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Acta Agron Sin ›› 1999, Vol. 25 ›› Issue (04): 447-450.

• ORIGINAL PAPERS • Previous Articles     Next Articles

Studies on Efficient Transforming System of Oilseed Rape

Lin Liangbin;Guan Chunyun;Li Xun;Wang Guohuai;Zhou Xiaoyun;Chen Xinbo   

  1. Crop Gene Engineering Key Laboratory of Hunan Province, HNAU, Changsha, 410128
  • Received:1998-09-06 Revised:1999-01-31 Online:1999-07-12 Published:1999-07-12
  • Contact: Lin Liangbin

Abstract: Using Brassica napus cv. Xiangyou 13 as materials,BT toxic protein gene was introduced into oilseed rape via ovary-injection. Anti-kanamycin plants were obtained at highest frequency of 12.8% when 0.5~1.5μg foreign DNA was injected into the middle of ovary from 20th hour to 30th hour after pollina

Key words: Brassica napus, Genetic transformation, BT toxic protein gene, Ovary injection

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