Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (12): 2192-2197.doi: 10.3724/SP.J.1006.2012.02192

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analyzing Avirulence Genes of Magnaporthe oryzae from Heilongjiang Province and Screening Rice Germplasm with Resistance to Blast Fungus

LI Xiang-Xiao1,WANG Qian1,LUO Sheng-Xiang1,HE Yun-Xia2,ZHU Ling-Hua1,ZHOU Yong-Li1,*,LI Zhi-Kang1   

  1. 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China; 2 Institute of Biology Technique, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
  • Received:2012-04-06 Revised:2012-07-05 Online:2012-12-12 Published:2012-10-08

Abstract:

Theavirulence genes from 177 single spore strains of Magnaporthe grisea isolated from different rice varieties in Heilongjiang province were detected by specific primers. The results indicated that Avr-pita, ACE1, Avr-pia, Avr-pit, Avr1-co39, Avr-pik, and Avr-pizt could be detected in the strains from different rice-growing regions, and the amplification frequencies of avirurence genes were associated with the geographical orgin and rice variety, ACE1 and Avr1-co39 showed the highest and lowest frequency, that was 61.6%and 31.6%, respectively. Moreover, 20 monogenic lines with known blast resistance genes from International Rice Research Institute (IRRI) were used to analyze the genes which were valuable in breeding program in Heilongjiang Province by artificial inoculation. The resistance gene Pi-9 had the broadest resistance spectrum to theisolates from six regions.

Key words: Magnaporthe grisea, Avirulence gene, Virulence frequency, Resistance

[1]Liu J L, Wang X J, Mitchell T, Hu Y J, Liu X L, Dai L Y, Wang G L. Recent progress and understanding of the molecular mechanisms of the rice Magnaporthe oryzae interaction. Mol Plant Pathol, 2010, 11: 419–427



[2]Flor H H. Current status of the gene-for-gene concept. Annu Rev Phytopathol, 1971, 9: 275–296



[3]Lei C-L(雷财林), Wang J-L(王久林), Jiang W-R(蒋婉茹), Ling Z-Z(凌忠专). Study on pathologic races and virulence of blast fungus and their movement in japonica rice-growing region of Northern China. Acta Agron Sin (作物学报), 2000, 26(6): 769–776 (in Chinese with English abstract)



[4]Mekwatanakarn P, Kositratana W, Levy M, Zeigler R S. Pathotype and virulence gene diversity of Pyricularia grisea in Thailand as determined by rice lines near isogenic for major resistance genes. Plant Disease, 2000, 84: 60–70



[5]Sun Q(孙强), Zhang S-Y(张三元), Zhang J-G(张俊国), Yang C-G(杨春刚). Current situation of rice production in Northeast of China and counter measures. North Rice (北方水稻), 2010, 40(2): 72–74 (in Chinese with English abstract)



[6]Lei C-L(雷财林), Zhang G-M(张国民), Cheng Z-J(程治军), Ma J-T(马军滔), Wang J-L(王久林), Xin A-H(辛爱华), Chen P(陈平), Xiao J-L(肖家雷), Zhang X(张欣), Liu Y-X(刘迎雪), Guo X-P(郭秀平), Wang J(王洁), Zhai H-Q(翟虎渠), Wan J-M(万建民). Pathogenic races and virulence gene structure of Magnaporthe oryzae population and rice breeding strategy for blast resistance in Heilongjiang province. Acta Agron Sin (作物学报), 2011, 37(1): 18–27 (in Chinese with English abstract)



[7]Zhang Q-F(张崎峰), Jin X-H(靳学慧), Cai X-X(蔡鑫鑫), Gong S-Y(巩双印), Liu X-Y(刘显元). Identification of Magnaporthe oryzae avirulence gene in Heilongjiang province with SSR marker. Chin Agric Sci Bull (中国农学通报), 2010, 26(10): 250–254 (in Chinese with English abstract)



[8]Liu H-Z(刘华招), Liu Y(刘延), Liu H-L(刘化龙), Xu Z-J(徐正进), Chen W-F(陈温福). Distribution of two blast resistance genes Pib and Pita in major rice cultivars in Heilongjiang province in China. J Northeast Agric Univ (东北农业大学学报), 2011, 42(4): 27–31 (in Chinese with English abstract)



[9]Li C-Y(李成云), Luo C-X(罗朝喜), Li J-B(李进斌), Shen Y(沈瑛), Ise K(伊势一男). Mapping avirulence gene in the rice blast fungus Magnaporthe grisea. Sci Agric Sin (中国农业科学), 2000, 33(3): 49–53 (in Chinese with English abstract)



