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Acta Agronomica Sinica ›› 2024, Vol. 50 ›› Issue (7): 1710-1718.doi: 10.3724/SP.J.1006.2024.31060

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Construction of SNP high-density genetic map and localization of QTL for β-glucan content in oats

HAN Li1(), TANG Sheng-Sheng1, LI Jia1, HU Hai-Bin2, LIU Long-Long1,*(), WU Bin2,*()   

  1. 1Center for Agricultural Genetic, Resources Research, Shanxi Agricultural University / Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriclture and Rural Affairs, Taiyuan 030031, Shanxi, China
    2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2023-10-24 Accepted:2024-01-31 Online:2024-07-12 Published:2024-03-07
  • Contact: *E-mail: lllong781211@sina.com; E-mail: wubin03@caas.cn
  • Supported by:
    China Agriculture Research System of MOF and MARA(CARS-07-A);National Natural Science Foundation of China(30800699)

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Abstract:

β-glucan is the main functional component of oats for health care, and improving its content is of great significance to the production of high-quality oats. In this study, in order to promote the effective utilization of high β-glucan oat germplasm resources and the discovery of related genes, a genetic linkage map containing 21 linkage groups and 5032 bin markers was constructed by using resequencing technology with the 223 RIL8 populations derived from the high β-glucan variety Xiayoumai and the low β-glucan resource Chi38. The total length of the map was 2045.09 cM, and the average plot distance was 0.42 cM. The β-glucan content of RIL populations in four environments was determined by standard enzyme method and near infrared method. Combined with the determination results, QTL analysis of β-glucan content was performed by complete interval mapping method. The results showed that the β-glucan content of RIL populations was normally distributed under different environmental conditions, and there were superparent descendants. The coefficient of variation of β-glucan content in the four environments ranged from 9.06% to 16.63%. Seven QTLs related to β-glucan content in oat were detected by QTL mapping, distributed on 2D, 3D, 4C, and 4D chromosomes, with the highest contribution rate of 14.73%. The same QTL was detected in two environments with a marker interval of Chr4C_mark8361257-Chr4C_mark8384831. The results of this study provide an important theoretical basis for molecular marker-assisted breeding of oat β-glucan.

Key words: oat, SNP, genetic map, β-glucan, QTL mapping

Table 1

Statistical analysis of oat β-glucan content in parental and RIL populations"

试点
Location		 亲本 Parents RIL群体 RIL population
赤38
Chi 38 (%)		 夏莜麦
Xiayoumai (%)		 范围
Range		 平均值
Mean (%)		 变异系数
CV (%)		 偏度
Skewness		 峰度
Kurtosis		 遗传力
Heritability
北京Beijing 4.06 6.52 2.92-6.95 5.11 16.63 -0.33 -0.08 0.95
山西Shanxi 4.14 5.68 3.59-5.84 4.83 9.11 -0.07 -0.39 0.90
新疆Xinjiang 5.08 6.01 4.47-6.48 5.49 6.21 0.13 0.41 0.94
河北Hebei 4.84 5.68 4.71-7.29 5.63 9.06 0.89 0.73 0.86

Fig. 1

Frequency distribution of oat β-glucan content in RIL populations under different environments BJ, SX, XJ, and HB represent the four environments of Beijing, Shanxi, Xinjiang, and Hebei, respectively."

Table 2

Joint analysis of variance for oat β-glucan content in RIL populations in different environments"

变异来源
Source		 自由度
DF		 离均差平方和
SS		 均方
MS		 F值
F-value		 P值
P-value
基因型 Genotype 218 110.58 0.51 1.94 < 0.010
环境 Environment 3 89.06 29.69 113.57 < 0.010
基因型×环境 GE interaction 654 170.95 0.26 < 0.010
残差 Residual 216 17.76 0.08
总变异 Total 875 370.58

Table 3

Information of genetic map"

染色体
Chr.		 SNP数量
Number of
SNP		 Bin标记数量
Number of Bin
marker		 总遗传距离
Total distance
(cM)		 平均遗传距离
Average marker interval
(cM)		 最大
Max. interval
(cM)
1A 6316 304 135.06 0.44 10.26
1C 2076 79 29.91 0.38 2.43
1D 10,517 273 116.85 0.43 3.13
2A 3174 219 84.61 0.39 7.99
2C 8185 206 85.27 0.41 3.21
2D 14,888 425 158.68 0.37 4.40
3A 53,092 137 55.46 0.40 3.85
3C 22,630 231 81.24 0.35 2.65
3D 11,396 354 160.97 0.45 11.72
4A 7407 278 135.66 0.49 12.67
4C 100,146 300 120.65 0.40 12.85
4D 23,126 491 194.12 0.40 8.81
5A 141,190 85 49.91 0.59 7.92
5C 91,777 392 104.23 0.27 1.92
5D 48,610 147 52.51 0.36 1.97
6A 10,996 190 78.26 0.41 4.51
6C 25,948 302 124.40 0.41 5.25
6D 3253 165 77.76 0.47 5.78
7A 6105 161 58.28 0.36 1.98
7C 10,390 182 90.39 0.50 4.95
7D 8264 111 50.87 0.46 3.90
总计 Total 609,486 5032 2045.09 0.42 12.85

Table 4

Results of QTL localization for four environmental β-glucan contents"

地点
Location		 数量性状位点
QTL		 染色体
Chr.		 位置
Position (cM)		 标记区间
Marked interval		 加性效应
Additive
effect		 LOD值
Likelihood
of odd		 贡献率
Contribution rate (%)
北京Beijing qBG9-1 3D 73 mark52899524-mark56792010 0.1856 3.5566 7.4766
qBG12-1 4D 131.5 mark359462864-mark359731564 0.1730 2.8829 6.1583
山西Shanxi qBG9-2 3D 103 mark345485656-mark345492373 0.1406 5.0331 10.2452
qBG11-1 4C 0.5 mark8361257-mark8384831 -0.1389 4.9324 9.9790
新疆Xinjiang qBG11-1 4C 0.5 mark8361257-mark8384831 -0.1250 5.6343 14.7268
河北Hebei qBG6-1 2D 37 mark72268394-mark72566672 0.1327 3.6026 6.2789
qBG11-2 4C 115 mark704833011-mark708537606 -0.1439 2.9495 6.9865
qBG12-2 4D 38 mark40330973-mark40622203 0.1172 2.6276 4.6057

Fig. 2

Genetic linkage mapping for QTL localization of β-glucan content in oats Markers names are shown on the right of linkage groups, and their genetic positions are shown on the left (cM). LOD values are shown on the right side of QTL. The four colors in the diagram represent the four environments, and the corresponding rules are as follows: Blue: Beijing; Red: Hebei; Green: Xinjiang; Orange: Shanxi."

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