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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (01): 177-181.doi: 10.3724/SP.J.1006.2011.00177

• RESEARCH ACTIVITIES • Previous Articles     Next Articles

Effects of Exogenous Nitric Oxide Donor SNP on Lipid Peroxidation Caused by Selenium in Rice Seedlings

XIAO Qiang1,YANG Shu1,ZHENG Hai-Lei2,*   

  1. 1 Key Laboratory of Biological Resources Protection and Utilization of Hubei Province / Hubei Institutes for Nationalities, Enshi 445000, China; 2 School of Life Sciences, Xiamen University, Xiamen 361005, China
  • Received:2010-05-10 Revised:2010-09-19 Online:2011-01-12 Published:2010-11-16

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Abstract: Nitric oxide (NO) is a bioactive molecule that has been suggested to act as a signaling molecular in plants. It induces germination, affects plant growth and development, and promotes plant cell death. NO is also involved in plant response to heat, salinity, ultraviolet-B, and heavy metal stresses. It is known that some effects of NO may relate to the regulation of reactive oxygen species (ROS) metabolism by means of affecting activities of catalase (CAT) with hemachrome iron, and/or guaiacol peroxidase (GPX) with none-hemachrome iron. However, whether NO regulates lipid peroxidation in rice seedlings induced by selenium is not yet understood. In this article, we reported some regulative  effects of exogenous nitric oxide donor SNP on oxidative stresses induced by selenium in rice seedlings. The contents of chlorophyll, H2O2, TBARS and the activities of GPX, superoxide dismutase (SOD), CAT and ascorbate peroxidase (APX) in rice seedlings treated with a varying concentrations of seleniumand 1 μmol L-1SNP were investigated. The results showed that the content of chlorophyll increased by treatment with SNP in 0.2 μmol L-1Na2SeO3 group. SNP alleviated significantlythe lipid peroxidation in rice seedlings via promoting GPX, APX and CAT activities in rice leaf. In 20 μmol L-1Na2SeO3 treated rice seedlings, SNPalleviated significantlyTBARScontent and the increase of H2O2 content that resulted from high selenium stress via promoting GPX, especially APX and CAT activities. Taken together, our results suggested that NO regulates lipid peroxidation caused by selenium in rice seedlings.

Key words: Oryza sativa, Selenium, Sodium nitroprusside, Lipid peroxidation

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