Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2016, Vol. 42 ›› Issue (03): 320-329.doi: 10.3724/SP.J.1006.2016.00320

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Distribution of Wheat–Rye Translocation Line T1BL•1RS in Wheat and Its Association with Fusarium Head Blight Resistance

LI Tao*,ZHENG Fei,QIN Sheng-Nan,LI Lei,GU Shi-Liang   

  1. Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology / Co-innovation Center for Modern Production Technology of Grain Crops / Key Laboratory of Plant Functional Genomics of Ministry of Education; Yangzhou University, Yangzhou 225009, China
  • Received:2015-09-11 Revised:2015-11-20 Online:2016-03-12 Published:2015-12-18
  • Contact: 李韬, E-mail: taoli@yzu.edu.cn
  • Supported by:

    This study was supported by the National Major Project of Breeding for New Transgenic Organisms (2012ZX08009003-004), the National Natural Science Foundation of China (31171537), and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Abstract:

The short arm of 1R chromosome (1RS) of rye carries resistant genes to stripe rust, leaf rust, stem rust, powdery mildew and aphids. To understand if 1RS also mediates resistance to wheat Fusarium head blight (FHB), we genotyped a panel of 192 wheat accessions from diverse geographic regions and a population of recombinant inbred lines (RIL) consisting of 184 lines developed from the cross of Ning 7840 and Chokwang by 1RS-specific molecular marker Xscm9 and evaluated FHB severities in three consecutive seasons in greenhouses using single floret inoculation method. The results demonstrated that 22 of 192 accessions carried a T1BL•1RS translocation, and the mean FHB severity (PSS) of varieties carrying T1BL•1RS translocation was significantly lower than that of lines without the translocation across the three experiments (P< 0.05), indicating 1RS may have a positive effect on reducing FHB severity. 1RS-specific marker Xscm9 and Genome in situ hybridization (GISH) showed Ning 7840 carries T1BL•1RS translocation. In the population of RILs, irrespective of Fhb1 locus, the mean PSS of lines with T1BL•1RS translocation was significantly lower than that of those lines without T1BL•1RS. The effects of Fhb1 and 1RS on FHB resistance were additive and the interactions between them were not significant (P = 0.48). The results of this study suggested that 1RS of rye most likely carries the genes resistant to FHB.

Key words: Wheat, Rye, T1BL•1RS translocation, Fusarium head blight

[1]Rabinovich S. Importance of wheat-rye translocations for breeding modern cultivar of Triticum aestivum L. Euphytica, 1998, 100: 323–340

[2]Mago R, Miah H, Lawrence G J, Wellings C R, Spielmeyer W, Bariana H S, McIntosh R A, Pryor A J, Ellis J G. High-resolution mapping and mutation analysis separate the rust resistance genes Sr31, Lr26 and Yr9 on the short arm of rye chromosome 1. Theor Appl Genet, 2005, 112: 41–50

[3]Singh N, Shepherd K, McIntosh R. Linkage mapping of genes for resistance to leaf, stem and stripe rusts and ω-secalins on the short arm of rye chromosome 1R. Theor Appl Genet, 1990, 80: 609–616

[4]Anderson G R, Papa D, Peng J, Tahir M, Lapitan N L. Genetic mapping of Dn7, a rye gene conferring resistance to the Russian wheat aphid in wheat. Theor Appl Genet, 2003, 107: 1297–1303

[5]Peng J, Wang H, Haley S D, Peairs F B, Lapitan N L V. Molecular mapping of the Russian wheat aphid resistance gene Dn2414 in wheat. Crop Sci, 2007, 47: 2418–2429

[6]Lu H, Rudd J C, Burd J D, Weng Y. Molecular mapping of greenbug resistance genes Gb2 and Gb6 in T1AL?1RS wheat–rye translocations. Plant Breed, 2010, 129: 472–476

[7]Mater Y, Baenziger S, Gill K, Graybosch R, Whitcher L, Baker C, Specht J, Dweikat I. Linkage mapping of powdery mildew and greenbug resistance genes on recombinant 1RS from ‘Amigo’ and ‘Kavkaz’ wheat–rye translocations of chromosome 1RS?1AL. Genome, 2004, 47: 292–298

[8]Maheepala D C, Ehdaie B, Waines J G. Yield performance of wheat isolines with different dosages of the short arm of rye chromosome 1. J Agron Crop Sci, 2015, 201: 152–160