[10]Shi J(石军), Wang Z(王志), Li S-G(李仕贵), Ma B-T(马炳田). Correlation between pathotypes and DNA fingerprinting patterns based on virulence gene of rice blast fungus. Mol Plant Breed (分子植物育种), 2008, 6(6): 1050–1056 (in Chinese with English abstract)



[11]Zheng X-B(郑晓波),Wang Y-C(王源超), Chen Q-H(陈庆河), Zhang Z-G(张正光). Molecular marker methods of Magnaporthe oryzae avirulence gene. China, 200610096493.9, 2007 (in Chinese)



[12]Wang J-F(王建飞), Bao Y-M(鲍永美), Li P-F(李培富), Zhang H-S(张红生). Primary study on correlation between pathotypes and DNA fingerprintings based on avirulence gene sequence of rice blast fungus Magnaporthe oryzae. Chin J Rice Sci (中国水稻科学), 2006, 20(1): 109–112 (in Chinese with English abstract)



[13]Fukuta Y. Development of differential varieties for blast resistance in IRRI-Japan collaborative research project. In: Kawasaki S ed. Rice Blast: Interaction with Rice and Control. Netherlands: Kluwer Academic Publishers, 2004. pp 229–233



[14]Zhu X-Y(朱小源), Yang Q-Y(杨祈云), Yang J-Y(杨健源), Lei C-L(雷财林), Wang J-L(王久林), Ling Z-Z(凌忠专). Differentiation ability of monogenic lines to Magnaporthe grisea in indica rice. Acta Phytopathol Sin (植物病理学报), 2004, 34(4): 361–368 (in Chinese with English abstract)



[15]Zhou J-H(周江鸿), Wang J-L(王久林), Jiang W-R(蒋琬如), Lei C-L(雷财林), Ling Z-Z(凌忠专). Virulence genes diversity and geographic distribution of Pyricularia grisea in China. Acta Agron Sin (作物学报), 2003, 29(5): 646–651 (in Chinese with English abstract)



[16]Yu S B, Xu W J, Vijayakumar C H M, Ali J, Fu B Y, Xu J L, Jiang Y Z, Marghirang R, Domingo J, Aquino C, Virmani S S, Li Z K. Molecular diversity and multilocus organization of the parental lines used in the international rice molecular breeding program. Theor Appl Genet, 2003, 108: 131–140



[17]Valent B, Khang C H. Recent advances in rice blast effector research. Curr Opin Plant Biol, 2010, 13: 434–441



[18]Yasuda N, Noguchi M T, Fujita Y. Partial mapping of avirulence genes Avr-pii and Avr-pia in the rice blast fungus Magnaporthe oryzae. Can J Plant Pathol, 2006, 28: 494–498



[19]Farman M L, Leong S A. Genetic and physical mapping of temoleres in the rice blast fungus, Magnaporthe oryzae. Genetics, 1995, 140: 479–492



[20]Kaye C, Milazzo J, Rozenfeld S, Lebrun M H, Tharreau D. The development of simple sequence repeat markers for Magnaporthe oryzae and their integration into an established genetic linkage map. Fungal Genet Biol, 2003, 40: 207–214



[21]Zhang L-H(张连洪), Yan J-Y(燕继晔), Zhao W-S(赵文生), Zhang G-Z(张国珍), Guo L-Y(国立耘), Peng Y-L(彭友良). Mapping of avirulence gene AVR-Pi-km in Magnaporthe grisea. Acta Phytopathol Sin (植物病理学报), 2006, 36(2): 116–122 (in Chinese with English abstract)



[22]Ma J-T(马军韬), Zhang G-M(张国民), Xin A-H(辛爱华), Xiao J-L(肖家雷), Liu Y-X(刘迎雪), Gong X-J(宫秀杰). Identification and analysis of the physiologic races of Magnaporthe oryzae in Heilongjiang Province. Plant Prot (植物保护), 2010, 36(3): 97–99 (in Chinese with English abstract)

