Welcome to Acta Agronomica Sinica,

Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (03): 423-430.doi: 10.3724/SP.J.1006.2018.00423


Effects of Foliage Spray of Se on Absorption Characteristics of Se and Quality of Purple Sweet Potato

Song HOU(), Xia TIAN, Qing LIU*()   

  1. College of Resources and Environment, Qingdao Agricultural University, Qingdao 266109, Shandong, China
  • Received:2017-07-27 Accepted:2018-11-21 Online:2018-03-12 Published:2017-12-18
  • Contact: Qing LIU E-mail:hhousong@163.com;qy7271@163.com
  • Supported by:
    This study was supported by China Agriculture Research System (CARS-10-B10), the Key Project of the Shandong Research and Development Program (2016GNC110020), and Special Projects for Public Welfare Research (201303106).


In order to clarify the absorption characteristics of selenium (Se), root yield, Se utilization, content of amino acids and mineral elements in purple sweet potato, a field experiment of foliage spraying with three treatments of 0, 30, and 60 g ha-1 pure Se application were carried out in 2015 and 2016 respectively. The root yield of purple sweet potato received no significantly effect from Se foliage spray, the Se content, organic Se content and the Se accumulation in root were improved, with a root Se content of 311.3 µg kg-1 (DW), 8.54 times as high as that of the control under 60 g ha-1 Se application. Se content and accumulation amount in root increased and the utilization rate of Se decreased significantly with the increase of Se application, while the content of organic Se in root received no significantly effect from Se foliage spray. With increasing Se application, the content of crude protein in root of purple sweet potato was significantly increased, which was 11.67% and 29.71% higher than that of CK under Se treatment of 30 g ha-1 and 60 g ha-1 respectively. Under 60 g ha-1 Se application treatment, seven essential amino acids for human body contained in roots increased significantly except Threonine, and the other six nonessential amino acids in roots also increased significantly such as Asparagine, Serine, Glutamic acid, but the content of Cysteine decreased. Se application treatments increased the contents of K, Na, Mg, Fe, Cu and decreased the contents of Ca, Mn in roots of purple sweet potato, but had no effect on content of Zn. The results of this study are of significance guiding production of Se-enriched purple sweet potato.

Key words: purple sweet potato, selenium, absorption and accumulation, protein, amino acid, mineral elements, foliar spraying

Fig. 1

Yield of root tuber of purple sweet potato under different selenium treatments In each growing season, bars superscripted by the same letters are not significantly different at P < 0.05."

Table 1

Concentration of total selenium in organs of purple sweet potato under different selenium treatments (µg kg-1 DW)"

各器官含硒量 Se content in organs
块根 Root tuber 茎 Stem 叶 Leaf 叶柄 Petiole
2015 CK 39.9±8.2 c 54.2±7.4 c 156.3±12.4 c 63.7±9.7 c
Se1 209.7±25.3 b 289.1±28.7 b 503.9±47.6 b 277.0±32.1 b
Se2 301.6±33.2 a 680.1±45.3 a 1333.5±105.7 a 589.3±62.3 a
2016 CK 33.5±6.1 c 50.3±6.5 c 134.8±18.3 c 55.4±6.8 c
Se1 189.1±23.4 b 266.9±30.2 b 487.6±55.9 b 283.6±27.2 b
Se2 311.3±38.7 a 649.6±51.9 a 1299.0±111.2 a 567.8±58.4 a

Table 2

Concentration of organic selenium in root tuber and the ratios to total selenium under different selenium treatments"

Total selenium
(µg kg-1 DW)
Organic selenium
(µg kg-1 DW)
Ratios of organic selenium to total selenium (%)
2015 CK 39.9±8.2 c 30.6±7.6 c 76.81 a
Se1 209.7±25.3 b 154.1±22.3 b 73.43 a
Se2 301.6±33.2 a 229.2±30.8 a 75.96 a
2016 CK 33.5±6.1 c 25.9±6.4 c 77.33 a
Se1 189.1±23.4 b 140.5±22.9 b 74.25 a
Se2 311.3±38.7 a 244.6±38.1 a 78.56 a

Table 3

Selenium accumulation and utilization rate under different selenium treatments"

Concentration of
selenium in root tuber
(µg kg-1 DW)
Yield of root tuber
(t hm-2 FW)
Moisture content
in root tuber (%)
Selenium accumulation in root tuber (mg hm-2)
Utilization ratio of selenium in root tuber (%)
2015 CK 39.9±8.2 c 31.6±3.66 a 71.24 362.6
Se1 209.7±25.3 b 30.7±4.24 a 70.83 1877.9 5.05
Se2 301.6±33.2 a 29.6±3.58 a 70.36 2646.1 3.81
2016 CK 36.5±6.1 c 30.7±4.01 a 71.05 324.4
Se1 189.1±23.4 b 29.6±3.23 a 70.77 1636.1 4.37
Se2 311.3±38.7 a 28.9±3.79 a 71.39 2573.9 3.75

Fig. 2

Concentration of crude protein in organs of purple sweet potato under different selenium treatments In each growing season, bars superscripted by different letters are significantly different at P < 0.05."

Fig. 3

Concentration of eight essential amino acids of humanbody in root tuber of purple sweet potato under different selenium treatments Bars superscripted by different letters are significantly different at P < 0.05."

Fig. 4

Concentration of ten nonessential amino acids of humanbody in root tuber of purple sweet potato under different selenium treatments Bars superscripted by different letters are significantly different at P < 0.05."

Table 4

Concentration of mineral elements in root tuber under different selenium treatments (mg kg-1)"

处理 K Na Ca Mg Fe Mn Zn Cu
CK 7642.3 b 3762.4 b 2970.8 a 1202.1 b 163.47 b 19.09 a 35.03 a 8.56 b
Se1 8184.4 b 3603.7 b 2693.8 b 1147.4 b 180.71 b 17.33 a 36.45 a 9.39 b
Se2 9475.7 a 4014.8 a 2410.0 b 1455.1 a 255.44 a 11.56 b 37.04 a 12.06 a
[1] 李继云, 任尚学, 陈代中. 陕西省环境中的硒与大骨节病关系的研究. 环境科学学报, 1982, 2: 91-101
Li J Y, Ren S X, Chen D Z.A study of kaschi-beck disease associated with environmental selenium in Shaanxi area.Acta Sci Circumst, 1982, 2: 91-101 (in Chinese with English abstract)
[2] 陈铭, 谭见安, 王五一. 环境硒与健康关系研究中的土壤化学与植物营养学. 土壤学进展, 1994, 22(4): 1-10
Chen M, Tan J A, Wang W Y.Soil chemistry and plant nutrition of environmental selenium and healthy relations.Prog Soil Sci, 1994, 22(4): 1-10 (in Chinese with English abstract)
[3] 彭安, 王子健, Whnager P D, Combs G F, Yeh J Y.硒的环境生物无机化学. 北京: 中国环境科学出版社, 1995. pp 11-12
Peng A, Wang Z J, Whanger P D, Combs G F, Yeh J Y.Environmental Bio-inorganic Chemistry of Selenium. Beijing: China Environmental Science Press, 1995. pp 11-12 (in Chinese)
[4] 刘庆, 田侠, 史衍玺. 施硒对小麦籽粒硒富集、转化及蛋白质与矿质元素含量的影响. 作物学报, 2016, 42: 778-783
Liu Q, Tian X, Shi Y X.Effects of Se application on Se accumulation and transformation and content of gross protein and mineral elements in wheat grain.Acta Agron Sin, 2016, 42: 778-783 (in Chinese with English abstract)
[5] Chilimba A D C, Young S D, Black C R, Meacham M C, Lammel J, Broadley M R. Agronomic biofortification of maize with selenium (Se) in Malawi.Field Crops Res, 2012, 125: 118-128
[6] 李韬, 兰国防. 植物硒代谢机理及其以小麦为载体进行补硒的策略. 麦类作物学报, 2012, 32: 173-177
Li T, Lan G F.Metabolism of selenium in plants and the strategies for selenium biofortification using wheat.J Triticeae Crops, 2012, 32: 173-177 (in Chinese with English abstract)
[7] Brown T A, Shrift A.Selenium: toxicity and tolerance in higher plants.Biol Rev, 1982, 57: 59-84
[8] 彭琴, 李哲, 梁东丽, 王梦柯, 郭璐. 不同作物对外源硒动态吸收/转运的差异及其机制. 环境科学, 2017, 38: 1667-1674
Peng Q, Li Z, Liang D L, Wang M K, Guo L.Dynamic differences of uptake and translocation of exogenous selenium by different crops and its mechanism. Environ Sci, 2017, 38: 1667-1674 (in Chinese with English abstract)
[9] Keskinen R, Turakainen M, Hartikainen H.Plant availability of soil selenate additions and selenium distribution within wheat and ryegrass.Plant & Soil, 2010, 333: 301-313
[10] 周鑫斌, 施卫明, 杨林章. 富硒与非富硒水稻品种对硒的吸收分配的差异及机理. 土壤, 2007, 39: 731-736
Zhou X B, Shi W M, Yang L Z.Genotypical differences and characteristics of Se uptake and accumulation in rice. Soils, 2007, 39: 731-736 (in Chinese with English abstract)
[11] 陈思杨, 江荣风, 李花粉. 苗期小麦和水稻对硒酸盐/亚硒酸盐的吸收及转运机制. 环境科学, 2011, 32: 284-289
Chen S Y, Jiang R F, Li H F.Uptake and translocation of selenate or selenite by wheat and rice seedlings.Environ Sci, 2011, 32: 284-289 (in Chinese with English abstract)
[12] 殷金岩, 耿增超, 李致颖, 李慧娟. 硒肥对马铃薯硒素吸收、转化及产量、品质的影响. 生态学报, 2015, 35: 823-829
Yin J Y, Geng Z C, Li Z Y, Li H J.Effects of three fertilizers on uptake, transformation, yield and quality of potatoes.Acta Ecol Sin, 2015, 35: 823-829 (in Chinese with English abstract)
