Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2006, Vol. 32 ›› Issue (08): 1174-1178.

• ORIGINAL PAPERS • Previous Articles     Next Articles

Influence of Introducing LPAAT and KCS Genes into Rape on Erucic Acid Content of Seed

CHEN Liu1,MAO Shan-Jing1,LU Li1,CHU Cheng-Cai2,SONNTAG Karin3,WANG You-Ping1   

  1. 1College of Bioscience and Biotechnology, Yangzhou 225009, Jiangsu, China；2 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 3 Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, Groβ Luesewitz 18190, Germany
  • Received:2005-10-11 Revised:1900-01-01 Online:2006-08-12 Published:2006-08-12
  • Contact: WANG You-Ping

PDF

1106

Cited

8

Abstract:

A protocol for Agrobacterium tumefaciens-mediated transformation of Brassica napus hypocotyls was designed. A strain with a LPAAT (lysophosphatidic acid acyltransferase) gene from Limnanthes douglasii and a KCS (beta-ketoacyl-CoA synthase) gene under control of a napin promoter was co-cultivated with hypocotyls from two genotypes of B. napus, erucic-acid-free cv. ‘Drakkar’ and high-erucic-acid cv. ‘Maplus’. In total, 247 plants were regenerated, with the regeneration percentage of 15.1% for ‘Drakkar’ and 2.0% for ‘Maplus’, respectively. Transformants were confirmed by PCR of the LPAAT gene and NAP/KCS expression cassette, and Southern blotting analysis, indicating that the foreign genes were integrated into the genome of rapeseed. The transformation frequencies of B. napus cv. ‘Drakkar’ and ‘Maplus’ were 3.6% and 0.7%, respectively. Seeds of the transformants showed a changed fatty-acid profile. The content of erucic acid was up to 10.5% in the seeds of transformants of erucic-acid-free cv. ‘Drakkar’ and increased by 5% in the seeds of transformants of cv. ‘Maplus’, compared with the control . The highest content of erucic acid in some individuals of transgenic ‘Maplus’ was 62.8%.

Key words: Brassica napus L., LPAAT, KCS, Erucic acid

CLC Number: 

