Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (8): 1546-1551.doi: 10.3724/SP.J.1006.2009.01546

• TILLAGE & CULTIVATION · PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Heterosis of Water Uptake Ability by Roots of Maize at Cell Level

LIU Xiao-Fang1,2,3, ZHANG Sui-Qi1,2,*, YANG Xiao-Qing1,2,3, and SHAN Lun1,2   

  1. 1 State Key Laboratory of Soil Erosion and Dryland Farming of the Loess Plateau, Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling 712100, China; 2 Northwest A&F University, Yangling 712100, China; 3 Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
  • Received:2008-12-30 Revised:2009-03-18 Online:2009-08-12 Published:2009-06-11
  • Contact: ZHANG Sui-Qi, E-mail: sqzhang@ms.iswc.ac.cn

PDF

1824

Cited

11

Abstract:

Cell pressure probe technique has been extensively used to study plant water relations at cell level. Effect of numerous internal or external factors on water uptake by plant root cells has been studied. However, the feasibility of this technique for investigating the difference of water related parameters among varieties of the same plant species has not been reported. In this paper, the cell pressure probe was employed to study cortex cell water related parameters of primary roots among three different maize genotypes (F1 hybrid of HD4, its female parent T4 and male parent 478) under normal and water stress conditions. The water stress was simulated with PEG-6000 with ψs value of –0.2 MPa. As to the size of root cortex cells, the diameter, length and volume of cells represented as F1 > female parent > male parent under both conditions, and there was significant differences of volume among the three maize genotypes. Water stress reduced cell diameter and length obviously so as to decreased volume significantly. The root cortex cell turgor of the three maize genotypes was approximately 0.6 MPa and the genotypic difference was not noticeable under normal culture condition. By contrast to normal condition, cell turgor values of the three maize genotypes were decreased by 0.2–0.3 MPa under water stress condition and cell turgor of male parent was significantly lower than that of the other 2 varieties. The volumetric elastic modulus of the cell wall is a property of rigid cell wall which represents the change in turgorof a cell caused by a given change of the relative volume. Value ofelastic modulus represented as male parent > female parent > F1 under the two water levels and enhanced by water stress significantly. Moreover, the differences of elastic modulusamong the three maize genotypes were significant under water stress condition. The half time of water exchange represented as male parent > female parent > F1 under the two water levels, and water stressprolongedhalf time; 50 μmol·L-1 HgCl2 also prolongedhalf time, but 5 mmol L-1 2-mercaptoethanol reversed the effect partly and the final half timewas even larger than the original value. The cell hydraulic conductivity represented as F1 > female parent > male parent under the two water levels, and water stress noticeably decreased cell hydraulic conductivity. The results indicated that F1 was better than the parents and showed heterosis in water uptake ability at cell level, and cell pressure probe worked well in the study of genotypic difference of cell water related parameters. The above results also provide scientific references for breeding and selecting new genotypes of maize with improved ability of water uptake by roots and with drought-resistance.

Key words: Pressure probe, Cell, Hydraulic conductivity, Water stress, Water uptake by roots, Heterosis


[1] Steudle E. The regulation of plant water at the cell, tissue and organ level: role of active processes and of compartmentation. In: Schulze ED. Flux control in biological systems: From enzymes to populations and ecosystems. San Diego: Academic Press, 1994. pp 237-299

[2] Azaizeh H, Steudle E. Effects of salinity on water transport of excised maize (Zea mays L.) roots. Plant Physiol, 1991, 97: 1136-1145

[3] Azaizeh H, Gunse B, Steudle E. Effects of NaCl and CaCl2 on water transport across cells of maize (Zea mays L.) seedlings. Plant Physiol, 1992, 99: 886-894

[4] Zhang W H, Tyerman S D. Inhibition of water channels by HgCl2 in intact wheat root cells. Plant Physiol, 1999, 120: 849-858

[5] Wan X C, Steudle E, Hartung W. Gating of water channels (aquaporins) in cortical cells of young corn roots by mechanical stimuli (pressure pulses): effects of ABA and of HgCl2. J Exp Bot, 2004, 55: 411-422

[6] Lee S H, Chung G C, Steudle E. Gating of aquaporins by low temperature in roots of chilling-sensitive cucumber and chilling-tolerant figleaf gourd. J Exp Bot, 2004, 56: 985-995

[7] Lee S H, Chung G C, Steudle E. Low temperature and mechanical stresses differently gateaquaporins of root cortical cells of chilling-sensitivecucumber and -resistant figleaf gourd. Plant Cell Environ, 2005, 28: 1191-1202

[8] Ye Q, Steudle E. Oxidative gating of water channels (aquaporins) in corn roots. Plant Cell Environ, 2006, 29: 459-470

