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Acta Agronomica Sinica ›› 2024, Vol. 50 ›› Issue (3): 633-644.doi: 10.3724/SP.J.1006.2024.34100

• CROP GENETICS & BREEDINGZ·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Component characterization of chromosome sets in the hybrids between sugarcane and Tripidium arundinaceum

XUE Li1(), LI Xin-Yi1, HUANG Yong-Tai1, OU Cai-Lan1, WU Xiao-Qing1, YU Ze-Huai1, CUI Ze-Tian1, ZHANG Mu-Qing1, DENG Zu-Hu2, YU Fan1,*()   

  1. 1State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources / Guangxi Key Laboratory of Sugarcane Biology / College of Agriculture, Guangxi University, Nanning 530004, Guangxi, China
    2National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
  • Received:2023-06-16 Accepted:2023-09-13 Online:2024-03-12 Published:2023-09-27
  • Contact: *E-mail: yufanky@163.com
  • Supported by:
    Sugarcane Research Foundation of Guangxi University(2022GZB006);independent fund of Guangxi Key Laboratory of sugarcane biology(GXKLSCB-20190201)

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Abstract:

T. arundinaceum (Tripidium arundinaceum) is an important wild germplasm resource of sugarcane. It is one of the important ways of sugarcane breeding to infiltrate its lineage to improve sugarcane resistance. Karyotyping of the hybrids between sugarcane and T. arundinaceum is beneficial for the efficient utilization of its various superior traits. In this study, we used species-specific primers for identifying the authenticity of the high generation hybrids between sugarcane and T. arundinaceum, and typed the sugarcane and T. arundinaceum chromosomes by fluorescence in situ hybridization to investigate the inheritance, segregation, and structural characteristics of T. arundinaceum in the offspring. These results indicated that 30 clones were true progeny with 1-10 T. arundinaceum chromosomes, indicating that their progeny population basically obeyed n+n mode of chromosome inheritance that accounted for 60% in the entire true progeny population. The probability of recombination at the chromosomal level between T. arundinaceum and sugarcane was about 16.67%, and the probability of recombination between T. arundinaceum and sugarcane of different lineages tended to be the same. Co-localization of S. spontaneum species-specific probes showed that infiltration of T. arundinaceum lineages reduced the proportion of S. officinarum and recombinant chromosomes in modern sugarcane cultivars, and simultaneously increased the proportion of S. spontaneum. In conclusion, analyzing the genetic and structural characteristics of different chromosome sets in the hybrids between sugarcane and T. arundinaceum provides a cytogenetic basis for improving the exploitation of T. arundinaceum germplasm resources in sugarcane breeding.

Key words: Saccharum, Tripidium arundinaceum, fluorescence in situ hybridization, chromosome inheritance

Fig. 1

Hybridized pedigree chart between sugarcane and T. arundinaceum"

Table 1

Experimental materials in this study"

序号
No.		 无性系
Clone		 来源
Source		 序号
No.		 无性系
Clone		 来源
Source
1 海南92-77 Hainan 92-77 T. arundinaceum 19 崖城89-59 YCE 89-59 BC4
2 拔地拉 Badila S. officinarum 20 崖城89-60 YCE 89-60 BC4
3 Np-X S. spontaneum 21 崖城89-62 YCE 89-62 BC4
4 柳城05-136 LC 05-136 S. cultivars 22 崖城89-63 YCE 89-63 BC4
5 崖城05-164 YCE 05-164 BC3 23 崖城89-66 YCE 89-66 BC4
6 崖城89-23 YCE 89-23 BC4 24 崖城89-67 YCE 89-67 BC4
7 崖城89-40 YCE 89-40 BC4 25 崖城89-70 YCE 89-70 BC4
8 崖城89-41 YCE 89-41 BC4 26 崖城89-75 YCE 89-75 BC4
9 崖城89-42 YCE 89-42 BC4 27 崖城89-76 YCE 89-76 BC4
10 崖城89-47 YCE 89-47 BC4 28 崖城89-77 YCE 89-77 BC4
11 崖城89-48 YCE 89-48 BC4 29 崖城89-79 YCE 89-79 BC4
12 崖城89-49 YCE 89-49 BC4 30 崖城89-81 YCE 89-81 BC4
13 崖城89-50 YCE 89-50 BC4 31 崖城89-85 YCE 89-85 BC4
14 崖城89-52 YCE 89-52 BC4 32 崖城89-86 YCE 89-86 BC4
15 崖城89-53 YCE 89-53 BC4 33 崖城89-88 YCE 89-88 BC4
16 崖城89-54 YCE 89-54 BC4 34 崖城89-89 YCE 89-89 BC4
17 崖城89-55 YCE 89-55 BC4 35 崖城89-90 YCE 89-90 BC4
18 崖城89-57 YCE 89-57 BC4 36 崖城89-91 YCE 89-91 BC4

Table 2

Genomic probe preparation system"

材料名称
Material name		 斑茅基因组探针
Genomic probe of T. arundinaceum (µL)		 割手密特异探针
Specific probe of S. spontaneum (µL)
10×DNA聚合酶I缓冲液 10×DNA polymerase I buffer 2.5 2.5
CY3/FITC标记的0.1 mmol L-1 dNTP混合液 CY3/FITC labeled 0.1 mmol L-1 dNTP mix 7 7
0.05 U μL-1 DNA内切酶I 0.05 U μL-1 DNase I 2 2
5 U μL-1 DNA聚合酶I 5 U μL-1 DNA polymerase I 2 2
DNA模板 DNA template 3 7.5
双蒸水 Double distilled water 8.5 4
总体系 Total system 25 25

Fig. 2

Identification of chromosomal composition of YCE 05-164 and LC 05-136 Green: T. arundinaceum genomic probe signal; Red: S. spontaneum special repetitively sequence probe signal; gray area: S. officinarum lineage; Bar: 10 μm. LC: Liucheng; YCE: Yacheng."

Fig. 3

Electrophoretic detection of PCR products of the BC4 hybrid between sugarcane and T. arundinaceum M: Standard markers; 1, 25: Hainan 92-77; 2, 26: Blank control; 3, 27: Badila; 4, 28: Np-X; 5, 29: LC 05-136; 6, 30: YCE 05-164. LC: Liucheng; YCE: Yacheng."

Fig. 4

Hybrid between sugarcane and T. arundinaceum contained six chromosomes of T. arundinaceum Red: signal of the S. spontaneum-Specific repeat probe; Green: genomic signal of T. arundinaceum; Gray area: S. officinarum lineage; Bar: 10 μm. YCE: Yacheng."

Fig. 5

Hybrid between sugarcane and T. arundinaceum contained seven chromosomes of T. arundinaceum Red: signal of the S. spontaneum-specific repeat probe; Green: genomic signal of T. arundinaceum; Gray area: S. officinarum lineage; Bar: 10 μm. YCE: Yacheng."

Fig. 6

Hybrid between sugarcane and T. arundinaceum contained eight chromosomes of T. arundinaceum Red: signal of the S. spontaneum-specific repeat probe; Green: genomic signal of T. arundinaceum; Gray area: S. officinarum lineage; Bar: 10 μm. YCE: Yacheng."

Fig. 7

Hybrid between sugarcane and T. arundinaceum contained nine chromosomes of T. arundinaceum Red: signal of the S. spontaneum-specific repeat probe; Green: genomic signal of T. arundinaceum; Gray area: S. officinarum lineage; Bar: 10 μm. YCE: Yacheng."

Fig. 8

Hybrid between sugarcane and T. arundinaceum contained zero, one, and ten T. arundinaceum chromosomes Red: signal of the S. spontaneum-specific repeat probe; Green: genomic signal of T. arundinaceum; Gray area: S. officinarum lineage; Bar: 10 μm. YCE: Yacheng."

Fig. 9

Chromosome genetic mapping of T. arundinaceum from the BC4 population between sugarcane and T. arundinaceum Red: specific probe signal of S. spontaneum; Green: genomic signal of T. arundinaceum; Blue border: translocated chromosomes with T. arundinaceum and S. officinarum; Yellow border: the recombinant chromosomes were translocated with T. arundinaceum; Red border: translocated chromosomes with T. arundinaceum and S. spontaneum. YCE: Yacheng."

Fig. 10

Chromosomal genetic characteristics of the true progenies between sugarcane and T. arundinaceum (a) the number proportion of different T. arundinaceum chromosomes in 89 combinations; (b) the ratio of normal chromosome to translocation chromosome; (c) 89 distribution of different consanguineous relationships in the combinations."

Fig. 11

Chromosomal translocations identified in population from the progeny between sugarcane and T. arundinaceum Red: specific probe signal of S. spontaneum; Green: genomic signal of T. arundinaceum; Gray area: S. officinarum lineage; Bar: 10 μm. YCE: Yacheng."

Table 3

Chromosomal composition of BC4 in the hybrid from sugarcane × T. arundinaceum"

编号
Number		 染色体数目
Number of
chromosome		 斑茅染色体
T. arundinaceum
chromosome		 割手密染色体
S. spontaneum chromosome		 甘蔗属重组
Recombination of
sugarcane chromosome		 热带种染色体
S. officinarum
chromosome
柳城05-136 LC05-136 110 0 16 30 64
崖城05-164 YCE05-164 115 15 14 12 74
崖城89-23 YCE89-23 113 7 21 19 66
崖城89-40 YCE89-40 116 9 21 16 70
崖城89-41 YCE89-41 113 7 19 20 67
崖城89-42 YCE89-42 111 7 21 12 71
崖城89-47 YCE89-47 115 8 20 16 71
崖城89-48 YCE89-48 114 8 24 18 64
崖城89-49 YCE89-49 110 9 19 15 67
崖城89-50 YCE89-50 112 10 18 17 67
崖城89-52 YCE89-52 113 9 21 17 66
崖城89-53 YCE89-53 113 6 20 18 69
崖城89-54 YCE89-54 113 8 21 20 64
崖城89-55 YCE89-55 113 9 22 18 64
崖城89-57 YCE89-57 112 7 21 16 68
崖城89-59 YCE89-59 112 7 23 20 62
崖城89-60 YCE89-60 113 8 17 14 74
崖城89-62 YCE89-62 110 7 16 16 71
崖城89-63 YCE89-63 113 8 20 15 70
崖城89-66 YCE89-66 115 0 14 16 85
崖城89-67 YCE89-67 110 7 18 16 69
崖城89-70 YCE89-70 113 7 18 14 74
崖城89-75 YCE89-75 113 6 20 20 67
崖城89-76 YCE89-76 111 7 21 18 65
崖城89-77 YCE89-77 113 9 21 15 68
崖城89-79 YCE89-79 113 5 20 14 74
崖城89-81 YCE89-81 112 1 20 19 72
崖城89-85 YCE89-85 111 7 19 14 71
崖城89-86 YCE89-86 113 8 18 15 72
崖城89-88 YCE89-88 111 8 17 19 67
崖城89-89 YCE89-89 113 9 20 16 68
崖城89-90 YCE89-90 111 8 18 16 69
崖城89-91 YCE89-91 111 6 19 13 73
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