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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (05): 806-815.doi: 10.3724/SP.J.1006.2013.00806

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Cloning and Transcription Function Analysis of Cotton Transcription Factor GhGT30 Gene

LI Yue1,2,3,SUN Jie1,CHEN Shou-Yi2,*,XIE Zong-Ming3,*   

  1. 1Agricultural college of Shihezi University / The Key Laboratory of Oasis Eco-Agriculture, Xinjiang Production and Construction Group, Shihezi 832003, China; 2 National Key Laboratory of Plant Genomics / Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 3 Xinjiang Academy of Agricultural and Reclamation Science / Center for Molecular Agrobiotechnology and Breeding, Shihezi 832003, China
  • Received:2012-09-25 Revised:2012-12-12 Online:2013-05-12 Published:2013-01-28
  • Contact: 谢宗铭, E-mail: xiezm2008@yahoo.com.cn; Tel: 0993-6683583; 陈受宜, E-mail: sychen@genetics.ac.cn, Tel: 010-64806621

Abstract:

Trihelix transcription factors play an important role in the regulation of plant growth and development, as well as in their response to many kinds of abiotic stress. According to expressed sequence tag (EST), full-length cDNA sequence of trihelix transcription factor was cloned from upland cotton (Gossypium hirsutum L.) using the methods of rapid-amplification of cDNA ends (RACE) and RT-PCR. Sequence analysis showed that the full-length of GhGT30 (GenBank accession No. JQ013098) was 2210 bp, containing a 2025 bp open reading frame which encoded a protein of 675 amino acids with predicted molecular weight of 76.26 kD and a isoelectric point of 6.21. SMART analysis showed GhGT30 with each trihelix domain at N-terminal and C-terminal, belonging to GT-2-type factors. Amino acid sequence alignment revealed that N-terminal of GhGT30 shared high degree of identity with other higher plant GT proteins. The phylogenetic tree showed that GhGT30 was located at the same branch with GmGT-2B. Transient expression of recombinant plasmid GhGT30/PBI221-GFP in Arabidopsis protoplasts showed that GhGT30 was located in cell nuclei. Real-time quantitative PCR (qPCR) showed that GhGT30 was expressed in a higher level in flower, fiber (12 DPA) than in root, stem leaf, and ovule (0 DPA). The gene was differentially respondes to various abiotic stresses (dehydration, high salinity, and low temperature) and ABA, indicating that it may play important roles in response of cotton plant to abiotic stresses.

Key words: Trihelix transcription factor, Cotton, Abiotic stress, GhGT30

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