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Acta Agron Sin ›› 2010, Vol. 36 ›› Issue (3): 422-427.doi: 10.3724/SP.J.1006.2010.00422

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Mapping of a Bacterial Blight Resistance Gene Xa14 in Rice

BAO Si-Yuan1,2,TAN Ming-Pu3,LIN Xing-Hua1,*   

  1. 1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; 2 College of Basic Medical, Xianning University, Xianning 437100, China; 3 Life Science College, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2009-09-23 Revised:2009-10-02 Online:2010-03-12 Published:2009-12-21
  • Contact: LIN Xing-Hua，E-mail: xinghualin@mail.hzau.edu.cn

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Abstract:

Bacterial blight of rice, which is a vascular bundle disease caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases of rice worldwide, and leads to the yield of rice reduced greatly. The dominant gene Xa14 is highly resistant to Philippine race 5 of bacterial blight. The gene was located at the distal end on the long arm of chromosome 4 by Taura et al. Near-isogenic line in the background of IR24 namely IRBB14 carrying the geneXa14has been developed at International Rice Research Institute(IRRI). In order to construct the high-resolution likage maps for the Xa14 region on chromosome 4 for finally clone by positional cloning, tow F2 populations were used to estimate linkage based on marker genotype and reaction to disease inoculation with Xanthomonas oryzae pv. oryzae. Using 145 highly susceptible individuals from a total of 775 plants of F2 population of the cross between IRBB14 and IR24, the gene Xa14 was located in a 0.68 cM region on the nearby end of chromosome 4, which was flanked by the molecular markers, HZR970-8 and HZR988-1, with the distance of 0.34 cM between the flanked markers and Xa14, respectively, and completely cosegregated with the SSR markers, HZR645-4, HZR669-2, HZR669-5, and HZR669-7 in this population. Using 158 highly susceptible individuals from a total of 763 plants of F2 population of the cross between IRBB14 and ZZA, the gene Xa14 locus was mapped to the interval between HZR648-5 and RM280, and was 1.90 cM away from HZR648-5 which was the closest marker flanking the Xa14 locus. Combining recombination frequencies for the two populations together, the gene Xa14 was mapped to 3 BAC clones spanned approximately 300 kb in length between SSR markers HZR970-8 and HZR988-1.

Key words: Rice, Bacterial blight, Resistance gene, xa14, SSR, Genetic mapping


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