Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 1991, Vol. 17 ›› Issue (05): 340-345.

• ORIGINAL PAPERS • Previous Articles     Next Articles

Study on Plantlet Regeneration from Protoplasts of Brassicanapus

Zhou Hanyang; She Jianming; Lu Weizhong; Wu Heming   

  1. Institute of Agribiological Genetics and Physiology; Jiangsu Academy of Agricultural Sciences; Nanjing
  • Received:1991-01-12 Revised:1991-06-23 Online:1991-09-12 Published:1991-09-12

PDF

1138

Cited

12

Abstract: Protoplasts were enzymatically isolated from the hypocotyl of Brassica napus and werecultured in liquid-solid DPD medium.In 4 weeks of culture,mass of regenerated cellsand small calli were formed.The calli were placed on MS medium for proliferation.Whengrew up to 5 mm in diameter,they were transfere

Key words: Brassica napus, Hypocotyl, Protoplast culture, Plantlet regeneration

[1] Wang Chu-Rui, Li Kai-Xiang, Zhao Zhi, Xiao Lu, Tang Guo-Yong, Zhao Zhi-Gang, Xu Liang, Du De-Zhi, Liu Hai-Dong. Development and application of KASP markers for functional sites of the early flowering gene BnCRY2 in Brassica napus L. spring rapeseed [J]. Acta Agronomica Sinica, 2026, 52(3): 708-721.
[2] Ma Yi-Na, Wu Xiao-Ming-Yu, Li Ou-Qi, Wang Yuan, Chen Li, Zhang Ying-Chuan, Zhao Lun, Wen Jing, Fu Ting-Dong, Shen Jin-Xiong. Functional analysis of the Bna-miR1040-EIF3A module in regulating flowering time in rapeseed (Brassica napus) [J]. Acta Agronomica Sinica, 2026, 52(2): 349-362.
[3] Lou Hong-Xiang, Xing Ren-Peng, Wang Bo, Wang Jing, Xu Zheng-Hua, Zhao Jie, Kuai Jie, Zhou Guang-Sheng. Effects of sowing date on the utilization efficiency of light and temperature resources and yield in Brassica napus in the middle reaches of the Yangtze River [J]. Acta Agronomica Sinica, 2026, 52(2): 539-551.
[4] WANG Bin, MENG Jiang-Yu, QIU Hao-Liang, HE Ya-Jun, QIAN Wei. Identification and expression pattern analysis of the BnaDUF579 gene family in Brassica napus [J]. Acta Agronomica Sinica, 2025, 51(8): 2100-2110.
[5] XIA Qi, GUO Ying, WANG Kun-Mei, WANG Si-Yi, JU Jian-Ye, PENG Ya-Wen, LIU Zhong-Song, XIA Shi-Tou. Correlation between salicylic acid and anthocyanins accumulation in seeds of different varieties in Brassica napus [J]. Acta Agronomica Sinica, 2025, 51(5): 1189-1197.
[6] WANG Xiao-Lin, LIU Zhong-Song, KANG Lei, YANG Liu. Mapping of silique length and seeds per silique and transcriptome profiling of pod walls in Brassica napus L. [J]. Acta Agronomica Sinica, 2025, 51(4): 888-899.
[7] ZHANG Qin, DAI Cheng, MA Chao-Zhi. Auxin response reporter gene transformation of Brassica napus and dynamic signal analysis of GUS in different tissues [J]. Acta Agronomica Sinica, 2025, 51(3): 667-675.
[8] SUN Cheng-Ming, ZHOU Xiao-Ying, CHEN Feng, ZHANG Wei, WANG Xiao-Dong, PENG Qi, GUO Yue, GAO Jian-Qin, HU Mao-Long, FU San-Xiong, ZHANG Jie-Fu. Functional analysis and prediction of long non-coding RNA (lncRNA) in the regulation of branch angle in Brassica napus L. [J]. Acta Agronomica Sinica, 2025, 51(3): 559-567.
[9] XIE Ling-Li, LI Yong-Ling, XU Ben-Bo, ZHANG Xue-Kun. Progress on waterlogging tolerance mechanism and genetic improvement in rapeseed [J]. Acta Agronomica Sinica, 2025, 51(2): 287-300.
[10] ZHUANG Yue-Ming, SONG Yi, CHEN Hang-Hang, LU Zhi-Feng, LIAO Shi-Peng, LI Xiao-Kun, CONG Ri-Huan, REN Tao, LU Jian-Wei. Effects of application of nitrogen on endophytic microbiota and seed quality in rapeseed (Brassica napus L.) seeds [J]. Acta Agronomica Sinica, 2025, 51(12): 3357-3368.
[11] HUANG Rong, ZHOU Qu-Chen, CHEN Chu-Ming, LUO Qian, YI Dong, DU Chang-Huan, HUANG Xiang-Yu, SHENG Feng, DU Xue-Zhu. Effects of BnNRT2.3-like overexpression on nitrogen uptake, utilization efficiency, and yield in Brassica napus L. [J]. Acta Agronomica Sinica, 2025, 51(12): 3184-3197.
[12] JU Jian-Ye, YANG Liu, CHEN Hao, KANG Lei, XIA Shi-Tou, LIU Zhong-Song. Single-nucleus transcriptome analysis reveals the cellular differentiation trajectories and molecular mechanisms underlying yellow seed coat formation in rapeseed [J]. Acta Agronomica Sinica, 2025, 51(11): 2860-2874.
[13] ZHANG Wen, LI Yu, WANG Chuang, SHI Lei, DING Guang-Da. Functional study of the phosphate transporter protein BnaPT48 in Brassica napus [J]. Acta Agronomica Sinica, 2025, 51(11): 2983-2995.
[14] ZHAO Hui-Xia, GUO Yan-Li, ZHENG Yu-Ling, HE Ling, CHEN Rui, WANG Shan-Shan, ZENG Chang-Li, ZOU Jun, SHEN Jin-Xiong, FU Ting-Dong, LIU Xiao-Yun, WAN He-Ping. Differential gene expression analysis of response to alkaline salt stress in Brassica napus L. [J]. Acta Agronomica Sinica, 2025, 51(11): 3105-3118.
[15] HU Zhi-Kang, SHU Yu, WANG Hui, YANG Ying-Ying, LIAO Jun-Yu, LIU Jia, CHENG Hong-Tao, GUO Chen, ZHANG Yuan-Yuan, LIU Sheng-Yi, HU Qiong, MEI De-Sheng, LI Chao. Comprehensive evaluation of alkaline tolerance in Brassica napus at the seedling stage [J]. Acta Agronomica Sinica, 2025, 51(10): 2681-2692.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd