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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (5): 892-898.doi: 10.3724/SP.J.1006.2009.00892


Fertilization Process in Flax and Its Performance Time for Each Stage

YANG Hong1,SHEN Jia-Heng1,*,LI Wei1,DING Jian-Ting1,KANG Qing-Hua2   

  1. 1College of Life Science and Technology,Harbin Normal University,Harbin 150080,China;2Institute of Economic Crop,Heilongjiang Academy of Agricultural Sciences,Harbin 150080,China
  • Received:2008-06-17 Revised:2008-12-15 Online:2009-05-12 Published:2009-03-23
  • Contact: SHEN Jia-Heng,0451-88068652 E-mail:hsdshenjiaheng@yahoo.com.cn


The experiment was conducted by means of routine paraffin-embedded sectioning technique to offer some information for its genetic breeding, gene engineering and reproductive biology. The results showed that pollen grains germinated immediately when they were fallen on stigma in self-pollinated flax, then pollen tubes grew in the intercellular space of transmitting tissue of style, entering ovary and growing on the surface of endocuticle of ovary wall, and passing micropore through placenta and funicle. Pollen tube entered one synergid, and released two sperms between egg and central cells. Sperm nucleus and cytoplasm body formed by shucking cytoplasm from sperm. One sperm nucleus entered egg, and another entered the central cell. Fusion of sperm nucleus and egg nucleus was a little slower than that of sperm nucleus and secondary nucleus. Fusion process of male and female nucleoli was observed. The performance time was as follows: Pollen grains germinated very soon on stigma. Pollen tube grew into micropyle about at 4.5 hours after flowering. Pollen tube entered one synergid and released two sperms during about 4.5–5.5 hours after flowering. Fusion of egg-sperm took place in about 5.5–6.5 hours after flowering as well as that of sperm-secondary. Primary endosperm nucleus divided at about 7.5–8.5 hours after flowering. Zygote divided at 12.5 hours after flowering. The dormancy stage of zygote was at about 6.5–12.5 hours after flowering.

Key words: Linum usitatissimum L., Pathway of pollen tube growth, Fertilization, Duration

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