芸薹属同源异源六倍体(BcBcCcCcCoCo)染色体片段缺失的鉴定及其在色泽形成基因定位中的应用

doi:10.3724/SP.J.1006.2023.24287

Abstract

Abstract:

Polyploidy leads to the complexity of individual genomes and an increase of gene copies, which brings challenges to the genomic and genetic analysis. The deletion of a chromosome or chromosome fragment often leads to corresponding phenotypic changes, and the genes controlling these traits are located within the deleted fragments. According to this principle, chromosome deletion was usually used to map genes in a specific chromosome or chromosome fragment. In this study, a Brassica auto-allohexaploid (B^cB^cC^cC^cC^oC^o) was synthesized by the hybridization between B. carinata (2n = 34, B^cB^cC^cC^c) and B. oleracea (C^oC^o, 2n = 18). Two young hexaploid plants were differentiated from the callus of the same immature embryo during embryo rescue on MS medium with colchicine and hormone. However, while one plant showed purple leaves and stems and light-yellow flowers as its maternal parent B. carinata, the other plant had green leaves and stems and white flowers like the paternal parent B. oleracea. Cytological observation, fluorescence in situ hybridization (FISH), and genome re-sequencing analysis showed that a large fragment of chromosome B04 was absent in green plant. Transcriptome analysis showed DFR gene in the deletion region and MYB90 on the reference genome B03 chromosome may be the key genes controlling the purple color formation on leaves and stems, while Z-ISO and CRTISO2 were two genes within deletion region determining the formation of yellow flowers in hexaploids.

Key words: Brassica, hexaploids, chromosome deletion, anthocyanin, carotenoids

QIU Jie, WANG Tai, CAI Bo-Wei, DUAN Sheng-Xing, XU Lin-Shan, CHEN Xiao-Di, WANG Jing, GE Xian-Hong, LI Zai-Yun. Identification of chromosome deletion in synthesized Brassica auto-allohexaploids and its application in mapping genes of pigment synthesis[J].Acta Agronomica Sinica, 2023, 49(11): 2902-2912.

Figures/Tables 9

Fig. 1

Table S1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Table 1

Relative expression level of genes in anthocyanin biosynthesis in leaf of two hexaploids"

基因号 Gene ID	基因名称 Gene name	基因位置Location	基因表达量Gene expression value (FPKM)						P-adj值 P-adj value
基因号 Gene ID	基因名称 Gene name	基因位置Location	绿1 Green 1	绿2 Green 2	绿3 Green 3	紫1 Purple 1	紫2 Purple 2	紫3 Purple 3	P-adj值 P-adj value
BjuB042150	PAL1	B01	59	43	65	8	9	9	7.13E-20
Bol025522	PAL1	C04	309	235	402	686	885	1275	3.83E-24
Bol037689	PAL1	C04	389	288	495	970	1161	1770	6.05E-27
BjuB041653	PAL1	B06	149	104	199	246	324	521	6.47E-11
Bol005411	PAL2	C06	106	113	116	397	498	626	2.32E-53
Bol004610	C4H	C03	180	141	234	327	339	520	2.47E-13
Bol033347	C4H	C04	72	119	83	3	2	21	3.43E-07
BjuB026422	C4H	B01	4	2	2	181	202	218	8.87E-08
Bol026623	4CL5	C03	120	110	160	187	253	276	4.19E-12
Bol012584	4CL3	C06	294	259	302	451	507	601	3.29E-16
BjuB043976	4CL3	B03	28	9	16	96	113	156	1.62E-16
Bol043396	CHS	C09	789	301	1009	1448	1855	1824	3.57E-07
Bol034259	CHS	C03	217	70	295	2867	3304	4032	1.32E-36
BjuB012368	CHS	B05	645	410	790	1597	1797	1863	1.76E-19
BjuB012366	CHS	B05	55	28	70	122	197	157	2.22E-10
BjuB014803	CHS	B08	65	16	90	202	260	362	5.99E-10
Bol044343	CHI	C08	50	19	57	123	144	287	7.54E-11
BjuB044274	F3'H	B02	2	2	0	20	18	40	2.55E-07
Bol043829	F3'H	C09	9	14	25	614	701	1177	9.51E-88
BjuB001305	DFR	B04^*	0	0	0	682	887	827	1.60E-25
Bol042059	ANS(LDOX)	C07	89	96	90	420	474	492	4.66E-45
BjuB044852	ANS (LDOX)	B02	78	66	96	184	225	348	9.32E-33
BjuB014115	ANS (LDOX)	B05	4	10	6	474	405	424	5.45E-55
Bol014986	ANS (LDOX)	C01	10	15	11	2981	3408	3924	3.28E-210
Bol027055	UGT75C1	C08	10	17	7	2500	3037	3696	2.17E-192
Bol038805	UGT79B1	C09	0	0	2	236	245	294	7.62E-18
BjuB035464	TT19	B02	36	25	26	76	62	102	8.82E-08
BjuB013191	TT19	B05	452	383	574	1012	1158	1550	2.87E-34
Bol021325	TT19	C02	15	11	17	655	750	1075	6.61E-113
Bol019821	TT19	C09	4	4	3	533	696	884	1.44E-65
BjuB046545	MYB12	B06	8	17	12	38	46	76	1.15E-08
Bol016164	MYBL2	C06	45	39	68	445	532	343	2.83E-32
BjuB043935	MYB90 (PAP2)	B03	0	0	0	4052	4792	4563	4.12E-36
BjuB007698	MYB111	B07	15	16	14	39	47	92	1.79E-08
BjuB004115	TT8	B08	7	9	11	43	52	65	1.26E-12
Bol012528	MYB90 (PAP2)	C06	6	9	12	111	89	99	1.01E-20