[9]Howell T, Hale I, Jankuloski L, Bonafede M, Gilbert M, Dubcovsky J. Mapping a region within the 1RS?1BL translocation in common wheat affecting grain yield and canopy water status. Theor Appl Genet, 2014, 127: 2695–2709

[10]Ehdaie B, Whitkus R W, Waines J G. Root biomass, water-use efficiency, and performance of wheat-rye translocations of chromosomes 1 and 2 in spring bread wheat ‘Pavon’. Crop Sci, 2003, 43: 710–717

[11]Yang M Y, Ren T H, Yan B J, Li Z, Ren Z L. Diversity resistance to Puccinia striiformis f. sp. tritici in rye chromosome arm 1RS expressed in wheat. Genet Mol Res, 2014, 13: 8783–8793

[12]刘建军, 肖永贵, 程敦公, 李豪圣, 刘丽, 宋健民, 刘爱峰, 赵振东, 何中虎. 利用揉面特性鉴定小麦1BL/1RS易位系. 作物学报, 2009, 35: 79–86

Liu J J, Xiao Y G, Chen D G, Li H S, Liu L, Song J M, Liu A F, Zhao Z D, He Z H. Identification of 1BL/1RS translocation based on Mixograph parameters in common wheat. Acta Agron Sin, 2009, 35: 79–86 (in Chinese with English abstract)

[13]肖永贵, 阎俊, 何中虎, 张勇, 张晓科, 刘丽, 李天富, 曲延英, 夏先春. 1BL/1RS易位对小麦产量性状和白粉病抗性的影响及其QTL分析. 作物学报, 2006, 32: 1636–1641

Xiao Y G, Yan J, He Z H, Zhang Y, Zhang X K, Liu L, Li T F, Qu Y Y, Xia X C. Effect of 1BL.1RS translocation on yield traits and powdery mildew resistance in common wheat and QTL analysis. Acta Agron Sin, 2006, 32: 1636–1641 (in Chinese with English abstract)

[14]余利, 何方, 陈桂玲, 崔法, 亓晓蕾, 王洪钢, 李兴锋. 利用1RS特异标记和染色体原位杂交技术鉴定小麦1BL•1RS 易位系. 作物学报, 2011, 37: 563–569

Yu L, Chen G L, Cui F, Qi X L, Wang H G, Li X F. Identification of 1BL•1RS wheat–rye chromosome translocations via 1RS specific molecular markers and genomic in situ hybridization. Acta Agron Sin, 2011, 37: 563–569 (in Chinese with English abstract)

[15]Saal B, Wricke G. Development of simple sequence repeat markers in rye (Secale cereale L.). Genome, 1999, 42: 964–972

[16]Weng Y, Azhaguvel P, Devkota R N, Rudd J C. PCR-based markers for detection of different sources of 1AL?1RS and 1BL?1RS wheat–rye translocations in wheat background. Plant Breed, 2007, 126: 482–486

[17]Bai G, Shaner G. Management and resistance in wheat and barley to Fusarium head blight. Annu Rev Phytopathol, 2004, 42: 135–161

[18]曾娟, 姜玉英. 2012年我国小麦赤霉病暴发原因分析及持续监控与治理对策. 中国植保导刊, 2013, 33(4): 38–41

Zeng J, Jiang Y Y. The causal factors for the epidemics of wheat Fusarium head blight in the year of 2012 in China and the strategies for continuous monitoring and prevention. China Plant Prot, 2013, 33(4): 38–41 (in Chinese)

[19]Buerstmayr H, Ban T, Anderson J A. QTL mapping and marker-assisted selection for Fusarium head blight resistance in wheat: a review. Plant Breed, 2009, 128: 1–26

[20]Draeger R, Gosman N, Steed A, Chandler E, Thomsett M, Schondelmaier J, Buerstmayr H, Lemmens M, Schmolke M, Mesterhazy A. Identification of QTLs for resistance to Fusarium head blight, DON accumulation and associated traits in the winter wheat variety Arina. Theor Appl Genet, 2007, 115: 617–625

[21]Cuthbert P A, Somers D J, Brulé-Babel A. Mapping of Fhb2 on chromosome 6BS: a gene controlling Fusarium head blight field resistance in bread wheat (Triticum aestivum L.). Theor Appl Genet, 2007, 114: 429–437

