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Acta Agron Sin ›› 2006, Vol. 32 ›› Issue (04): 630-632.

• RESEARCH NOTES • Previous Articles    

Genetic Diversity of Autotetraploid Alfalfa with Different Salt-tolerant Trait Based on SSR Marker Analysis

LIU Zhi-Peng; YANG Qing-Chuan; HU Tian-Ming; YAN Long-Feng and WANG Chao   

  1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094
  • Received:2004-11-23 Revised:1900-01-01 Online:2006-04-12 Published:2006-04-12
  • Contact: YANG Qing-Chuan

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Abstract:

With 8 SSR (simple sequence repeat) loci and 63 alleles, the genetic variation of within and between 8 alfalfa (Medicago sativa L.) populations, including 320 individuals, was analyzed. The autotetraploid method was first developed and used to analyze the allele frequencies in autotretraploid alfalfa with SSR markers. Based on the allele frequencies, the genetic heterozygosity, effective allele number, standard genetic distance, and NJ(Neighbor Jointing) dendrogram were obtained, including the high genetic diversity of within and between the 8 alfalfa populations. The genetic heterozygosities from the 0/1 method and the autotetraploid method were significantly (P<0.01), showing that the autotetraploid method is more scientific to analyze SSR polymorphism in alfalfa.

Key words: Alfalfa, SSR, Genetic diversity, Autotetraploid

CLC Number: 

  • S142
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