Table 1

Table 2

References 38

[1]	Van De Peer Y, Mizrachi E, Marchal K. The evolutionary significance of polyploidy. Nat Rev Genet, 2017, 18: 411-424. doi: 10.1038/nrg.2017.26 pmid: 28502977
[2]	Madlung A. Polyploidy and its effect on evolutionary success: old questions revisited with new tools. Heredity, 2013, 110: 99-104. doi: 10.1038/hdy.2012.79 pmid: 23149459
[3]	Gonzalez A, Zhao M, Leavitt J M, Lloyd A M. Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings. Plant J, 2008, 53: 814-827. doi: 10.1111/tpj.2008.53.issue-5
[4]	Tanaka Y, Sasaki N, Ohmiya A. Biosynthesis of plant pigments: anthocyanins, betalains and carotenoids. Plant J, 2008, 54: 733-749. doi: 10.1111/j.1365-313X.2008.03447.x
[5]	Zhang Y, Butelli E, Martin C. Engineering anthocyanin biosynthesis in plants. Curr Opin Plant Biol, 2014, 19: 81-90. doi: 10.1016/j.pbi.2014.05.011 pmid: 24907528
[6]	宋建辉, 郭长奎, 石敏. 植物花青素生物合成及调控. 分子植物育种, 2021, 19: 3612-3620.
	Song J H, Guo C K, Shi M. Anthocyanin biosynthesis and regulation in plants. Mol Plant Breed, 2021, 19: 3612-3620 (in Chinese with English abstract).
[7]	Albert N W, Griffiths A G, Cousins G R, Verry I M, Williams W M. Anthocyanin leaf markings are regulated by a family of R2R3-MYB genes in the genus Trifolium. New Phytol, 2015, 205: 882-893. doi: 10.1111/nph.13100 pmid: 25329638
[8]	Albert N W, Lewis D H, Zhang H, Schwinn K E, Jameson P E, Davies K M. Members of an R2R3-MYB transcription factor family in Petunia are developmentally and environmentally regulated to control complex floral and vegetative pigmentation patterning. Plant J, 2011, 65: 771-784. doi: 10.1111/tpj.2011.65.issue-5
[9]	Zhao L, Gao L, Wang H, Chen X, Wang Y, Yang H, Wei C, Wan X, Xia T. The R2R3-MYB, bHLH, WD40, and related transcription factors in flavonoid biosynthesis. Funct Integr Genomics, 2013, 13: 75-98. doi: 10.1007/s10142-012-0301-4
[10]	王紫璇, 李佳佳, 于旭东, 蔡泽坪, 罗佳佳, 徐芷蕙. 高等植物类胡萝卜素生物合成研究进展. 分子植物育种, 2021, 19: 2627-2637.
	Wang Z X, Li J J, Yu X D, Cai Z P, Luo J J, Xu Z H. Research progress in carotenoid biosynthesis in higher plants. Mol Plant Breed, 2021, 19: 2627-2637 (in Chinese with English abstract).
[11]	樊宝莲, 王晓云. 转录因子调控植物类胡萝卜素合成途径的研究进展. 分子植物育种, 2021, 19: 4401-4408.
	Fan B L, Wang X Y. Research progress in regulation of carotenoid biosynthesis by transcriptional factors in plants. Mol Plant Breed, 2021, 19: 4401-4408 (in Chinese with English abstract).
[12]	Ruiz-Sola M A, Rodriguez-Concepcion M. Carotenoid biosynthesis in Arabidopsis: a colorful pathway. Arabidopsis Book, 2012, 10: e0158. doi: 10.1199/tab.0158
[13]	Zhang B, Liu C, Wang Y, Yao X, Wang F, Wu J, King G J, Liu K. Disruption of a CAROTENOID CLEAVAGE DIOXYGENASE 4gene converts flower colour from white to yellow in Brassica species. New Phytol, 2015, 206: 1513-1526. doi: 10.1111/nph.13335 pmid: 25690717
[14]	Ohmiya A, Kishimoto S, Aida R, Yoshioka S, Sumitomo K. Carotenoid cleavage dioxygenase (CmCCD4a) contributes to white color formation in chrysanthemum petals. Plant Physiol, 2006, 142: 1193-1201. pmid: 16980560
