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Acta Agron Sin ›› 2014, Vol. 40 ›› Issue (06): 994-1001.doi: 10.3724/SP.J.1006.2014.009949

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Expression of Ta6-SFT Gene in Tobacco Induced by Drought Stress

LI Shu-Jie1,LI Jing-Wen1,ZHANG Zheng-Ying2,*   

  1. 1 Institute of Biotechnology, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; 2 Scientific Research Management Office, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China
  • Received:2013-10-13 Revised:2014-03-04 Online:2014-06-12 Published:2014-04-08
  • Contact: 张正英, E-mail: Kegc8@sina.com

Abstract:

To obtain better Ta6-SFT gene expression efficiency in transgenic plants, we constructed two Ta6-SFT gene expressionvectors driven by CaMV35S promoter and rd29Apromoter, transformed them into tobacco mediated by Agrobacterium tumefaciens, respectively. Transgenic plants were confirmed by PCR, Southern blot and Northern dot blot. The two types of transgenic line were treated with 18 day drought stress, non-transgenic tobacco as a control. Expression of Ta6-SFT was detected with semi-quantitative RT-PCR at the beginning of stress and 18 days of treatment. In addition, fructan concentration and some agronomic traits such as plant height, stem diameter at half of plant height, and leaf area were analyzed at the same time. The results showed that Ta6-SFT expression level was more active and fructan content was higher under drought stress in rd29Apromoter driven transgenic lines. Agronomic traits and physiological measurement indicated that the transgenic lines carrying Ta6-SFT driven byrd29Apromoter had stronger growth vigor under the drought stress than that by CaMV35S promoter. It follows that Ta6-SFT gene inducible expression driven byrd29Apromoter could make transgenic lines have better stress tolerance.

Key words: Ta6-SFT, Fructan, Drought stress, Inducible expression at adverse environment, Constitutive expression

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