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Acta Agron Sin ›› 2006, Vol. 32 ›› Issue (08): 1248-1251.

• RESEARCH NOTES • Previous Articles     Next Articles

LRP Transgenic Indica Rice Restorer Line without Resistance Selection Marker

TANG Li1,LIU Qiao-Quan2 3,DENG Xiao-Xiang1,WU Xiao-Jin1,Samuel S M Sun2   

  1. 1Hunan Hybrid Rice Research Center, Changsha 410125, Hunan; 2Department of Biology, Chinese University of Hong Kong, New Territory, Hong Kong; 3College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, Jiangsu, China
  • Received:2005-07-29 Revised:1900-01-01 Online:2006-08-12 Published:2006-08-12

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Abstract:

The gene encoding a lysine-rich protein from the edible winged bean has been cloned and transferred into R893 and a transgenic line C28 has been generated, which expresses the lysine rich protein well and is resistance selectable marker-free. The transgenic R line or hybrid is numerically higher than the normal ones in lysine content, which is 3.752 mg/g for C28, 3.503 mg/g for R893, 3.338 for Zhun-S/C28 and 3.175 for Zhun-S/R893, respectively. The transgenic R line is numerically lower than the normal in seed setting rate, which is 76.37% for C28 and 78.56% for R893, while the transgenic hybrid is highly significantly lower than the normal, which is 76.63% for Zhun-S/C28 and 89.35% for Zhun-S/R893.

Key words: Oryza sativa L., Restorer, Transformation, Lysine content, Selectable marker-free

CLC Number: 

  • S511
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