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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (12): 2192-2197.doi: 10.3724/SP.J.1006.2012.02192

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Analyzing Avirulence Genes of Magnaporthe oryzae from Heilongjiang Province and Screening Rice Germplasm with Resistance to Blast Fungus

LI Xiang-Xiao1,WANG Qian1,LUO Sheng-Xiang1,HE Yun-Xia2,ZHU Ling-Hua1,ZHOU Yong-Li1,*,LI Zhi-Kang1   

  1. 1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China; 2 Institute of Biology Technique, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
  • Received:2012-04-06 Revised:2012-07-05 Online:2012-12-12 Published:2012-10-08

Abstract:

Theavirulence genes from 177 single spore strains of Magnaporthe grisea isolated from different rice varieties in Heilongjiang province were detected by specific primers. The results indicated that Avr-pita, ACE1, Avr-pia, Avr-pit, Avr1-co39, Avr-pik, and Avr-pizt could be detected in the strains from different rice-growing regions, and the amplification frequencies of avirurence genes were associated with the geographical orgin and rice variety, ACE1 and Avr1-co39 showed the highest and lowest frequency, that was 61.6%and 31.6%, respectively. Moreover, 20 monogenic lines with known blast resistance genes from International Rice Research Institute (IRRI) were used to analyze the genes which were valuable in breeding program in Heilongjiang Province by artificial inoculation. The resistance gene Pi-9 had the broadest resistance spectrum to theisolates from six regions.

Key words: Magnaporthe grisea, Avirulence gene, Virulence frequency, Resistance

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