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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (5): 884-891.doi: 10.3724/SP.J.1006.2009.00884


Variation and Distribution of Seed Storage Protein Content and Composition among Different Rice Varieties

ZHOU Li-Hui,LIU Qiao-Quan,ZHANG Chang-Quan,XU Yong,TANG Su-Zhu,GU Ming-Hong   

  1. Key Laboratory of Plant Functional Genomics of Ministry of Education/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province,Agricultural College,Yangzhou University,Yangzhou225009,China
  • Received:2008-09-01 Revised:2009-02-18 Online:2009-05-12 Published:2009-03-23
  • Contact: GU Ming-Hong,gumh@yzu.edu.cn


The crude protein contents (PC) in 351 varieties were measured by near infrared spectroscopy (NIRS) and their distribution and classification were analyzed. The results showed that the average value of crude protein content in indica and japonica types were 13.2% and 12.2%, respectively, with an average of 12.42%. The range of those above was 10.816.8%, 9.317.7%, and 9.317.7%, respectively. It elucidated that PC was higher in indica rice than in japonica rice. The huge difference in ratios of varieties (lines) based on their PC showed the genetic disequilibrium between subspecies indica and japonica, for exsample, the ratio of japonica rice with low PC was eight times that of indica rice with low PC. According to the criterion classifying varieties with different protein contents, most of rice genotypes fell into the group with intermediate PC, and there was very small number of varieties with either high or low PC, especially with very high PC in japonica rice. However, we could find some extreme individuals which PC were very high/low, such as those with high PC: forage rice, early maturity varieties and indica-japonica hybrid progenies close to indica in the subspecies of indica, or close to japonica in the subspecies of japonica; and those with low PC: some japonica rice (but the PC not low enough), some overseas germplasms in indica. Thus it was not impossible to find out extreme germplasms on PC from landrace, overseas germplasms or india-japonica hybrid progenies etc., which are fine basic materials in genetic and breeding researchs. From the results of SDS-PAGE analysis of the total seed storage proteins among some representative varieties, we could know that the seed storage protein composition was different among different types of rice genotypes.

Key words: Rice(Oryza sativa L.), Seed crude protein content, Seed storage proteins, Variation, Distribution

[1] Juliano B O ed. Rice Chemistry and Technology, 2nd edn. Minnesota USA: American Association of Cereal Chemists Inc. 1985. pp 1–174

[2] Liu Q-Q(刘巧泉). Genetically engineering rice for increased lysine. PhD Dissertation of Yangzhou University, 2002 (in Chinese with English abstract)

[3] Gomez K A. Effect of environment on protein and amylose content of rice. In: Chemical aspects of rice grain quality. IRRI, 1979. pp 59–68

[4] Webb B D, Bollich C N. Characteristics of rice varieties in the US department of agriculture collection. Crop Sci, 1968, 8: 361–365

[5] Juliano B O, Perez C M, Gomez K A. Variability in pretion content of rice. Kalikasan, 1972, 1: 74–81

[6] Zhang R-P(张瑞品), Xie Y-F(谢岳峰). Breeding of rice quality (水稻品质育种). In: Liu H L(刘后利) ed. Breeding of Crop Quality (农作物品质育种), Wuhan: Hubei Science & Technology Press, 2001.pp 22–99 (in Chinese)

[7] Chen N(陈能), Luo Y-K(罗玉坤), Xie L-H(谢黎虹), Zhu Z-W(朱智伟), Duan B-W(段彬伍), Zhang L-P(章林平). Protein content and its correlation with other quality parameters of rice in China. Acta Agron Sin (作物学报), 2006, 32(8): 1193–1196 (in Chinese with English abstract)

[8] Liu J-X(刘建学), Wu S-Y(吴守一). Rapid measurement of rice protein content by near infrared spectroscopy. Trans Chin Soc Agric Machinery (农业机械学报), 2001, 32(3): 68–70 (in Chinese with English abstract)

[9] Siesler H W. Near infrared reflectance spectroscopy (NIRS): A method of rational multicomponent analysis. Mikrochimica Acta, 1998, 1: 117–120

[10] John S S, Mark O W. Accuacy of NIRS instruments to analyze forage and grain. Crop Sci, 1985, 25: 1120–1122

[11] AOAC, Association of Official Analytical Chemists Official Methods of Analysis, 16th ed. Method 990.03, The Association, Washington, DC. 1995. pp 35–43

[12] Zhou L-H(周丽慧), Liu Q-Q(刘巧泉), Zhang C-Q(张昌泉), Xu Y(徐勇), Tang S-Z(汤述翥), Gu M-H(顾铭洪). The crude protein contents in rice grain measured by two different methods and their relationship, J Yangzhou Univ (Agri & Life Sci Edn) (扬州大学学报·农业与生命科学版), 2009, 30(1) (in press) (in Chinese with English abstract)

