Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (10): 1649-1656.doi: 10.3724/SP.J.1006.2010.01649

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of Molecular Markers Associated with Seed Dormancy in Mini Core Collections of Chinese Wheat and Landraces

ZHANG Hai-Ping1,CHANG Cheng1*,YU Guang-Xia2,ZHANG Xiu-Ying2,YAN Chang-Sheng2,XIAO Shi-He2,SI Hong-Qi1,LU Jie1,MA Chuan-Xi1*   

  • Received:2010-03-19 Revised:2010-05-29 Online:2010-10-12 Published:2010-08-04
  • Contact: CHANG Cheng,E-mail:changtgw@126.com;MA Chuan-xin,E-mail:machuanxi@yahoo.cn E-mail:zhhp20@163.com

PDF

1974

Cited

5

Abstract: Seed dormancy evaluated by germination index (GI) is often regarded as a main and pivotal component of observed genetic variation for pre-harvest sprouting (PHS). Improving seed dormancy can decrease or avoid PHS damage to wheat (Triticum aestivum L.) before harvest, but it is a complicated trait controlled by multi-genes and influenced by many environmental factors. Because seed dormancy is difficult to be accurately evaluated under field condition, molecular markers will play an important role in dormancy evaluation. In this study, four SSR markers (Xbarc57, Xbarc294, Xbarc310, and Xbarc321) on the short arm of chromosome 3A and a gene-based marker (Vp1-b2) derived from Vp-1B on 3BL were used for genotyping 138 mini core collections of Chinese wheat and landraces. The results indicated that rich alleles occurred in the five markers, and most were significantly correlated with GI value. Based on general linear model, the five markers were significantly associated with seed dormancy. Markers Vp1-b2 and Xbarc294 had stronger effects on seed dormancy than other markers, accounting for 65.8% and 61.2% of seed dormancy variation, respectively. Marker combination could promote the percentage of phenotypic variation explained, of which the combination of five markers estimated 95.9% of the GI variation, followed by the combination of Vp1-b2 and Xbarc294 (89.1%), and the combination of Vp1-b2 and Xbarc321 had the smallest estimation of GI variation (79.4%). These results indicate that seed dormancy is mainly attributed to loci Vp1-b2 and Xbarc294 on 3AS and 3BL in the 138 genotypes, and the evaluation accuracy can be enhanced by jointly application of the total five markers in markers-assisted selection.

Key words: Triticum aestivum L., Seed dormancy, Molecular marker, Mini core collections of Chinese wheat

[1]Xiao S H, Zhang X Y, Yan C S, Hai L. Germplasm improvement for pre-harvest sprouting resistance in Chinese white-grained wheat: An overview of the current strategy. Euphytica, 2002, 126: 35-38
[2]Groos C, Gay G, Perretant M R, Gervais L, Bernard M, Dedryver F, Charmet G. Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a white×red grain bread wheat cross. Theor Appl Genet, 2002, 104: 39-47
[3]Watanabe N, Ikebata N. The effects of homologous group 3 chromosomes on grain color dependent seed dormancy and brittle rachis in tetraploid wheat. Euphytica, 2000, 115:215-220
[4]Torada A, Amano Y. Effect of seed coat color on seed dormancy in different environments. Euphytica, 2002, 126: 99-105
[5]Himi E, Mares D J, Yanagisawa A, Noda K. Effect of grain color gene (R) on grain dormancy and sensitivity of the embryo to abscisic acid (ABA) in wheat. J Exp Bot, 2002, 53: 1569-1574
[6]Roy J K, Prasad M, Varshney R K, Balyan H S, Blake T K, Dhaliwal H S, Singh H, Edwards K J, Gupta P K. Identification of a microsatellite on chromosomes 6B and a STS on 7D of bread wheat showing an association with pre-harvest sprouting tolerance. Theor Appl Genet, 1999, 99: 336-340
[7]Flintham J E. Different genetic components control coat imposed and embryo-imposed dormancy in wheat. Seed Sci Res, 2000, 10: 43-50
[8]Kato K, Nakamura W, Tabiki T, Miura H, Sawada S. Detection of loci controlling seed dormancy in group 4 chromosomes of wheat and comparative mapping with rice and barley genomes. Theor Appl Genet, 2001, 102: 980-985
[9]Kulwal P L, Singh R, Balyan H S, Gupta P K. Genetic basis of pre-harvest sprouting tolerance using single-locus and two-locus QTL analyses in bread wheat. Funct Integr Genomics, 2004, 4: 94-101
[10]Mares D, Mrva K, Cheong J, Williams K, Watson B, Storlie E, Sutherland M, Zou Y. A QTL located on chromosome 4A associated with dormancy in white- and red-grained wheats of diverse origin. Theor Appl Genet, 2005, 111: 1357-1364
[11]Mori M, Uchino N, Chono M, Kato K, Miura H. Mapping QTLs for grain dormancy on wheat chromosome 3A and group 4 chromosomes, and their combined effect. Theor Appl Genet, 2005, 110: 1315-1323
[12]Lohwasser U, Röder M S, Börner A. QTL mapping of the domestication traits pre-harvest sprouting and dormancy in wheat (Triticum aestivum L.). Euphytica, 2005, 143: 247-249
[13]Tan M K, Sharp P J, Lu M Q, Hows N. Genetics of grain dormancy in a white wheat. Aust J Agric Res, 2006, 57: 1157-1165
[14]Chen C X, Cai B, Bai G H. A major QTL controlling seed dormancy and pre-harvest sprouting resistance on chromosome 4A in a Chinese wheat landrace. Mol Breed, 2008, 21: 351-358
[15]Ogbonnaya F C, Imtiaz M, Ye G, Hearnden P R, Hernandez E, Eastwood R F, Van Ginkel M, Shorter S C, Winchester J M. Genetic and QTL analyses of seed dormancy and pre-harvest sprouting resistance in the wheat germplasm CN10955. Theor Appl Genet, 2008, 116: 891-902
[16]Munkvold J D, Tanaka J, Benscher D, Sorrells M E. Mapping quantitative trait loci for preharvest sprouting resistance in white wheat. Theor Appl Genet, 2009, 119: 1223-1235
[17]Mohan A, Kulwal P, Ravinder S, Vinay K, Reyazul R M, Kumar J, Prasad M, Balyan H S, Gupta P K. Genome-wide QTL analysis for pre-harvest sprouting tolerance in bread wheat. Euphytica, 2009, 168: 319-329
[18]Osa M, Kato K, Mori M, Shindo C, Torada A, Miura H. Mapping QTLs for seed dormancy and the Vp1 homologue on chromosome 3A in wheat. Theor Appl Genet, 2003, 106: 1491-1496
[19]Kottearachchi N S, Uchino N, Kato K, Miura H. Increased grain dormancy in white-grained wheat by introgression of pre-harvest sprouting tolerance QTLs. Euphytica, 2006, 152: 421-428
[20]Zhang X Q, Li C D, Tay A, Lance R, Mares D, Cheong J, Cakir M, Ma J H, Appels R. A new PCR-based marker on chromosome 4AL for resistance to pre-harvest sprouting in wheat (Triticum aestivum L.). Mol Breed, 2008, 22: 227-236
[21]Liu S B, Cai S B, Robert G, Chen C X, Bai G H. Quantitative trait loci for resistance to pre-harvest sprouting in US hard white winter wheat Rio Blanco. Theor Appl Genet, 2008, 117: 691-699
[22]Fofana B, Humphreys D G, Rasul G, Cloutier S, Brûlé-Babel A, Woods S, Lukow O M, Somers D J. Mapping quantitative trait loci controlling pre-harvest sprouting resistance in a red × white seeded spring wheat. Euphytica, 2009, 165: 509-521
[23]Rasul G, Humphreys D G, Brule-Babel A, McCartney C A, Knox R E, DePauw R M, Somers D J. Mapping QTLs for pre-harvest sprouting traits in the spring wheat cross ‘RL4452/AC Domain’. Euphytica, 2009, 168: 363-378
[24]Yang Y, Zhao X L, Xia L Q, Chen X M, Xia X C, Yu Z, He Z H, Röder M. Development and validation of a Viviparous-1 STS marker for pre-harvest sprouting tolerance in Chinese wheats. Theor Appl Genet, 2007, 115: 971-980
[25]Xia L Q, Ganal M W, Shewry P R, He Z H, Yang Y, Röder M S. Exploiting the diversity of Viviparous-1 gene associated with pre-harvest sprouting tolerance in European wheat varieties. Euphytica, 2008, 159: 411-417
[26]Chang C, Feng J M, Si H Q, Yin B, Zhang H P, Ma C X. Validating a novel allele of viviparous-1 (Vp-1Bf) associated with high seed dormancy of Chinese wheat landrace, Wanxianbaimaizi. Mol Breed, 2010, 25: 517-525
[27]Chang C, Zhang H P, Feng J M, Yin B, Si H Q, Ma C X. Identifying alleles of Viviparous-1B associated with pre-harvest sprouting in micro-core collections of Chinese wheat germplasm. Mol Breed, 2010, 25: 481-490
[28]Yang Y(杨燕), Zhao X-L(赵献林), Zhang Y(张勇), Chen X-M(陈新民), He Z-H(何中虎), Yu Z(于卓), Xia L-Q(夏兰琴). Evaluation and validation of four molecular markers associated with pre-harvest sprouting tolerance in Chinese wheats. Acta Agron Sin (作物学报), 2008, 34(1): 17-24 (in Chinese with English abstract)
[29]Zhang H-P(张海萍), Feng J-M(冯继明), Yin B(殷波), Chang C(常成), Si H-Q(司红起), Ma C-X(马传喜). Detection and validation of molecular marker associated with pre-harvest sprouting resistance in Chinese wheat. Mol Plant Breed (分子植物育种), 2009, 7(1): 45-50 (in Chinese with English abstract)
[30]Zhang H-P(张海萍), Chang C(常成), Zhang X-Y(张秀英), Yan C-S(闫长生), Xiao S-H(肖世和). Isolation and expression analysis of dehydrin gene involved in ABA-regulated seed embryo dormancy in wheat. Mol Plant Breed (分子植物育种), 2008, 6(6): 1175-1181 (in Chinese with English abstract)
[31]Zhang X-Y(张学勇), Pang B-S(庞斌双), You G-X(游光霞), Wang L-F(王兰芬), Jia J-Z(贾继增), Dong Y-C(董玉琛). Allelic variation and genetic diversity at Glu-1 loci in Chinese wheat (Triticum aestivum L.) germplasms. Sci Agric Sin (中国农业科学), 2002, 35(11): 1302-1310 (in Chinese with English abstract)
[32]Zhang H-P (张海萍), Chang C(常成), Feng J-M(冯继明), Yin B(殷波), Si H-Q(司红起), Ma C-X(马传喜). Detecting and identifying polymorphism of viviparous-B1 gene and its seed dormancy in mini-core collection of Chinese wheat varieties. J Agric Biotechnol (农业生物技术学报), 2009, l7(4): 690-694 (in Chinese with English abstract)
[33]Wang X-B(王晓波), Ma C-X(马传喜), Si H-Q(司红起), Qiao Y-Q(乔玉强), He X-F(何贤芳). Allelic variation of PPO genes in Chinese wheat micro-core collections. Sci Agric Sin (中国农业科学), 2009, 42(1): 28-35 (in Chinese with English abstract)
[34]Deng W-H(邓万洪), Yan B-J(晏本菊), Ren Z-L(任正隆). Application of molecular markers of Wx gene to screen partial Wx Wheat. J Triticeae Crops (麦类作物学报), 2007, 27(6): 982-998 (in Chinese with English abstract)
[35]Kang H W, Cho Y G, Yoon U H. A rapid DNA extraction method for RFLP and PCR analysis from a single dry seed. Plant Mol Biol Rep, 1998, 16: 1-9
[36]Huang Q-J(黄秦军), Ding M-M(丁明明), Zhang X-H(张香华), Su X-H(苏晓华). Association analysis between SSR molecular markers and wood properties of Populus nigra. Sci Silvae Sin (林业科学), 2007, 43 (2): 43-47 (in Chinese with English abstract)
[37]Hong Y-B(洪彦彬), Li S-X(李少雄), Liu H-Y(刘海燕), Zhou G-Y(周桂元), Chen X-P(陈小平), Wen S-J(温世杰), Liang X-Q(梁炫强). Correlation analysis of SSR markers and host resistance to Aspergillus flavus infection in peanut (Arachis hypogaea L.). Mol Plant Breed (分子植物育种), 2009, 7(2): 360-364 (in Chinese with English abstract)
[38]Elhan S E, Yu J M, Buckler E S. Applications of linkage disequilibrium and association mapping in maize. In: Kriz A L, Larkins B A, eds. Molecular Genetic Approaches to Maize Improvement. Berlin Heidelberg: Springer-Verlag, Biotechnology in Agriculture and Forestry, 2009. Vol. 63. pp 173-195
[39]Abecasis G R, Cookson W O C, Cardon L R. The power to detect linkage disequilibrium with quantitative traits in selected samples. Am J Hum Genet, 2001, 68: 1463-1474
[1] Zhai Sheng-Nan, Cao Xin-You, Li Hao-Sheng, Li Ji-Hu, Li Fa-Ji, Liu Jin-Dong, Xia Xian-Chun, Lyu Ying-Ying, Ma Rui-Feng, Wang Ying, Geng Hong-Wei, Liu Jian-Jun. Analysis of the genetic effects of allelic variation at the Pod-A1, Pod-D1, and Pod-2D loci on peroxidase activity in wheat grains [J]. Acta Agronomica Sinica, 2026, 52(6): 1593-1603.
[2] Peng Jia-Luo, Li Ying, Li Dan-Dan, Yang Jun-Ning, Guo Xue-Feng, Zhang Wen-Jiao, Yu Xiao-Xue, Zhou Ya-Rong, Wang Zhen-Yu, Wang Cai-Xiang, Ma Xiong-Feng, Su Jun-Ji. Identification of class I LBD family members in upland cotton and function and haplotype analyses of GhLBD6 in regulating flowering period [J]. Acta Agronomica Sinica, 2026, 52(6): 1682-1697.
[3] Zhang Xian-Feng, Guo Li-Jian, Li Kang-Chun, Kong Bin-Xue, Liu Yu-Fang, Che Zhuo, Yang De-Long. Identification of the ABHD6 gene family and development of functional markers for grain weight in wheat [J]. Acta Agronomica Sinica, 2026, 52(6): 1711-1727.
[4] Wang Chu-Rui, Li Kai-Xiang, Zhao Zhi, Xiao Lu, Tang Guo-Yong, Zhao Zhi-Gang, Xu Liang, Du De-Zhi, Liu Hai-Dong. Development and application of KASP markers for functional sites of the early flowering gene BnCRY2 in Brassica napus L. spring rapeseed [J]. Acta Agronomica Sinica, 2026, 52(3): 708-721.
[5] Wang Yue-Sheng, Ge Dong-Dong, Cheng Lan-Fei, Chen Chun-Huan, Wang Chang-You, Liu Xin-Lun, Li Ting-Dong, Deng Ping-Chuan, Ji Wan-Quan, Zhao Ji-Xin. Molecular cytogenetic and disease resistance characterization of the wheat- Psathyrostachys huashanica disomic substitution line 16DH25-7 [J]. Acta Agronomica Sinica, 2026, 52(2): 433-445.
[6] ZHAN Zong-Bing, JIN Qi-Feng, LIU Di, LYU Ying-Chun, GUO Ying, ZHANG Xue-Ting, HU Meng-Xia, WANG Shang, YANG Fang-Ping. Molecular characterization and evaluation of important traits of landrace wheat Laomangmai in Gansu province, China [J]. Acta Agronomica Sinica, 2025, 51(3): 609-620.
[7] XU Xiao-Wei, FENG Jing, WANG Feng-Tao, TONG Zhao-Yang, ZHANG Jian-Zhou, LI Chun-Ying, LIN Rui-Ming. QTL mapping of adult plant resistance to stripe rust in the Chinese wheat landrace Canlaomai [J]. Acta Agronomica Sinica, 2025, 51(11): 2933-2943.
[8] TIAN Han-Zhao, FENG Long-Ting, YING Kai, MENG Tian-Qi, WU Jun, LIU Yu-Xiu. Composition and evaluation of wheat glutenin subunits in exotic wheat germplasm [J]. Acta Agronomica Sinica, 2025, 51(10): 2663-2680.
[9] LIU Xin-Yuan, CHENG Yu-Kun, WANG Li-Li, ZHAN Shuai-Shuai, MA Meng-Yao, GUO Ling, GENG Hong-Wei. Allelic variation and distribution of peroxidase activity genes TaPod-A1, TaPod-A3, and TaPod-D1 of wheat in Xinjiang, China [J]. Acta Agronomica Sinica, 2025, 51(1): 68-78.
[10] FAN Zi-Pei, LI Long, SHI Yu-Gang, SUN Dai-Zhen, LI Chao-Nan, JING Rui-Lian. Cloning of TabHLH112-2B gene and development of its functional marker associated with the number of spikelet per spike in wheat [J]. Acta Agronomica Sinica, 2024, 50(2): 403-413.
[11] KE Hui-Feng, SU Hong-Mei, SUN Zheng-Wen, GU Qi-Shen, YANG Jun, WANG Guo-Ning, XU Dong-Yong, WANG Hong-Zhe, WU Li-Qiang, ZHANG Yan, ZHANG Gui-Yin, MA Zhi-Ying, WANG Xing-Fen. Identification for yield and fiber quality traits and evaluation of molecular markers in modern cotton varieties [J]. Acta Agronomica Sinica, 2024, 50(2): 280-293.
[12] CHEN Tian, LI Yu-Ying, RONG Er-Hua, WU Yu-Xiang. Character identification and floral organ transcriptome analysis on artificial allotetraploids of Gossypium hirsutum L. [J]. Acta Agronomica Sinica, 2024, 50(2): 325-339.
[13] TAN Dan, CHEN Jia-Ting, GAO Yu, ZHANG Xiao-Jun, LI Xin, YAN Gui-Yun, LI Rui, CHEN Fang, CHANG Li-Fang, ZHANG Shu-Wei, GUO Hui-Juan, CHANG Zhi-Jian, QIAO Lin-Yi. Discovery of auxin pathway genes involving spike type and association analysis between TaARF23-A and spikelet number in wheat [J]. Acta Agronomica Sinica, 2024, 50(2): 506-513.
[14] ZHAO Yang, LI Long, YANG Jin-Wen, JING Rui-Lian, SUN Dai-Zhen, WANG Jing-Yi. An E3 ubiquitin ligase gene TaSINA-3A is associated with plant height and 1000-grain weight in various environments in wheat [J]. Acta Agronomica Sinica, 2024, 50(10): 2654-2664.
[15] SONG Song-Quan, TANG Cui-Fang, LEI Hua-Ping, JIANG Xiao-Cheng, WANG Wei-Qing, CHENG Hong-Yan. Research progress of seed dormancy and germination regulation [J]. Acta Agronomica Sinica, 2024, 50(1): 1-15.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd