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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (08): 1538-1543.doi: 10.3724/SP.J.1006.2012.01538

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Generation of Aphid Resistant Transgenic Wheat with aha From Arisaema heterophyllum by Particle Bombardment

ZHANG Yan1,YU Xiu-Dao1,TANG Ke-Xuan2,XIA Lan-Qin1,*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
  • Received:2011-12-21 Revised:2012-04-15 Online:2012-08-12 Published:2012-05-11
  • Contact: 夏兰琴, E-mail: xialq@mail.caas.net.cn, Tel: 010-82105804

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Abstract: Agglutinin is a class of mannose-binding protein, which has detrimental effect on aphids and other pests. In this study, the vector harboring aha cloned from Arisaema heterophyllum directed by rice Rubisco small subunit promoter (rbcS) was constructed. By co-transformed with pAHC20 that harbors bar selection gene, the aha gene was transferred into wheat variety Kenong 199 via bombardment. After induction, regeneration, two rounds of selection and with the conformation by PCR analysis, 42 transgenic plants with foreign aha gene were obtained, average co-transformation efficiency of 2.41%. According to PCR analysis, the segregation of the T1plants was basically consistent with Mendel’s separation. Aphid resistance bioassay was carried out using eight randomly selected transgenic lines by multiple discrimination method. One high-resistant and three low-resistant transgenic lines were identified, accounting for 44.4% of the tested materials. This study has laid a basis for application of aha gene in development of aphid resistant transgenic wheat.

Key words: Wheat, Agglutinin, rbcS promoter, Particle bombardment, Co-transformation, Insect resistance

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