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Acta Agron Sin ›› 2006, Vol. 32 ›› Issue (08): 1174-1178.

• ORIGINAL PAPERS • Previous Articles     Next Articles

Influence of Introducing LPAAT and KCS Genes into Rape on Erucic Acid Content of Seed

CHEN Liu1,MAO Shan-Jing1,LU Li1,CHU Cheng-Cai2,SONNTAG Karin3,WANG You-Ping1   

  1. 1College of Bioscience and Biotechnology, Yangzhou 225009, Jiangsu, China；2 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 3 Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, Groβ Luesewitz 18190, Germany
  • Received:2005-10-11 Revised:1900-01-01 Online:2006-08-12 Published:2006-08-12
  • Contact: WANG You-Ping

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Abstract:

A protocol for Agrobacterium tumefaciens-mediated transformation of Brassica napus hypocotyls was designed. A strain with a LPAAT (lysophosphatidic acid acyltransferase) gene from Limnanthes douglasii and a KCS (beta-ketoacyl-CoA synthase) gene under control of a napin promoter was co-cultivated with hypocotyls from two genotypes of B. napus, erucic-acid-free cv. ‘Drakkar’ and high-erucic-acid cv. ‘Maplus’. In total, 247 plants were regenerated, with the regeneration percentage of 15.1% for ‘Drakkar’ and 2.0% for ‘Maplus’, respectively. Transformants were confirmed by PCR of the LPAAT gene and NAP/KCS expression cassette, and Southern blotting analysis, indicating that the foreign genes were integrated into the genome of rapeseed. The transformation frequencies of B. napus cv. ‘Drakkar’ and ‘Maplus’ were 3.6% and 0.7%, respectively. Seeds of the transformants showed a changed fatty-acid profile. The content of erucic acid was up to 10.5% in the seeds of transformants of erucic-acid-free cv. ‘Drakkar’ and increased by 5% in the seeds of transformants of cv. ‘Maplus’, compared with the control . The highest content of erucic acid in some individuals of transgenic ‘Maplus’ was 62.8%.

Key words: Brassica napus L., LPAAT, KCS, Erucic acid

CLC Number: 

  • S565
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