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Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (8): 1500-1507.doi: 10.3724/SP.J.1006.2009.01500

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Characteristics of Photosynthesis in Wheat Cultivars with Different Sensitivities to Ozone under O3-Free Air Concentration Enrichment Conditions

CAO Ji-Ling1,2,WANG Liang1,2,ZENG Qing1,*,LIANG Jing1,2,TANG Hao-Ye1,XIE Zu-Bin1,LIU Gang1,ZHU Jian-Guo1,Kazhuhiko KOBAYASHI3   

  1. 1 State Key Laboratory of Soil and Stainable Agricultural, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; 2 Graduate School of Chinese Academy of Sciences, Beijing 100080, China; 3 Department of Global Agricultural Science, Graduate School of Agricultural and Life Science, University of Tokyo, Tokyo 113-8657, Japan
  • Received:2008-12-01 Revised:2009-04-19 Online:2009-08-12 Published:2009-06-10
  • Contact: ZENG Qing,E-mail: qzeng@issas.ac.cn;ZHU Jian-Guo,E-mail: jgzhu@issas.ac.cn

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Abstract:

Ozone (O3) has been recognized as a secondary phytotoxic air pullutant of serious concern in agriculture because of its effects on the physiology, growth, and yield of plants. Although the effects of elevated O3 concentration on plant photosynthesis have widely studied with environment-controlled chambers, the conclusions are not certain due to the difference between free-air field and environment-controlled chambers. O3-Free Air Concentration Enrichment (O3FACE) can provide undisturbed field conditions and more reliable measurements in comparison with environment-controlled facilities. In this study, two winter wheat (Tritcium aestivum L.) cultivars (Yannong 19 and Yangmai 16) with different sensitivities to O3 were used to investigate the responses of photosynthsis characteristics to elevated O3 concentration with the Chinese O3FACE platform. Under O3 treatment for 75 d, the net photosynthetic rate (Pn), stomtal conductance (Gs), and transpiration rate (Tr) decreased significantly in both cultivars, whereas the intercellular CO2 concentration (Ci) changed slightly. This indicated that the reduction of Pnresulted from stomatal and nonstomal factors with the major role of nonstomatal. The O3-sensitive cultivar Yannong 19 showed larger reductions in Pn (61.1%), Gs (68.0%), and Tr (57.4%) than Yangmai 16 (27.9%, 37.5%, and 27.9%, respectively). In the chlorophyll fluorescence parameters, the maximal photochemical efficiency of PSII in the dark (Fv/Fm), the potential activity of PSII (Fv/Fo), the photochemical quenching (qP), and the rate of photochemical reaction (Prate) decreased in O3 treatment, but the nonphotochemical quenching (NPQ) and the rate of thermal dissipation (Drate) showed the upward tendency. The change tendency of total soluble protein content and the amount of Rubisco was similar to that of chlorophyll fluorescence parameters and Pn. The results implied that the major nonstomal factors responsible for Pn decrease under elevated O3 concentration were the carboxylation limitation of RuBP and the damage of PSII. The change extents of all parameters were larger in Yannong 19 than in Yangmai 16. The high Tr value and slow reduction of Rubisco amount in Yangmai 16 are probably crucial reasons for its high photosynthetic rate.

Key words: Ozone, Wheat, Photosynthesis, Chlorophyll fluorescence, Soluble protein, Rubisco

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