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Acta Agronomica Sinica ›› 2020, Vol. 46 ›› Issue (11): 1801-1809.doi: 10.3724/SP.J.1006.2020.04049

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Low concentration of vomitoxin as elicitor induced resistance of dry rot disease of potato and its mechanism

ZHAO Xiao-Can1(), XU Yong-Qing1, HE Fu-Meng1, SUN Mei-Li1, YUAN Qiang1, WANG Xue1, KONG De-Xing1, LIU Dan1, FENG Yan-Zhong2, CHEN He-Shu2, TIAN Ming2, LIU Di2, LI Feng-Lan1,*()   

  1. 1 College of Life Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
    2 Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
  • Received:2020-02-28 Accepted:2020-07-02 Online:2020-11-12 Published:2020-07-17
  • Contact: Feng-Lan LI E-mail:ZXC1656068953@163.com;lflan715@163.com
  • Supported by:
    This study was supported by the Youth Program of National Natural Science Foundation of China(31201470);the National Natural Science Foundation of China(J1210069);the Harbin Applied Science and Technology Research and Development Project(2015RAQXJ021)

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Abstract:

Dry rot disease is a fungal disease caused by Fusarium sp. infection in the process of potato cellar storage, which seriously affects the commercial value. Vomitoxin (DON), also known as deoxynivalenol, is a secondary metabolite produced by fusarium sp. in the process of infecting potato lump. Biological control is an effective method of disease control. Using biological factors as elicitors to induce plant system produce disease resistance has become a hot research topic. In this study, in order to provide theoretical basis for biological control of dry rot disease, potato tubers were treated with low concentration of DON as elicitor to determine its role in the resistance to dry rot disease and the mechanism of inducing systemic acquired resistance (SAR) of potato. DON treatment had a certain effect on the development of dry rot disease, and affected by the concentration. The treatment of potato tubers with 5 ng mL-1 DON for four hours could effectively reduce the diameter expansion of dry rot disease lesions induced by Fusarium sambucinum. Low concentration of DON increased the activities of SOD, POD, chitinase and β-1, 3-glucanase in tuber tissue, and decreased the accumulation of MDA. DON treatment increased the activities of PAL and 4CL, the key enzymes of phenylpropane metabolism, and promoted the accumulation of total phenols, flavonoids, lignin and anthocyanins. Meanwhile, the content of endogenous signal molecules SA, JA, and ET in potato tubers could be increased by using DON as elicitor, and the expression of NPR1 (regulatory gene of plant systemic resistance) was increased.

Key words: potato, dry rot disease, Fusarium sp., vomitoxin, elicitor, induced resistance

Table 1

Real time-PCR primer sequence"

目的基因
Objective gene		 引物名称
Primer name		 引物序列
Primer sequence (5°-3°)
NPR1 NPR1-F CAGTGTGCTCGCCTATTTGTAT
NPR1-R GGTGTCGCTGAAACTTGTCG
Actin Actin-F GCTTCCCGATGGTCAAGTCA
Actin-R GGATTCCAGCTGCTTCCATTC

Fig. 1

Effects of different DON concentrations on lesion diameter of potato tubers inoculated with F. sambucinum DON: vomitoxin. Different lowercase letters above the columns show significant difference (P<0.05)."

Fig. 2

Changes in the internal morphology of potato pieces after 10 days of inoculation and culture at 6 hours after DON treatment a-l represent sterile water, 1 ng mL-1 DON, 5 ng mL-1 DON, 10 ng mL-1 DON, 15 ng mL-1 DON, 20 ng mL-1 DON, 25 ng mL-1 DON, 30 ng mL-1 DON, 35 ng mL-1 DON, 40 ng mL-1 DON, 45 ng mL-1 DON, 50 ng mL-1 DON, respectively. DON: vomitoxin."

Fig. 3

Effects of different time on size of potato tuber inoculated with F. sambucinum under DON treatments DON: vomitoxin. Different lowercase letters above the columns show significant difference (P < 0.05)."

Fig. 4

Internal morphological changes of potato nuggets after 10 days of inoculation and culture after sterile water and DON treatment DON: vomitoxin."

Fig. 5

Changes of antioxidant activity in potato tuber under DON treatments DON: vomitoxin; SOD: superoxide dismutase; POD: peroxidase; MDA: malondialdehyde. *: significantly different at P < 0.05; **: significantly different at P < 0.01."

Fig. 6

Changes of defense enzyme activity in cell wall in potato tuber under DON treatments DON: vomitoxin. *: significantly different at P<0.05; **: significantly different at P<0.01."

Fig. 7

Changes of the key enzyme activity and metabolites contents in phenyl propanoid sequence in potato tuber under DON treatments DON: vomitoxin. *: significantly different at P < 0.05; **: significantly different at P < 0.01."

Fig. 8

Changes of endogenous hormone content in potato tuber under DON treatments DON: vomitoxin; SA: salicylic acid; JA: jasmonic acid; ET: ethylene. *: significantly different at P < 0.05; **: significantly different at P < 0.01."

Fig. 9

NPR1 gene relative expression in potato tuber under DON treatments DON: vomitoxin. Different lowercase letters above the columns show significant difference (P < 0.05)."

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