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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (09): 1410-1414.doi: 10.3724/SP.J.1006.2017.01410

• RESEARCH NOTES • Previous Articles    

Differential Expression and Assembly Mode of Glutamine Synthetase Isoen-zymes in Different Tissues and Organs of Maize

WANG Xiao-Chun1,2,3,ZHANG Hao-Ran3,WEI Yi-Hao1,JIA Xi-Ting3,GU Ming-Xin3,MA Xin-Ming1,*   

  1. 1 Collaborative Innovation Center of Henan Grain Crops / Henan AgriculturalUniversity, Zhengzhou 450002, China; 2 State Key Laboratory of Wheat and Maize Crop Science in China / Henan AgriculturalUniversity, Zhengzhou 450002, China; 3 Department of Biochemistry, College of Life Science / Henan AgriculturalUniversity, Zhengzhou 450002, China
  • Received:2016-12-21 Revised:2017-04-20 Online:2017-09-12 Published:2017-05-08
  • Contact: Ma Xinming, E-mail: xinmingma@126.com, Tel:13937100780 E-mail:xiaochun.w@163.com
  • Supported by:

    The study was supported by the National Key Research and Development Program of China (2016YFD0300205) and State Key Laboratory of Wheat and Maize Crop Science (39990047).


Glutamine synthetase (GS) is a key enzyme in nitrogen assimilation and recycling in cereals. In this study, the expression characteristics of GS isoenzymes in different tissues and organs of maize in grain-filling period were analyzed, and the assembly of GS isoenzymes were indentified. The GS isoforms expressed differentially in different organs were shown by Western-blot obviously; GS1 with a molecular weight of about 40 kD expressed highly in all tissues, and GS1 with a molecular weight of about 39 kD was merely expressed in the node of ear position and pedical, and GS2 with a molecular weight of about 44 kD was weakly expressed in the photosynthtic tissue such as leaf. With a modified blue naive PAGE (BNE) technique and in-gel activity analysis, the size of GS holoenzyme was calibrated; combined the 2-D gel with western-blot analysis, the subunits composition of GS isoenzymes were identified. Three GS isoenzymes with different sizes were identified in maize. GS2 holoenzyme was about 460 kD and likely a decamer, GS1 holoenzyme existed two kinds of assembly state, one was about 410 kD and likely a decamer, another was about 240 kD and more likely a pentamer; therefore, the expression of GS isoenzymes exists diversity in maize.

Key words: Maize, Glutamine synthetase (GS), Expression, Blue native PAGE (BNE), Assembly

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