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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (10): 1642-1648.doi: 10.3724/SP.J.1006.2010.01642

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

DNA Fingerprinting Analysis of 18 Cassava Varieties Using Sequence-Related Amplified Polymorphism Markers

JI  Lan1,2,WANG Wen-Quan3,ZHANG Zhen-Wen1,YE Jian-Qiu1,LI  Kai-Mian1*   

  1. 1 Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China; 2 Hainan University, Danzhou 571737, China; 3 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
  • Received:2010-03-23 Revised:2010-05-30 Online:2010-10-12 Published:2010-08-04
  • Contact: LI Kai-Mian,E-mail:likaimian@sohu.com

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Abstract: There widely exists a case of same name for more varieties or different names for one variety in cassava agriculture production, so it is difficult to identifycassava varieties on the basis of the phenotypic characters. In the paper, SRAP marker was applied to detect 18 cassava varieties, thirty-six primer combinations were selected with abundant polymorphism, a total of 320 bands were scored, 235 (73.4%) out of them were polymorphic, with an average of 8.9 bands and 6.5 polymorphic bands for each primer combination, 235 polymorphic bands then were used to develop a dendrogram with Unweighted Pair-Group Method Arithmetic Average (UPGMA), and 18 cassava varieties were divided into four major groups at the 0.734 similarity level; two primer combinations Me1-Em5 and Me24-Em10, were used to develop the DNA fingerprints for the 18 cassava varieties. The DNA fingerprints were then converted into binary codes, with 1 and 0 representing band presence and absence, respectively. In the DNA fingerprints, each of the 18 cassava varieties had its unique binary code, which distinguished varieties between each other easily, with 99.999% probability of confidence. The results demonstrated that it is feasible to distinguish varieties with DNA fingerprints established by SRAP.

Key words: Cassava, SRAP, DNA fingerprint

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