[1] TIAN Tian, CHEN Li-Juan, HE Hua-Qin. Identification of rice blast resistance candidate genes based on integrating Meta-QTL and RNA-seq analysis [J]. Acta Agronomica Sinica, 2022, 48(6): 1372-1388.
[2] ZHOU Wen-Qi, QIANG Xiao-Xia, WANG Sen, JIANG Jing-Wen, WEI Wan-Rong. Mechanism of drought and salt tolerance of OsLPL2/PIR gene in rice [J]. Acta Agronomica Sinica, 2022, 48(6): 1401-1415.
[3] DENG Zhao, JIANG Nan, FU Chen-Jian, YAN Tian-Zhe, FU Xing-Xue, HU Xiao-Chun, QIN Peng, LIU Shan-Shan, WANG Kai, YANG Yuan-Zhu. Analysis of blast resistance genes in Longliangyou and Jingliangyou hybrid rice varieties [J]. Acta Agronomica Sinica, 2022, 48(5): 1071-1080.
[4] WANG Xing-Rong, LI Yue, ZHANG Yan-Jun, LI Yong-Sheng, WANG Jun-Cheng, XU Yin-Ping, QI Xu-Sheng. Drought resistance identification and drought resistance indexes screening of Tibetan hulless barley resources at adult stage [J]. Acta Agronomica Sinica, 2022, 48(5): 1279-1287.
[5] ZHU Zheng, WANG Tian-Xing-Zi, CHEN Yue, LIU Yu-Qing, YAN Gao-Wei, XU Shan, MA Jin-Jiao, DOU Shi-Juan, LI Li-Yun, LIU Guo-Zhen. Rice transcription factor WRKY68 plays a positive role in Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2022, 48(5): 1129-1140.
[6] LIU Dan, ZHOU Cai-E, WANG Xiao-Ting, WU Qi-Meng, ZHANG Xu, WANG Qi-Lin, ZENG Qing-Dong, KANG Zhen-Sheng, HAN De-Jun, WU Jian-Hui. Rapid identification of adult plant wheat stripe rust resistance gene YrC271 using high-throughput SNP array-based bulked segregant analysis [J]. Acta Agronomica Sinica, 2022, 48(3): 553-564.
[7] YANG Xin, LIN Wen-Zhong, CHEN Si-Yuan, DU Zhen-Guo, LIN Jie, QI Jian-Min, FANG Ping-Ping, TAO Ai-Fen, ZHANG Li-Wu. Molecular identification of a geminivirus CoYVV and screening of resistant germplasms in jute [J]. Acta Agronomica Sinica, 2022, 48(3): 624-634.
[8] ZHANG Si-Meng, NI Wen-Rong, LYU Zun-Fu, LIN Yan, LIN Li-Zhuo, ZHONG Zi-Yu, CUI Peng, LU Guo-Quan. Identification and index screening of soft rot resistance at harvest stage in sweetpotato [J]. Acta Agronomica Sinica, 2021, 47(8): 1450-1459.
[9] FU Hua-Ying, ZHANG Ting, PENG Wen-Jing, DUAN Yao-Yao, XU Zhe-Xin, LIN Yi-Hua, GAO San-Ji. Identification of resistance to leaf scald in newly released sugarcane varieties at seedling stage by artificial inoculation [J]. Acta Agronomica Sinica, 2021, 47(8): 1531-1539.
[10] XI Ling, WANG Yu-Qi, ZHU Wei, WANG Yi, CHEN Guo-Yue, PU Zong-Jun, ZHOU Yong-Hong, KANG Hou-Yang. Identification of resistance to wheat and molecular detection of resistance genes to wheat stripe rust of 78 wheat cultivars (lines) in Sichuan province [J]. Acta Agronomica Sinica, 2021, 47(7): 1309-1323.
[11] ZUO Xiang-Jun, FANG Peng-Peng, LI Jia-Na, QIAN Wei, MEI Jia-Qin. Characterization of aphid-resistance of a hairy wild Brassica oleracea taxa, B. incana [J]. Acta Agronomica Sinica, 2021, 47(6): 1109-1113.
[12] MA Yan-Bin, WANG Xia, LI Huan-Li, WANG Pin, ZHANG Jian-Cheng, WEN Jin, WANG Xin-Sheng, SONG Mei-Fang, WU Xia, YANG Jian-Ping. Transformation and molecular identification of maize phytochrome A1 gene (ZmPHYA1) in cotton [J]. Acta Agronomica Sinica, 2021, 47(6): 1197-1202.
[13] ZHAO Jia-Jia, QIAO Ling, WU Bang-Bang, GE Chuan, QIAO Lin-Yi, ZHANG Shu-Wei, YAN Su-Xian, ZHENG Xing-Wei, ZHENG Jun. Seedling root characteristics and drought resistance of wheat in Shanxi province [J]. Acta Agronomica Sinica, 2021, 47(4): 714-727.
[14] ZHENG Ying-Xia, CHEN Du, WEI Peng-Cheng, LU Ping, YANG Jin-Yue, LUO Shang-Ke, YE Kai-Mei, SONG Bi. Effects of planting density on lodging resistance and grain yield of spring maize stalks in Guizhou province [J]. Acta Agronomica Sinica, 2021, 47(4): 738-751.
[15] HAN Bei, WANG Xu-Wen, LI Bao-Qi, YU Yu, TIAN Qin, YANG Xi-Yan. Association analysis of drought tolerance traits of upland cotton accessions (Gossypium hirsutum L.) [J]. Acta Agronomica Sinica, 2021, 47(3): 438-450.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!