[15]	Wang X, Wang H, Wang J, Sun R, Wu J, Liu S, Bai Y, Mun J H, Bancroft I, Cheng F, Huang S, Li X, Hua W, Wang J, Wang X, Freeling M, Pires J C, Paterson A H, Chalhoub B, Wang B, Hayward A, Sharpe A G, Park B S, Weisshaar B, Liu B, Li B, Liu B, Tong C, Song C, Duran C, Peng C, Geng C, Koh C, Lin C, Edwards D, Mu D, Shen D, Soumpourou E, Li F, Fraser F, Conant G, Lassalle G, King GJ, Bonnema G, Tang H, Wang H, Belcram H, Zhou H, Hirakawa H, Abe H, Guo H, Wang H, Jin H, Parkin I A, Batley J, Kim J S, Just J, Li J, Xu J, Deng J, Kim J A, Li J, Yu J, Meng J, Wang J, Min J, Poulain J, Wang J, Hatakeyama K, Wu K, Wang L, Fang L, Trick M, Links M G, Zhao M, Jin M, Ramchiary N, Drou N, Berkman P J, Cai Q, Huang Q, Li R, Tabata S, Cheng S, Zhang S, Zhang S, Huang S, Sato S, Sun S, Kwon S J, Choi S R, Lee T H, Fan W, Zhao X, Tan X, Xu X, Wang Y, Qiu Y, Yin Y, Li Y, Du Y, Liao Y, Lim Y, Narusaka Y, Wang Y, Wang Z, Li Z, Wang Z, Xiong Z, Zhang Z. Brassica rapa genome sequencing project C. The genome of the mesopolyploid crop species Brassica rapa. Nat Genet, 2011, 43: 1035-1039. doi: 10.1038/ng.919
[16]	Liu S, Liu Y, Yang X, Tong C, Edwards D, Parkin I A, Zhao M, Ma J, Yu J, Huang S, Wang X, Wang J, Lu K, Fang Z, Bancroft I, Yang T J, Hu Q, Wang X, Yue Z, Li H, Yang L, Wu J, Zhou Q, Wang W, King G J, Pires J C, Lu C, Wu Z, Sampath P, Wang Z, Guo H, Pan S, Yang L, Min J, Zhang D, Jin D, Li W, Belcram H, Tu J, Guan M, Qi C, Du D, Li J, Jiang L, Batley J, Sharpe A G, Park B S, Ruperao P, Cheng F, Waminal N E, Huang Y, Dong C, Wang L, Li J, Hu Z, Zhuang M, Huang Y, Huang J, Shi J, Mei D, Liu J, Lee T H, Wang J, Jin H, Li Z, Li X, Zhang J, Xiao L, Zhou Y, Liu Z, Liu X, Qin R, Tang X, Liu W, Wang Y, Zhang Y, Lee J, Kim H H, Denoeud F, Xu X, Liang X, Hua W, Wang X, Wang J, Chalhoub B, Paterson A H. The Brassica oleracea genome reveals the asymmetrical evolution of polyploid genomes. Nat Commun, 2014, 5: 3930. doi: 10.1038/ncomms4930
[17]	Perumal S, Koh C S, Jin L, Buchwaldt M, Higgins E E, Zheng C, Sankoff D, Robinson S J, Kagale S, Navabi Z K, Tang L, Horner K N, He Z, Bancroft I, Chalhoub B, Sharpe A G, Parkin I A P. A high-contiguity Brassica nigra genome localizes active centromeres and defines the ancestral Brassica genome. Nat Plants, 2020, 6: 929-941. doi: 10.1038/s41477-020-0735-y
[18]	Parkin I A, Koh C, Tang H, Robinson S J, Kagale S, Clarke W E, Town C D, Nixon J, Krishnakumar V, Bidwell S L, Denoeud F, Belcram H, Links M G, Just J, Clarke C, Bender T, Huebert T, Mason A S, Pires J C, Barker G, Moore J, Walley P G, Manoli S, Batley J, Edwards D, Nelson M N, Wang X, Paterson A H, King G, Bancroft I, Chalhoub B, Sharpe A G. Transcriptome and methylome profiling reveals relics of genome dominance in the mesopolyploid Brassica oleracea. Genome Biol, 2014, 15: R77. doi: 10.1186/gb-2014-15-6-r77
[19]	Guo N, Cheng F, Wu J, Liu B, Zheng S, Liang J, Wang X. Anthocyanin biosynthetic genes in Brassica rapa. BMC Genomics, 2014, 15: 426. doi: 10.1186/1471-2164-15-426
[20]	Mushtaq M A, Pan Q, Chen D, Zhang Q, Ge X, Li Z. Comparative leaves transcriptome analysis emphasizing on accumulation of anthocyanins in Brassica: molecular regulation and potential interaction with photosynthesis. Front Plant Sci, 2016, 7: 311.
[21]	Chen D, Liu Y, Yin S, Qiu J, Jin Q, King G J, Wang J, Ge X, Li Z. Alternatively spliced BnaPAP2.A7 isoforms play opposing roles in anthocyanin biosynthesis of Brassica napus L. Front Plant Sci, 2020, 11: 983. doi: 10.3389/fpls.2020.00983
[22]	Heng S, Cheng Q, Zhang T, Liu X, Huang H, Yao P, Liu Z, Wan Z, Fu T. Fine-mapping of the BjPur gene for purple leaf color in Brassica juncea. Theor Appl Genet, 2020, 133: 2989-3000. doi: 10.1007/s00122-020-03634-9