[22]Liu S, Zhang X, Pumphrey M O, Stack R W, Gill B S, Anderson J A. Complex microcolinearity among wheat, rice, and barley revealed by fine mapping of the genomic region harboring a major QTL for resistance to Fusarium head blight in wheat. Funct Integr Genomic, 2006, 6: 83–89

[23]Ma H, Zhang K, Gao L, Bai G, Chen H, Cai Z, Lu W. Quantitative trait loci for resistance to Fusarium head blight and deoxynivalenol accumulation in Wangshuibai wheat under field conditions. Plant Pathol, 2006, 55: 739–745

[24]Zeng J, Cao W, Hucl P, Yang Y, Xue A, Chi D, Fedak G. Molecular cytogenetic analysis of wheat–Elymus repens introgression lines with resistance to Fusarium head blight. Genome, 2013, 56: 75–82

[25]Turner M K, DeHaan L R, Jin Y, Anderson J A. Wheatgrass–wheat partial amphiploids as a novel source of stem rust and Fusarium head blight resistance. Crop Sci, 2013, 53: 1994–2005

[26]Zhang X, Shen X, Hao Y, Cai J, Ohm H W, Kong L. A genetic map of Lophopyrum ponticum chromosome 7E, harboring resistance genes to Fusarium head blight and leaf rust. Theor Appl Genet, 2011, 122: 263–270

[27]Yu J B, Bai G H, Cai S B, Ban T. Marker-assisted characterization of Asian wheat lines for resistance to Fusarium head blight. Theor Appl Genet, 2006, 113: 308–320

[28]Porebski S, Bailey L G, Baum B R. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Rep, 1997, 15: 8–15

[29]Gustafson J, Dille J. Chromosome location of Oryza sativa recombination linkage groups. Proc Natl Acad Sci USA, 1992, 89: 8646–8650

[30]Liu S, Zhang X, Pumphrey M O, Stack R W, Gill B S, Anderson J A. Complex microcolinearity among wheat, rice, and barley revealed by fine mapping of the genomic region harboring a major QTL for resistance to Fusarium head blight in wheat. Funct Integr Genomic, 2006, 6: 83–89

[31]Kim W, Johnson J W, Baenziger P S, Lukaszewski A J, Gaines C S. Agronomic effect of wheat–rye translocation carrying rye chromatin (1R) from different sources. Crop Sci, 2004, 44: 1254–1258

