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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (09): 1272-1279.doi: 10.3724/SP.J.1006.2017.01272


Effects of Dwarf Gene Rht_NM9 on Contents of Endogenous Hormone RegulatingPlant Height of Common Wheat

LU Yuan1,2,CUI Chao-Fan2,HU Ping2,CHEN Pei-Du2,SHEN Xue-Fang1,HAN Qing1,WANG Yi-Fa1,XING Li-Ping2,*,CAO Ai-Zhong2,*   

  1. 1 Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; 2 State Key Laboratory of Crop Genetics and Germplasm Enhancement / Cytogenetics Institute, Nanjing Agricultural University/ Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210095, China
  • Received:2016-12-16 Revised:2017-05-10 Online:2017-09-12 Published:2017-06-05
  • Contact: xing liping, E-mail: xingliping@njau.du.cn; Cao aizhong, E-mail: caoaz@njau.edu.cn
  • Supported by:

    This study was supported by the Fundamental Research Funds for the Central Universities (KYZ201601, KYYJ201602, KYZ201401).


A dwarf wheat mutant (NM9) with additional tillers and elongated spikes was obtained by treating NAU9918 seeds with ethyl methanesulfonate (EMS). The decreased plant height in NM9 was controlled by a novel dwarf gene Rht_NM9. The endogenous plant hormones play importantroles inregulating plant height of common wheat. To understand the dwarfing mechanism of Rht_NM9 and the relationship between endogenous hormone contents and plant height, measured contents of endogenous gibberellic acid (GA), auxin (IAA), abscisic acid (ABA) and zeatin riboside (ZR) in internodes of NM9 and NAU9918 at different stagesby enzyme-linked immunosorbent assays (ELISA). Our study indicated that contents of GA and ABA in NM9 were significantly higher than there in NAU9918, and ZR content in NM9 was significantly lower than that inNAU9918 atboth booting and heading stages. Nevertheless, no difference of IAA content was observed between the mutant and the wild-type. In addition,the GA/ABA ratio in internodes of the mutant was significantly higher than that of the wild-type, however, the ratios of IAA/ABA, (IAA + GA)/ABA and ZR/ABA weresignificantly lower than thereof the wild-type. All these results indicated that plant height in wheat was regulated by multiple hormones. Plant height would be inhibited with increasingcontent of endogenous ABA and decreasingratios of IAA/ABA and ZR/ABA in wheat.

Key words: Wheat, Plant height, Mutant, Endogenous hormones

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