[13] Yamagata H, Sugimoto T, Tanaka K, Kasai Z. Biosynthesis of storage proteins in developing rice seeds. Plant Physiol, 1982, 70: 1094–1100

[14] Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press, 1989

[15] Kubo T. Development of low-glutelin rice by Agrobacterium-mediated genetic transformation with an antisense gene construct. In: Joint FAO/WHO Expert Consultation on Foods Derived from Biotechnology, Geneva (Switzerland), 29 May–2 June, 2000; FAO, Rome (Italy) and WHO, Geneva (Switzerland), 2000. pp 1–5

[16] Gomez K A, De Datta S K. Influence of environment on protein content of rice. Agron J, 1975, 67:565–568

[17] Liu Q-Q(刘巧泉), Zhou L-H(周丽慧), Wang H-M(王红梅), Gu M-H(顾铭洪). Advances on biosynthesis of rice seed storage proteins in molecular biology. Mol Plant Breed (分子植物育种), 2008, 6(1): 1–15 (in Chinese with English abstract)

[18] Kumamaru T, Satoh H, Iwata N, Omura T, Ogawa M, Tanaka K. Mutants for rice storage proteins. Theor Appl Genet, 1988, 76: 11–16

[19] Kusaba M, Miyahara K, Iida S, Fukuoka H, Takano T, Sassa H, Nishimura M, Nishio T. Low glutelin content1: a dominant mutation that suppresses the Glutelin multigene family via RNA silencing in rice. Plant Cell, 2003, 15: 1455–1467

[20] Iida S, Amano E, Nishio T. A rice (Oryza sativa L.) mutant having a low content of glutelin and a high content of prolamine. Theor Appl Genet, 1993, 87: 374–378

[21] Iida S, Kusaba M, Nishio T. Mutants lacking glutelin subunits in rice: Mapping and combination of mutated glutelin genes. Theor Appl Genet, 1997, 94: 177–183

[22] Lu C(卢诚), Pan X-G(潘熙淦). Inheritance of wide compatibility in rice cultivars 02428 and 8504. Chin J Rice Sci (中国水稻科学), 1992, 6(3): 113–118 (in Chinese with English abstract)

[23] Sano Y, Makekawa M, Kikuchi H. Temperature effects on Wx protein level and amylose content in the endosperm of rice. Heredity, 1985, 76: 221–222

[24] Sano Y, Katsumata M, Okuno K. Genetic studies of speciation in cultivated rice: 5. Inter- and intra-specific differentiation in the waxy gene expression of rice. Euphytica, 1986, 35: l–9

[25] Shu Q-Y(舒庆尧), Wu D-X(吴殿星), Xia Y-W(夏英武), Gao M-W(高明蔚). Microsatellites polymorphism on the waxy gene locus and their relationship to armylose content in indica and japonica rice, Oryza sativa L. Acta Genet Sin (遗传学报), 1999, 26(4): 350–358 (in Chinese with English abstract)

[26] Jin W D, Li N, Hong D L. Genetic diversity of seed storage proteins in different ecotype varieties of japonica rice and its application. Rice Sci, 2006, 13(2): 85–92

[27] Cai X L, Wang Z Y, Xing Y Y, Zhang J L, Hong M M. Aberrant splicing of intron 1 leads to the heterogeneous 5’UTR and decreased expression of waxy gene in rice cuhivars of intermediate amylose content. Plant J, 1998, 14: 459–465

[28] Bligh H F J, Larkin P D, Roach P S, Jones C A, Fu H, Park W D. Use of alternate splice sites in granule bound starch synthase mRNA from low amylose rice varieties. Plant Mol Biol, 1998, 38: 407–415

[29] Isshiki M, Morino K, Nakajima M, Okagaki R J, Wessler S R, Izawa T, Shimamoto K. A naturally occurring functional allele of the rice waxy locus has a GT to TT mutation at the 5' splice site of the first intron. Plant J, 1998, 15: 133–138

[30] Hirano H Y, Eiguchi M, Sano Y. A single base change altered the regulation of the waxy gene at post-transcriptional level during the domestication of rice. Mol Biol Evol, 1998, 15: 978–987

[31] Cai X-L(蔡秀玲), Liu Q-Q(刘巧泉), Tang S-Z(汤述翥), Gu M-H(顾铭洪), Wang Z-Y(王宗阳). Development of a molecular marker for screening the rice cultivars with intermediate amylose content in rice. J Plant Physiol Mol Biol (植物生理与分子生物学学报), 2002, 28(2): 137–144 (in Chinese with English abstract)
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