[9] Lee S H, Singh A P, Chung1 GC, Ahn SJ, Noh EK, Steudle E. An exposure of roots of cucumber (Cucumis sativus) to low temperature severely reduces root pressure, hydraulic conductivity and active transport of nutrients. Physio Plant, 2004, 120: 413-420

[10] Mu Z-X(慕自新), ZHANG S-Q(张岁岐), LIANG A-H(梁爱华), LIANG Z-S(梁宗锁). Relationship between maize root hydraulic conductivity and drought resistance.Acta Agron Sin (作物学报), 2005, 31(2): 203-208(in Chinese with English abstract)

[11] Steudle E. Pressure probe techniques: basic principles and application to studies of water and solute relations at cell, tissue and organ level. In: Smith J A C, Griffiths H. Water Deficits: Plant Responses from Cell to Community. Oxford, UK: Bios Sclentific Publishers Ltd, 1993. pp 5-36

[12] Jones H G. Monitoring plant and soil water status: established and novel methods revisited and their relevance to studies of drought tolerance. J Exp Bot, 2007, 58: 119-130

[13] Taiz L. Plant cell expansion: regulation of cell wallmechanical properties. Annu Rev Plant Physiol, 1984, 35: 585-657

[14] Neumann P M. The role of cell wall adjustment in plant resistance to water deficits. Crop Sci, 1995, 35: 1258-1266



[15] Moorea J P,

[16] Neumann P M, Fan L. The spatially variable inhibition by water deficit of maize root growth correlates with altered profiles of proton flux and cell wall pH. Plant Physiol, 2004, 135: 2291-2300

[17] Fan L, Linker R, Gepstein S, Tanimoto E, Yamamoto R, Neumann P M. Progressive inhibition by water deficit of cell wall extensibility and growth along the elongation zone of maize roots is related to increased lignin metabolism and progressive stelar accumulation of wall phenolics. Plant Physiol, 2006, 140: 603-612

[18] Wan X C, Zwiazek J J. Mercuric chloride effects on root water transport in aspen seedlings. Plant Physiol, 2000, 121: 939-946

[19] Hukin D, Doering-Saad C, Thomas C R, Pritchard J. Sensitivity of cell hydraulic conductivity to mercury is coincident with symplasmic isolation and expression of plasmalemma aquaporin genes in growing maize roots. Planta, 2002, 215: 1047-1056

[20] Zhu M-J(朱美君), Chen J(陈珈), Wang X-C(王学臣). Identification, distributing and function of aquaporins on cytoplasm membranes of maize root cells.Chin Sci Bull (科学通报), 2000, 45(4): 407-411(in Chinese)

