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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (01): 57-65.doi: 10.3724/SP.J.1006.2015.00057


QTL Mapping and Analysis Based on Embryo and Maternal Genetic Systems for Semi-Essential Amino Acid Contents in Rapeseed (Brassica napus L.)

WEN Juan1, XU Jian-Feng1, LONG Yan2, XU Hai-Ming1, MENG Jin-Ling2, WU Jian-Guo3,*,SHI Chun-Hai1,*   

  1. 1 College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, China; 2 College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China; 3 College of Agriculture and Food Science, Zhejiang A&F University, Lin’an 311300, China
  • Received:2014-05-09 Revised:2014-09-16 Online:2015-01-12 Published:2014-10-10
  • Contact: 石春海, E-mail: chhshi@zju.edu.cn, Tel: 0571-88982691; 吴建国, E-mail: jianguowu@zafu.edu.cn, Tel: 0571-63742133


Rapeseed meal is an important feed protein source, and the amino acid composition has a close relationship with the processing quality of feed, of which, serine, cystine and tyrosine are the semi-essential amino acids for most animals. By using newly developed two-genetic-system QTL mapping software and method for analyzing seed quality traits of dicotyledonous plants, two backcross populations from a set of doubled haploid (DH) lines derived from an elite hybrid cross between Tapidor and Ningyou 7 were used to detect the QTLs simultaneously located in the amphidiploid embryo and maternal plant nuclear genomes for the semi-essential amino acid contents of rapeseed across environments. The results showed that five QTLs for serine content, two QTLs for cystine content and five QTLs for tyrosine content were identified, which were subsequently mapped on chromosomes A1, A4, A7, A8, A9, C2, C3, or C9 and could respectively explain 59.34%, 29.66%, and 59.26% of phenotypic variation in total. Five QTLs were major effect QTLs which could explain more than 10% of phenotypic variation for each. All of these QTLs had both notable embryo and maternal additive main effects, among which three QTLs were also found to have significant QE interaction effects. One QTL cluster on chromosome A4 was discovered to contain three QTLs related to serine, cystine and tyrosine contents. Some important QTLs and the tightly linked markers will have an important application value in the later map-based cloning and marker-assisted selection.

Key words: Brassica napus L., Semi-essential acid, QTL mapping, Embryo, Maternal plant, Genetic main effect, QTL×environment (QE) interaction effect

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