa, then Malus domestica and Pyrus pyrifolia. Reverse transcription-PCR (RT-PCR) analyses of MaANS transcripts showed that it was abundantly expressed in the young leaves and ripened fruit. All research made an essential foundation for pathway and regulation of gene expression in anthocyanin biosynthesis in mulberry fruit.

Key words: Mulberry, Anthocyanin synthase, Cloning, Information analysis, Tissues expression

ZHANG Qiong-Yu, LI Jun, ZHAO Ai-Chun, WANG Xi-Ling, JIN Xiao-Yun, Li Zhen-Gang,YU Mao-De. Molecular Cloning and Information Analysis of ANS Genes Encoding Anthocyanin Synthases from Mulberry (Morus alba)[J].Acta Agron Sin, 2012, 38(07): 1253-1263.

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Molecular Cloning and Information Analysis of ANS Genes Encoding Anthocyanin Synthases from Mulberry (Morus alba)

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