[23]	He Q, Wu J Q, Xue Y H, Zhao W B, Li R, Zhang L G. The novel gene BrMYB2, located on chromosome A07, with a short intron 1 controls the purple-head trait of Chinese cabbage (Brassica rapa L.). Hortic Res, 2020, 7: 97. doi: 10.1038/s41438-020-0319-z
[24]	Yan C H, An G H, Zhu T, Zhang W Y, Zhang L, Peng L Y, Chen J J, Kuang H H. Independent activation of the BoMYB2 gene leading to purple traits in Brassica oleracea. Theor Appl Genets, 2019, 132: 895-906.
[25]	谭晨. 甘蓝型油菜中基因表达的剂量效应及甘蓝-黑芥附加系的创建. 华中农业大学博士学位论文, 湖北武汉, 2017.
	Tan C. The Dose-effect of Gene Expression in Brassica napus and the Establishment of Brassica napus-B. nigra Addition Lines. PhD Dissertation of Huazhong Agricultural University, Wuhan, Hubei, China, 2017 (in Chinese with English abstract).
[26]	Schelfhout C J, Snowdon R, Cowling W A, Wroth J M. A PCR based B-genome-specific marker in Brassica species. Theor Appl Genets, 2004, 109: 917-921.
[27]	Alix K, Joets J, Ryder C D, Moore J, Barker G C, Bailey J P, King G J, Heslop-Harrison J S. The CACTA transposon Bot1 played a major role in Brassica genome divergence and gene proliferation. Plant J, 2008, 56: 1030-1044. doi: 10.1111/tpj.2008.56.issue-6
[28]	Xiong Z, Pires J C. Karyotype and identification of all homoeologous chromosomes of allopolyploid Brassica napus and its diploid progenitors. Genetics, 2011, 187: 37-49. doi: 10.1534/genetics.110.122473
[29]	Martin M. Cutadapt removes adapter sequences from high- throughput sequencing reads. EMBnet J, 2011, 17: 10-12.
[30]	Bolger A M, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics, 2014, 30: 2114-2120. doi: 10.1093/bioinformatics/btu170 pmid: 24695404
[31]	Kim D, Pertea G, Trapnell C, Pimentel H, Kelley R, Salzberg S L. TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions. Genome Biol, 2013, 14: R36. doi: 10.1186/gb-2013-14-4-r36
[32]	Anders S, Pyl P T, Huber W. HTSeq: a Python framework to work with high-throughput sequencing data. Bioinformatics, 2015, 31: 166-169 doi: 10.1093/bioinformatics/btu638 pmid: 25260700
[33]	Pertea M, Kim D, Pertea G M, Leek J T, Salzberg S L. Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown. Nat Protoc, 2016, 11: 1650-1667. doi: 10.1038/nprot.2016.095 pmid: 27560171
[34]	Yoo M J, Liu X, Pires J C, Soltis P S, Soltis D E. Nonadditive gene expression in polyploids. Annu Rev Genet, 2014, 48: 485-517. doi: 10.1146/genet.2014.48.issue-1
[35]	Song Q, Chen Z J. Epigenetic and developmental regulation in plant polyploids. Curr Opin Plant Biol, 2015, 24: 101-109. doi: 10.1016/j.pbi.2015.02.007 pmid: 25765928
[36]	Birchler J A. Aneuploidy in plants and flies: the origin of studies of genomic imbalance. Semin Cell Dev Biol, 2013, 24: 315-319. doi: 10.1016/j.semcdb.2013.02.004 pmid: 23422884
[37]	Birchler J A, Veitia R A. The gene balance hypothesis: from classical genetics to modern genomics. Plant Cell, 2007, 19: 395-402. doi: 10.1105/tpc.106.049338 pmid: 17293565
[38]	李群睿. 玉米类胡萝卜素异构酶和番茄红素ε-环化酶基因的克隆与鉴定. 东北师范大学博士学位论文,吉林长春, 2010.
	Li Q R. Cloning and Identification of Genes Coding Carotenoid Isomerase and Lycopene ε-cyclase in Corn. PhD Dissertation of Northeast Normal University Library, Changchun, Jilin, China, 2010 (in Chinese with English abstract).