[21] Wu A-H(吴安慧), Zhang S-Q(张岁岐), Deng X-P(邓西平), Shan L(山仑). Expression of TIP1-1 in maize root systems under water deficit.Acta Agron Sin (作物学报), 2006, 32(9): 1413-1417(in Chinese with English abstract)
Wu A-H(吴安慧), Zhang S-Q(张岁岐), Deng X-P(邓西平), Shan L(山仑), Liu X-F(刘小芳). Expression of ZmPIP1 subgroup genes in maize roots under water shortage. J Plant Physiol Mol Biol (植物生理与分子生物学学报),2006, 32(5): 557-562(in Chinese with English abstract) Vicré-Gibouin M, Farrant J M, Driouich A.Adaptations of higher plant cell walls to water loss: Drought vs desiccation.Physiol Plant, 2008, 134: 237-245
[1] Zhang Xi, Wang Guang-En, Li Shao-Qi, Liu Yi, Li Jun-Lan, Qian Yu-Yuan. Transcriptome sequencing-based analysis on the formation mechanism of fiber micronaire differences between two sister lines derived from Gossypium hirsutum-G. barbadense hybrid [J]. Acta Agronomica Sinica, 2026, 52(5): 1442-1458.
[2] Qin Yi-Yan, Fu Yao, Su Chang, Li Na, Xu Jing-Ru, Cheng Xiao-Ran, Zhang Qi, Zhao Ming-Hui. Functional analysis of OsST41 regulating salt tolerance in rice seedlings [J]. Acta Agronomica Sinica, 2026, 52(3): 802-812.
[3] Zhang Qing, Yang Yu, Guo Qian, Yue Pei-Yao, Yin Cong-Cong, Niu Jing-Ping, Zhao Jin-Zhong, Du Wei-Jun, Yue Ai-Qin. Cloning and functional analysis of the soybean GmARA6a gene in response to salt stress [J]. Acta Agronomica Sinica, 2026, 52(2): 480-493.
[4] Yu Kai-Hang, Zhou Hong-Bin, Luo Liang-Zha, Wang Mei-Li, Jiang Rui-Mei, Dong-Chen Wen-Hua, Li Shi-Jin, Mao Xiao-Qiang, Chen Sheng-Wei. Cloning and expression analysis of the HvLRR-RLK-510 gene encoding a leucine-rich repeat receptor-like kinase in barley [J]. Acta Agronomica Sinica, 2026, 52(2): 421-432.
[5] LEI Song-Han, FAN Jun-Yang, CHE Yan-Yi, DAI Yong-Dong, ZHENG Yu-Meng, TIAN Wei-Jiang, SANG Xian-Chun, WANG Xiao-Wen. Identification of an adaxially-curled-leaf mutant acl3 and function analysis of the regulated gene in rice (Oryza sativa L.) [J]. Acta Agronomica Sinica, 2025, 51(6): 1467-1479.
[6] YUAN Xin, ZHAO Zhuo-Fan, ZHAO Rui-Qing, LIU Xiao-Wei, ZHENG Ming-Min, LIU Yu-Sheng, DONG Hao-Sheng, DENG Li-Juan, CAO Mo-Ju, HUANG Qiang. Genetic analysis and molecular identification of a small kernel mutant mn-like1 in maize [J]. Acta Agronomica Sinica, 2025, 51(6): 1569-1581.
[7] PAN Ju-Zhong, WEI Ping, ZHU De-Ping, SHAO Sheng-Xue, CHEN Shan-Shan, WEI Ya-Qian, GAO Wei-Wei. Cloning and functional analysis of OsERF104 transcription factor in rice [J]. Acta Agronomica Sinica, 2025, 51(4): 900-913.
[8] WANG Yu-Jiao, WANG Yong-Le, TIAN Chang-Jiu, YU Chun-Wang, LYU Jia-Bin, ZHU Jia-Bao. Cloning of the VQ4 gene and preliminary analysis of its role in salt tolerance in Coix lacryma-jobi L. [J]. Acta Agronomica Sinica, 2025, 51(12): 3198-3210.
[9] WANG Li-Ping, LI Pan, ZHAO Lian-Hao, FAN Zhi-Long, HU Fa-Long, FAN Hong, HE Wei, CHAI Qiang, YIN Wen. Response of senescence characteristics for maize leaves under different plastic mulching and using patterns in oasis irrigation areas of northwestern China [J]. Acta Agronomica Sinica, 2025, 51(1): 233-246.
[10] GAO Wei-Dong, HU Chen-Zhen, ZHANG Long, ZHANG Yan-Yan, ZHANG Pei-Pei, YANG De-Long, CHEN Tao. Cloning and functional analysis of ubiquitin-conjugating enzymes TaUBC16 gene in wheat [J]. Acta Agronomica Sinica, 2024, 50(8): 1971-1988.
[11] ZHU Zhong-Lin, WEN Yue, ZHOU Qi, WU Yan-Fei, DU Xue-Zhu, SHENG Feng. Mechanism of loding residence and drought tolerance of OsCNGC10 gene in rice [J]. Acta Agronomica Sinica, 2024, 50(5): 1351-1360.
[12] SONG Meng-Yuan, GUO Zhong-Xiao, SU Yu-Fei, DENG Kun-Peng, LAN Tian-Jiao, CHENG Yu-Xin, BAO Shu-Ying, WANG Gui-Fang, DOU Jin-Guang, JIANG Ze-Kai, WANG Ming-Hai, XU Ning. Transcriptome analysis of a stigma exsertion mutant in mungbean [J]. Acta Agronomica Sinica, 2024, 50(4): 957-968.
[13] ZHANG Bao-Hua, LIU Jia-Jing, TIAN Xiao, TIAN Xu-Zhao, DONG Kuo, WU Yu-Jie, XIAO Kai, LI Xiao-Juan. Cloning, expression, and functional analysis of wheat (Triticum aestivum L.) TaSPX1 gene in low nitrogen stress tolerance [J]. Acta Agronomica Sinica, 2024, 50(3): 576-589.
[14] HUANG Yu-Jie, ZHANG Xiao-Tian, CHEN Hui-Li, WANG Hong-Wei, DING Shuang-Cheng. Identification of ZmC2s gene family and functional analysis of ZmC2-15 under heat tolerance in maize [J]. Acta Agronomica Sinica, 2023, 49(9): 2331-2343.
[15] SONG Zhao-Jian, FENG Zi-Yi, QU Tian-Ge, LYU Pin-Cang, YANG Xiao-Lu, ZHAN Ming-Yue, ZHANG Xian-Hua, HE Yu-Chi, LIU Yu-Hua, CAI De-Tian. Indica-japonica attribute identification and heterosis utilization of diploid rice lines reverted from tetraploid rice [J]. Acta Agronomica Sinica, 2023, 49(8): 2039-2050.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd