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Acta Agron Sin ›› 1991, Vol. 17 ›› Issue (05): 340-345.

• ORIGINAL PAPERS • Previous Articles     Next Articles

Study on Plantlet Regeneration from Protoplasts of Brassicanapus

Zhou Hanyang; She Jianming; Lu Weizhong; Wu Heming   

  1. Institute of Agribiological Genetics and Physiology; Jiangsu Academy of Agricultural Sciences; Nanjing
  • Received:1991-01-12 Revised:1991-06-23 Online:1991-09-12 Published:1991-09-12

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Abstract: Protoplasts were enzymatically isolated from the hypocotyl of Brassica napus and werecultured in liquid-solid DPD medium.In 4 weeks of culture,mass of regenerated cellsand small calli were formed.The calli were placed on MS medium for proliferation.Whengrew up to 5 mm in diameter,they were transfere

Key words: Brassica napus, Hypocotyl, Protoplast culture, Plantlet regeneration

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