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引物名称Primer name	连锁群Linkage group	距离 Distance from top	正向引物序列 Forward sequence (5°-3°)	反向引物序列 Reverse sequence (5°-3°)	片段大小 Expected product size (bp)
sJ4933	J11	27.8	TGCAATCAGATCCGACTCAG	CTTGCAAATATTCAGGCCGT	335-341
sJ3891	J11	61.4	TAAACTTGTTGCAGGGGGTT	GTTTGATTGCGTGGCAGTAA	100-110
sJ3302RI	J12	10.0	AAATAACCTGCGACGGTGAC	GGGGCAAAAGGAACTTGAG	392-409
sB4817R	J12	65.8	AGCTTTTCGGTGGGTAAAGA	GCCAGTTAGCGAGTCTTTGG	250-364
sB1822	J13	17.5	TCGTTTATCCCGCGTTTATC	GCACGTTTCTTGCAGACTGA	250-267
sB1752	J13	61.5	AATGGACTCGTCCGTTATGC	TGCGTCTACCACAGACGAAG	406-422
sB2131	J14	0	GTTGCGGTCTCAAGAAGAGG	CCGCTTTAAAAATGCAGCTC	311-321
sA0306	J14	16.8	TGGGGTGGGTCTTAAATGAA	CCGTTTTAAGCCATGGAAGA	362-396
sB0372	J14	20.1	CACACTGCCATCTCTCTCCA	ACGCCGGTTGATATTAGCAC	233-245
sB2141AI	J14	26.8	CCCAAATCCGTGGCTACTTA	TATGATTGGTGGATGGCAAA	381-389
sB1935A	J14	40.4	CCTGCATGAAGGAACGTTTT	AATTCATGGAAACGGCTGTC	257-260
sJ8033	J14	47.7	TTTACGGTTTGGTTTGGCTC	GCTTGACGGCTGAAGAAAAT	150-225
sJ3874I	J15	9.9	ACAACATTTTCCATTCCCCA	ATGAGGTTTCATCCCGACAG	164-185
sB3872	J15	60.9	ACAGAAGCATTAGGCGAGGA	TCCATATAATCACCGCACGA	176-183
sJ7104	J16	35.5	TCCGCTGTCACGATTATGAA	GCTGCAGGGAAACTTGAGAC	322-337
sJ3640I	J16	82.9	CCACTCTCAATCTTCGAGCC	TGGTTGGTTTCAAGACAAAAGA	327-346
sJ13133	J17	25.8	AACCAAACCAAACCAAGTCG	GGCATCGATCAAGTGACCTT	294-300
sJ4633	J17	64.4	CCCGGAAAATAAATGAAAACAA	AACCTGAACGCCATTTTCAC	307-312
sJ34121	J18	24.8	TGCTTAAGTCCATCAATGCG	ACTGATCGAAATGGACTCGG	320-340
sB5162	J18	62.9	CACGGCTCTCAACTCTTTTG	CTGATGAGCTCGAAACCGAT	274-316