[1] Zhai Sheng-Nan, Cao Xin-You, Li Hao-Sheng, Li Ji-Hu, Li Fa-Ji, Liu Jin-Dong, Xia Xian-Chun, Lyu Ying-Ying, Ma Rui-Feng, Wang Ying, Geng Hong-Wei, Liu Jian-Jun. Analysis of the genetic effects of allelic variation at the Pod-A1, Pod-D1, and Pod-2D loci on peroxidase activity in wheat grains [J]. Acta Agronomica Sinica, 2026, 52(6): 1593-1603.
[2] Xi Qian-Hui, Xu Zi-Yuan, Liu Meng-Meng, Wang Hong-Yi, Lang Kai-Lin, Jing Zhen-Hai, Chen Feng, Zhao Lei. Genome-wide association study and candidate gene prediction of grain copper content in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1604-1617.
[3] Mao Jia-Qi, Huang Peng-Yu, Zhao Jia-Jia, Zheng Xing-Wei, Wu Bang-Bang, Hao Yu-Qiong, Qu Fei, Liu Cheng, Ma Peng-Tao, Zheng Jun. Evaluation of powdery mildew resistance in wheat cultivars and molecular detection of resistance genes in Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(6): 1669-1681.
[4] Hu Chuan, Zhao Kai-Nan, Huang Xiu-Li, Wu Jin-Zhi, Ren Kai-Ming, Wang He-Zheng, Fu Guo-Zhan, Huang Ming, Li You-Jun. Effects of tillage methods and nitrogen rates on yield and quality of dryland wheat under one-off irrigation [J]. Acta Agronomica Sinica, 2026, 52(6): 1830-1846.
[5] Chen Xue-Yan, He Hua-Chuan, Li Zheng-Jia, Dong Xin-Pan, Li Ou-Qi, Liu Xiao-Yun, Li Dan-Ping, Chen Zhi-Wei, Liu Guo-Xia, Lyu Sheng-Yuan, Wu Yin-Ying, Zhao Zhen-Dong, Cao Xin-You, Wan He-Ping. Dynamic changes in root organic acid secretion and its transcriptional regulatory mechanisms in ‘Jimai 60’ seedlings under combined salinity-alkalinity stress in hydroponics [J]. Acta Agronomica Sinica, 2026, 52(6): 1859-1875.
[6] Gao Pei-Yang, Li Jin-Xuan, Dong Yu-Kui, Shi Yu, Zhang Zhen, Zhang Yong-Li. Response of wheat tillering and spike formation to nitrogen rate under supplementary irrigation based on soil moisture content [J]. Acta Agronomica Sinica, 2026, 52(6): 1847-1858.
[7] Zhang Xian-Feng, Guo Li-Jian, Li Kang-Chun, Kong Bin-Xue, Liu Yu-Fang, Che Zhuo, Yang De-Long. Identification of the ABHD6 gene family and development of functional markers for grain weight in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1711-1727.
[8] Wang Zhuang-Zhuang, Wu Zi-Jun, Zhang Yong-Xin, Zhang Xin-Yuan, Yuan Li-Xue, Chen Ru-Xue, Liu Shi-Ju, Duan Jian-Zhao, Feng Wei, Wang Tong-Chao, Wang Yong-Hua. Optimized water-nitrogen synergy enhances winter wheat yield and nitrogen use efficiency in clay-loam fluvo-aquic soils of southeastern Henan, China [J]. Acta Agronomica Sinica, 2026, 52(5): 1501-1521.
[9] He Wan-Long, Geng Hong-Wei, Zhang Fei-Fei, Mikereayi·Ababaikere , Luo Zi-Yang, Li Peng-Cheng, Zhou Zhao-Yu, Cheng Yu-Kun. Development of a deep learning-based image recognition system for major wheat diseases [J]. Acta Agronomica Sinica, 2026, 52(5): 1401-1417.
[10] Zhang Zhen, Feng Lian-Jie, Shi Yu, Yu Zhen-Wen, Zhang Yong-Li. Yield formation of wheat with different ear types under water-saving supplementary irrigation conditions [J]. Acta Agronomica Sinica, 2026, 52(5): 1522-1535.
[11] Hou Si-Yu, Wang Guo-Cui, Wei Jin-Gui, Xie Wei-Xin, Yin Wen, Fan Zhi-Long, Chai Qiang, Hu Fa-Long. Effects of green manure combined with chemical nitrogen fertilizer on dry matter accumulation and yield formation of wheat in arid irrigation areas of northwestern China [J]. Acta Agronomica Sinica, 2026, 52(4): 1208-1219.
[12] Shang Yun-Qiu, Zhao Zhu, Chen Huan, Ding Yong-Gang, Qiao Yu-Qiang, Li Wei, Zhang Xiang-Qian, Cao Cheng-Fu, Du Shi-Zhou. Effects of long-term tillage practices on grain-filling and yield formation in rain-fed wheat [J]. Acta Agronomica Sinica, 2026, 52(4): 1236-1250.
[13] Qiao Yu-Xin, Li Cheng-Yue, Kang Xiao-Yu, Zhang Xin-Qi, Jia Shao-Hui, Liu Qian, Cao Ya-Li, Shi Xin-Rui, Hao Xing-Yu, Li Ping. Study on the effects of long-term no-tillage straw mulching on wheat yield improvement in dryland areas based on the APSIM model [J]. Acta Agronomica Sinica, 2026, 52(4): 1181-1192.
[14] Li Can, Zhang Xi-Wei, Zhu Bo-Tao, Zhang Pei-Pei. Functional characterization of wheat GSK kinase TaSK41 and screening for interacting proteins [J]. Acta Agronomica Sinica, 2026, 52(3): 677-687.
[15] Hou Jie, Fu Duo-Duo, Wu Hai-Feng, Hao Yu-Qiong, Zheng Xing-Wei, Wu Bang-Bang, Zhou Kai, Li Xiao-Hua, Zheng Jun, Zhao Jia-Jia. Chromosome diversity and its effects in wheat landraces from Shanxi province, China [J]. Acta Agronomica Sinica, 2026, 52(3): 746-763.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!