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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (10): 1426-1433.doi: 10.3724/SP.J.1006.2017.01426


Phenotypes and Gene Mapping of a Thermo-sensitive Yellow Leaf Mutant of Rice

ZHANG Tian-Yu1,ZHOU Chun-Lei1,LIU Xi1,SUN Ai-Ling1,CAO Peng-Hui1,Thanhliem NGUYEN1, TIAN Yun-Lu1,ZHAI Hu-Qu1,2,JIANG Ling1,*   

  1. This study was supported by the National Key Research and Development Program of China (2016YFD0100101-08), theScience and Technology Support Project of Jiangsu Province(BE2015363), and the Science and Technology Major Projects(16030701068).
  • Received:2017-03-02 Revised:2017-05-10 Online:2017-10-12 Published:2017-05-23
  • Contact: Jing Ling, E-mail:jiangling@njau.edu.cn E-mail:olytianyu@163.com
  • Supported by:

    This study was supported by the National Key Research and Development Program of China (2016YFD0100101-08), theScience and Technology Support Project of Jiangsu Province(BE2015363), and the Science and Technology Major Projects(16030701068).


Phenotypic analysis and gene mapping of rice leaf color could lay a foundation for map-based cloning of related genes and the function research of rice photosynthetic system. Leaf yellow mutant dy1 was obtained from rice cultivar “Nanjing 11”(abbreviated as NJ11) mutated by ethyl methanesulfonate (EMS). The mutant dy1 showed leaf yellowing at seedling stage and maturity stage in natural environment, with an abnormal structure of thylakoids under TEM, and significant differences in plant height, tiller number, seed setting rate and so on. The mutant dy1 showed albinism in 20°C,etiolation in 25°C andvirescence in 30°C.The rice leaf yellow mutant dy1 was controlled by a single recessive gene. F2 population was constructed by crossing the mutant with “02428”, and the mutant phenotypesof extreme individualswere selected to map gene dy1. The gene was located in the 115 kb region of the long arm of chromosome 1, and contained 16 ORFs. Sequencing analysis showed that LOC_Os01g73450controlling a uracil nucleotidekinase, with a single base substitution in the junction of the fourth intron and the fifth exon in dy1, might be a candidate gene.And the expression of genes relatedto chloroplast synthesis was significantly decreased, indicatingdy1 may involve in the chloroplast synthesis

Key words: Rice, Thermo-sensitive, Genetic analysis, Leaf color, Gene mapping

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