Identification of chromosome deletion in synthesized Brassica auto-allohexaploids and its application in mapping genes of pigment synthesis

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[6]	MA Chao, FENG Ya-Lan, WU Shan-Wei, ZHANG Jun, GUO Bin-Bin, XIONG Ying, LI Chun-Xia, LI You-Jun. Effects of shading at grain filling stages on anthocyanin accumulation and related gene expression characteristics in seed coat of black mung bean [J]. Acta Agronomica Sinica, 2022, 48(11): 2826-2839.
[7]	NIU Na, LIU Zhen, HUANG Peng-Xiang, ZHU Jin-Yong, LI Zhi-Tao, MA Wen-Jing, ZHANG Jun-Lian, BAI Jiang-Ping, LIU Yu-Hui. Genome-wide identification and expression analysis of potato GAUT gene family [J]. Acta Agronomica Sinica, 2021, 47(12): 2348-2361.
[8]	YANG Xiao-Meng, LI Xia, PU Xiao-Ying, DU Juan, Muhammad Kazim Ali, YANG Jia-Zhen, ZENG Ya-Wen, YANG Tao. QTL mapping for total grain anthocyanin content and 1000-kernel weight in barley recombinant inbred lines population [J]. Acta Agronomica Sinica, 2020, 46(01): 52-61.
[9]	Huan TAN,Yu-Hui LIU,Li-Xia LI,Li WANG,Yuan-Ming LI,Jun-Lian ZHANG. Cloning and Functional Analysis of R2R3 MYB Genes Involved in Anthocyanin Biosynthesis in Potato Tuber [J]. Acta Agronomica Sinica, 2018, 44(7): 1021-1031.
[10]	XU Yu-Chao,HOU Xi-Lin,XU Wei-Wei,SHEN Lu-Lu,ZHANG Shi-Lin,LIU Shi-Tuo,HU Chun-Mei. Cloning and Expression Analysis of Anthocyanidin Synthase Gene BrcANS from Purple Non-heading Chinese Cabbage [J]. Acta Agron Sin, 2016, 42(06): 850-859.
[11]	ZHOU Tian-Shan,WANG Xin-Chao,YU You-Ben,XIAO Yao,QIAN Wen-Jun,XIAO Bin,YANG Ya-Jun. Correlation Analysis between Total Catechins (or Anthocyanins) and Expression Levels of Genes Involved in Flavonoids Biosynthesis in Tea Plant with Purple Leaf [J]. Acta Agron Sin, 2016, 42(04): 525-531 .
[12]	LIU Min-Xuan,LU Ping. Distribution of Vitamin E Content and Its Correlation with Agronomic Traits and Carotenoids Content in Foxtail Millet Varieties in China [J]. Acta Agron Sin, 2013, 39(03): 398-408.
[13]	ZHANG Qiong-Yu, LI Jun, ZHAO Ai-Chun, WANG Xi-Ling, JIN Xiao-Yun, Li Zhen-Gang,YU Mao-De. Molecular Cloning and Information Analysis of ANS Genes Encoding Anthocyanin Synthases from Mulberry (Morus alba) [J]. Acta Agron Sin, 2012, 38(07): 1253-1263.
[14]	QIN Hong-Ni, YAN Meng, WANG Zhao-Hui, GUO Ying, WANG Hui, SUN Hai-Yan, LIU Zhi-Zhai, CAI Yi-Lin. QTL Mapping for Anthocyanin and Melanin Contents in Maize Kernel [J]. Acta Agron Sin, 2012, 38(02): 275-284.
[15]	WANG Hai-Wei, WANG Zhen-Lin, WANG Ping, WANG Shu-Gang, HUANG Wei, WU Yu-Guo, SUN Lan-Zhen, YIN Yan-Ping. Effect of Shading Post Anthesis on Anthocyanin Accumulation and Activities of Related Enzymes in Colored-Grain Wheat [J]. Acta Agron Sin, 2011, 37(06): 1093-1100.