  • S565
[1] WANG Wen-Xiang,HU Qiong,MEI De-Sheng,LI Yun-Chang,ZHOU Ri-Jin,WANG Hui,CHENG Hong-Tao,FU Li,LIU Jia*. Genetic Effects of Branch Angle Using Mixture Model of Major Gene Plus Polygene in Brassica napus L. [J]. Acta Agron Sin, 2016, 42(08): 1103-1111.
[2] LU Kun,QU Cun-Min,LI Sha,ZHAO Hui-Yan,WANG Rui,XU Xin-Fu,LIANG Ying,LI Jia-Na. Expression Analysis and eQTL Mapping of BnTT3 Gene in Brassica napus L. [J]. Acta Agron Sin, 2015, 41(11): 1758-1766.
[3] JIAO Cong-Cong,HUANG Ji-Xiang,WANG Yi-Long,ZHANG Xiao-Yu,XIONG Hua-Xin,NI Xi-Yuan,ZHAO Jian-Yi. Genetic Analysis of Yield-Associated Traits by Unconditional and Conditional QTL in Brassica napus [J]. Acta Agron Sin, 2015, 41(10): 1481-1489.
[4] TANG Min-Qiang,CHENG Xiao-Hui,TONG Chao-Bo,LIU Yue-Ying,ZHAO Chuan-Ji,DONG Cai-Hua,YU Jing-Yin,MA Xiao-Gen,HUANG Jun-Yan,LIU Sheng-Yi. Genome-wide Association Analysis of Plant Height in Rapeseed (Brassica napus) [J]. Acta Agron Sin, 2015, 41(07): 1121-1126.
[5] ZHANG Ya-Jie,LI Jing,PENG Hong-Kun,CHEN Xiu-Bin,ZHENG Hong-Yu,CHEN Sheng-Bei,LIU An-Guo,HU Li-Yong. Dynamic Simulation Model for Growth Duration of Rapeseed (Brassica napus) [J]. Acta Agron Sin, 2015, 41(05): 766-777.
[6] Lü Yan-Yan,FU San-Xiong,CHEN Song,ZHANG Wei,QI Cun-Kou*. Cloning of BnADH3 Gene from Brassica napus L. and Submergence Tolerance of BnADH3 Transgenic Arabidopsis [J]. Acta Agron Sin, 2015, 41(04): 565-573.
[7] ZHANG Wei-Xin,CAO Hong-Xin,ZHU Yan,LIU Yan,ZHANG Wen-Yu,CHEN Yu-Li,FU Kun-Ya. Morphological Structure Model of Leaf Space Based on Biomass at Pre-Overwintering Stage in Rapeseed (Brassica napus L.) Plant [J]. Acta Agron Sin, 2015, 41(02): 318-328.
[8] WEN Juan,XU Jian-Feng,LONG Yan,XU Hai-Ming,MENG Jin-Ling,WU Jian-Guo,SHI Chun-Hai. QTL Mapping and Analysis Based on Embryo and Maternal Genetic Systems for Semi-Essential Amino Acid Contents in Rapeseed (Brassica napus L.) [J]. Acta Agron Sin, 2015, 41(01): 57-65.
[9] JIN Yan,Lü Yan-Yan,FU San-Xiong,QI Cun-Kou. Inheritance of Major Gene Plus Polygene of Water-logging Tolerance in Brassica napus L. [J]. Acta Agron Sin, 2014, 40(11): 1964-1972.
[10] QU Cun-Min,LU Kun,LIU Shui-Yan,BU Hai-Dong,FU Fu-You,WANG Rui,XU Xin-Fu,LI Jia-Na. SNP Detection and Analysis of Genes for Flavonoid Pathway in Yellow- and Black-Seeded Brassica napus L. [J]. Acta Agron Sin, 2014, 40(11): 1914-1924.
[11] ZHOU Qing-Yuan,CUI Cui,YIN Tao,CHEN Dong-Liang,ZHANG Zheng-Sheng,LI Jia-Na. Genetic Analysis of Silique Length Using Mixture Model of Major Gene Plus Polygene in Brassica napus L. [J]. Acta Agron Sin, 2014, 40(08): 1493-1500.
[12] ZUO Qing-Song,YANG Hai-Yan,LENG Suo-Hu,CAO Shi,ZENG Jiang-Xue,WU Jiang-Sheng,ZHOU Guang-Sheng. Effects of Nitrogen Fertilizer on Nitrogen Accumulation, Translocation and Nitrogen Use Efficiency in Rapeseed (Brassica napus L.) [J]. Acta Agron Sin, 2014, 40(03): 511-518.
[13] HU Mao-Long,LONG Wei-Hua,GAO Jian-Qin,FU San-Xiong,CHEN Feng,ZHOU Xiao-Yin,PENG Qi,ZHANG Wei,PU Hui-Ming*,QI Cun-Kou,ZHANG Jie-Fu,CHEN Song. Development and Application of Allele-Specific PCR Markers for Imidazolinone-Resistant Gene BnALS1R in Brassica napus [J]. Acta Agron Sin, 2013, 39(10): 1711-1719.
[14] HUANG Hai-Tao,RONG Xiang-Min,SONG Hai-Xing,LIU Qiang,LIAO Qiong,LUO Ji-Peng,GU Ji-Dong,GUAN Chun-Yun,ZHANG Zhen-Hua. Effect of Nitrate Reductase (NR) Inhibitor on NR Activity in Oilseed Rape (Brassica napus L.) and Its Relation to Nitrate Content [J]. Acta Agron Sin, 2013, 39(09): 1668-1673.
[15] MA Zhen-Zhen,LI Jia-Na,Benjiamin WITTKOP,Martin FRAUEN,YAN Xing-Ying,LIU Lie-Zhao,XIAO Yang. Analysis of QTLs for Oil, Protein, Cellulose and Hemicellulose Contents of Seeds in Brassica napus L. [J]. Acta Agron Sin, 2013, 39(07): 1214-1222.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd