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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (10): 1649-1656.doi: 10.3724/SP.J.1006.2010.01649

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of Molecular Markers Associated with Seed Dormancy in Mini Core Collections of Chinese Wheat and Landraces

ZHANG Hai-Ping1,CHANG Cheng1*,YU Guang-Xia2,ZHANG Xiu-Ying2,YAN Chang-Sheng2,XIAO Shi-He2,SI Hong-Qi1,LU Jie1,MA Chuan-Xi1*   

  • Received:2010-03-19 Revised:2010-05-29 Online:2010-10-12 Published:2010-08-04
  • Contact: CHANG Cheng,E-mail:changtgw@126.com;MA Chuan-xin,E-mail:machuanxi@yahoo.cn E-mail:zhhp20@163.com

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Abstract: Seed dormancy evaluated by germination index (GI) is often regarded as a main and pivotal component of observed genetic variation for pre-harvest sprouting (PHS). Improving seed dormancy can decrease or avoid PHS damage to wheat (Triticum aestivum L.) before harvest, but it is a complicated trait controlled by multi-genes and influenced by many environmental factors. Because seed dormancy is difficult to be accurately evaluated under field condition, molecular markers will play an important role in dormancy evaluation. In this study, four SSR markers (Xbarc57, Xbarc294, Xbarc310, and Xbarc321) on the short arm of chromosome 3A and a gene-based marker (Vp1-b2) derived from Vp-1B on 3BL were used for genotyping 138 mini core collections of Chinese wheat and landraces. The results indicated that rich alleles occurred in the five markers, and most were significantly correlated with GI value. Based on general linear model, the five markers were significantly associated with seed dormancy. Markers Vp1-b2 and Xbarc294 had stronger effects on seed dormancy than other markers, accounting for 65.8% and 61.2% of seed dormancy variation, respectively. Marker combination could promote the percentage of phenotypic variation explained, of which the combination of five markers estimated 95.9% of the GI variation, followed by the combination of Vp1-b2 and Xbarc294 (89.1%), and the combination of Vp1-b2 and Xbarc321 had the smallest estimation of GI variation (79.4%). These results indicate that seed dormancy is mainly attributed to loci Vp1-b2 and Xbarc294 on 3AS and 3BL in the 138 genotypes, and the evaluation accuracy can be enhanced by jointly application of the total five markers in markers-assisted selection.

Key words: Triticum aestivum L., Seed dormancy, Molecular marker, Mini core collections of Chinese wheat

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