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Acta Agron Sin ›› 2013, Vol. 39 ›› Issue (10): 1856-1863.doi: 10.3724/SP.J.1006.2013.01856

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Effects of Antisense Thioredoxin s on Starch Accumulation and Expressions of Enzymes Related to Starch Synthesis in Weak-gluten Wheat Cultivar Yumai 18

REN Jiang-Ping,WANG Ya-Ying,WANG Xin-Guo,WANG Na,CHEN Xin,MENG Xiao-Dan,LI Yong-Chun,YIN Jun*   

  1. Henan Agricultural University / National Engineering Research Centre for Wheat, Zhengzhou 450002, China
  • Received:2013-01-04 Revised:2013-05-25 Online:2013-10-12 Published:2013-08-01
  • Contact: 尹钧, E-mail: xmzxyj@126.com, Tel: 0371-63558203

Abstract:

To clarify the functional mechanisms of starch accumulation by the antisense-thioredoxin s (anti-Trxs) gene into wheat, we conducted a pot experiment using two transgenic wheat lines, TY18-99 and TY18-100, and their wild type “Yumai 18” from October 2007 to June 2009. The effects of the anti-Trxs on starch accumulation and key enzymes involved in starch synthesis were compared between the transgenic lines and the wild type. Results indicated that the rates of starch and amylopectin accumulations in grain were significantly higher in the transgenic lines than in the wild type. During the whole grain-filling period, activity of starch-branching enzyme (SBE) in the transgenic lines increased significantly by 49.2% compared with that in the wild type. At late grain-filling stage, activities of ADP-glucose pyrophosphorylase (AGPase) and soluble starch synthase (SSS) in the transgenic lines increased by 45.8% and 16.0%, respectively. However, activity of granule-bound starch synthase (GBSS) in the transgenic lines had no significant variation. The real time RT-PCR assay indicated that the introduction of anti-Trxs resulted in increased expressions of SSS (SSS I, SSS II, and SSS III), SBE I, and AGPase genes and reduction of GBSS I transcription. Therefore, the enhanced expressions of AGPase, SBE I, and SSS may be the main reason for the increase in starch synthesis in anti-Trxs transgenic lines.

Key words: Wheat, Anti-sense thioredoxin s gene, Starch synthesis enzymes

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