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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (04): 629-635.doi: 10.3724/SP.J.1006.2014.00629


Mappingof QTLs for OilseedGermination RateunderStresses of Salinity and Drought in Brassica napus L. Based on SNP Genetic Map

JIAN Hong-Ju**,XIAO Yang**,LI Jia-Na,MA Zhen-Zhen,WEI Li-Juan,LIU Lie-Zhao*   

  1. College of Agronomy and Biotechnology, Southwest University / Chongqing Engineering Research Center for Rapeseed, Chongqing 400716, China
  • Received:2013-06-29 Revised:2013-10-29 Online:2014-04-12 Published:2014-01-17
  • Contact: 刘列钊, E-mail: liezhao2003@126.com, Tel: 023-68250701


The objective of this study was to identify QTLs for seed germination percentage of Brassica napus under the salinity stress and drought stress using the composite interval mapping (CIM) method. The recombinant inbred lines (RIL) population derived from a cross between yellow-seeded female parent GH06 and black-seeded male parent P174 was established by selfing for nine successive generations with single seed propagating from F2. The oilseeds were dealt with NaCl (16 g L–1 solution) for salinity stress, 20% (W/W) PEG-6000 solution for drought stress. The QTLs of germination ratein two different stress conditions were detected using the SNP genetic map constructed in 2013, which contains 2795 SNP markers with the total map length of 1832.9 cM and an average distance of 0.66 cM. A total of 19 QTLs for seed germinationrate under two stresses were located onchromosomes of A01, A03, A06, A07, A09, and C06. Twelve QTLs related to salinity stress were detected, with explained phenotypic variation from 4.9% to 10.9% of, while eight QTLs related to drought stress were detected, with explained phenotypic variation from 3.8% to 6.9% of. Some QTLs located on A03 and A09 under two stresses were detected in a near region. In conclusion, (1) the seed germination percentage is a quantitative trait controlled by many minor-effect genes, and the expression of the QTL is affected by environmental factors greatly; (2) different genes are involved in the oilseed responses to the stresses of different stages.

Key words: Brassica napus, SNP, Germination rate, Salinity stress, Drought stress, QTL

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