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Acta Agron Sin ›› 2015, Vol. 41 ›› Issue (01): 49-56.doi: 10.3724/SP.J.1006.2015.00049

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Mapping of Powdery Mildew Resistance Gene PmCH7124 in a Putative Wheat–Thinopyrum intermedium Introgression Line

LI Jian-Bo1,QIAO Lin-Yi1,2,*,LI Xin2,ZHANG Xiao-Jun2,ZHAN Hai-Xian2,GUO Hui-Juan2,REN Yong-Kang2,CHANG Zhi-Jian2,*   

  1. 1 Graduate School of Shanxi University, Taiyuan 030006, China; 2Institute of Crop Science, Shanxi Academy of Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau of Ministry of Agriculture, Taiyuan 030031, China
  • Received:2014-06-19 Revised:2014-09-30 Online:2015-01-12 Published:2014-11-11
  • Contact: 畅志坚, E-mail: wrczj@126.com; 乔麟轶, E-mail: qiaoly1988@126.com

Abstract:

Wheat introgression line CH7124 derived from a cross between wheat-Thinopyrum intermedium line TAI8335 (resistant to powdery mildew) and common wheat variety “Jinmai 33” (susceptible to powdery mildew) exhibits immunity to Blumeria graminis f. sp. tritici (Bgt) pathotypes E09, E20, E21, E23, E26, Bg1, and Bg2 at the seedling stage. The Bgt resistance in CH7124 is similar to that in TAI8335 and its wild parent Th. intermedium. However, no Th. intermedium chromatinhas been detected according to genome in situ hybridization (GISH) assay. In this study, we determined the single dominant Bgt resistance gene in CH7124, tentatively designated PmCH7124, using populations derived from SY95-71/CH7124 (F6) and CH7124/Mianyang 11 (F1 and F2). Five SSR markers (Xgwm47, Xgwm120, Xwmc332, Xgwm501, and Xbarc101) were identified to be codominant with PmCH7124 according to bulked segregant analysis, and the closely flanking markers were Xgwm501 and Xbacr101 with genetic distances of 1.7 cM and 4.5 cM, respectively. The target resistance gene was chromosomally located on 2BL with Chinese Spring nulli-tetrasomicand ditelosomic lines. We primarily consider that PmCH7124 is a new Bgt resistance gene because its resistance spectrum, origin, chromosomal location, and linked markers are different from those of the known Bgt resistance genes, such as Pm6, Pm33, PmJM22, MlZec1, MlAB10, and MlLX99

Key words: Wheat–Th. intermedium introgression line, Resistance to powdery mildew, GISH, SSR markers, Linkage map

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