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Acta Agron Sin ›› 2003, Vol. 29 ›› Issue (04): 486-490.

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Construction of a Brassica napus Bacterial Artificial Chromosome Library and Identification of Clones Linked to Boron Efficiency Gene

Hu Zheng;Xu Fangsen;Zhao Jianwei;Meng Jinling   

  • Received:2002-09-06 Revised:2003-01-13 Online:2003-07-12 Published:2003-07-12
  • Contact: Hu Zheng

Abstract: A bacterial artificial chromosome (BAC) library was constructed for a Brassica napus cultivar: Ning RS-1. The library contains 82944 clones with 80 kb DNA insert size in average representing equivalents of 5.1 Brassica napus haploid genome. The BAC DNA can maintain stably in E. coli after about 100

Key words: Brassica napus, BAC library, Boron efficiency gene

CLC Number: 

  • S565
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