[13] Li H F, McGrath S P, Zhao F J. Selenium uptake, translocation and speciation in wheat supplied with selenate or selenite.New Phytologist, 2008, 178: 92-102
[14] Yao X, Chu J, He X, Ba C.Protective role of selenium in wheat seedlings subjected to enhanced UV-B radiation.Russian J Plant Physiol, 2011, 58: 283-289
[15] Hasanuzzaman M, Fujita M.Selenium pretreatment up regulates the antioxidant defense and methylglyoxal detoxification system and confers enhanced tolerance to drought stress in rapeseed seedlings.Biol Trace Element Res, 2011, 143: 1758-1776
[16] 李彦, 史衍玺, 张英鹏, 董晓霞, 孙明. 盐胁迫条件下硒对小白菜抗氧化活性及膜脂过氧化作用的影响. 植物营养与肥料学报, 2008, 14: 749-753
Li Y, Shi Y X, Zhang Y P, Dong X X, Sun M.Effects of selenium on the antioxidant capacity and membrane lipid peroxidation of pakchoi cabbage under salt stress.Plant Nutr Fert Sci, 2008, 14: 749-753 (in Chinese with English abstract)
[17] Feng R, Wei C, Tu S.The roles of selenium in protecting plants against abiotic stresses.Environ Exp Bot, 2013, 87: 58-68
[18] 郭文慧, 刘庆, 史衍玺. 施硒对紫甘薯硒素累积及产量和品质的影响研究. 中国粮油学报, 2016, 31(9): 31-36
Guo W H, Liu Q, Shi Y X.Effects of applying selenium on the accumulation of selenium, yield, and quality of purple sweetpotato.J Chin Cereals Oils Assoc, 2016, 31(9): 31-36 (in Chinese with English abstract)
[19] 中华人民共和国卫生部. GB/T5009.93-2010. 食品安全国家标准: 食品中硒的测定. 2010
Ministry of Health of the People’s Republic of China. GB/T5009. 93-2010. National Food Safety Standard: Determination of Selenium in Foods, 2010 (in Chinese)
[20] Sun M, Liu G J, Wu Q H.Speciation of organic and inorganic selenium in selenium-enriched rice by graphite furnace atomic absorption spectrometry after cloud point extraction. Food Chem, 2013, 141: 66-71
[21] 鲍士旦. 土壤农化分析(第3版). 北京: 中国农业出版社, 2005.
Bao S D.Soil and Agricultural Chemistry Analysis (3rd edn). Beijing: China Agriculture Press, 2005 (in Chinese)
[22] 穆婷婷, 杜慧玲, 张福耀, 景小兰, 郭琦, 李志华, 刘璋, 田岗. 外源硒对谷子生理特性、硒含量及其产量和品质的影响. 中国农业科学, 2017, 50: 51-63
Mu T T, Du H L, Zhang F Y, Jing X L, Guo Q, Li Z H, Liu Z, Tian G.Effects of exogenous selenium on the physiological activity, grain selenium content, yield and quality of foxtail millet.Sci Agric Sin, 2017, 50: 51-63 (in Chinese with English abstract)
[23] 张新军, 杨才, 曾昭海, 田长叶, 胡跃高. 叶面喷施硒肥对裸燕麦产量和品质的影响. 麦类作物学报, 2015, 35: 408-412
Zhang X J, Yang C, Zeng Z H, Tian C Y, Hu Y G.Effect of Se foliar fertilizer rate on yield and quality of naked oat (Avena nuda). J Triticeae Crops, 2015, 35: 408-412 (in Chinese with English abstract)
[24] 李春霞, 曹慧. 植物硒的营养特点及吸收转化机理研究进展. 农业科学研究, 2006, 27(4): 72-76
Li C X, Cao H.The research overview of the nutrition characteristics, absorption and transformation of the plant selenium.J Agric Sci, 2006, 27(4): 72-76 (in Chinese with English abstract)
[25] Sajedi N, Ardakani M, Madani H, Naderi A, Miransari M.The effects of selenium and other micronutrients on the antioxidant activities and yield of corn (Zea mays L.) under drought stress. Physiol Mol Biol Plants, 2011, 17: 215-222
[26] 李永华, 王五一. 硒的土壤环境化学研究进展. 土壤通报, 2002, 33: 230-233
Li Y H, Wang W Y.Progress on the study of soil environmental chemistry of selenium.Chin J Soil Sci, 2002, 33: 230-233 (in Chinese with English abstract)
[27] 胡秋辉, 朱建春, 潘根兴. 硒的土壤生态环境、生物地球化学与食物链的研究现状. 农村生态环境, 2000, 16(4): 54-57
Hu Q H, Zhu J C, Pan G X.Biological geochemistry and selenium in food chain.Rural Eco-environ, 2000, 16(4): 54-57 (in Chinese with English abstract)
[28] 杨玉玲. 硒对大豆籽粒中硒代氨基酸和辅酶Q10含量的影响. 东北农业大学博士学位论文, 黑龙江哈尔滨, 2014
Yang Y L.Effect of Selenium on the Content of Seleno Amino Acids and Coenzyme Q10 in Soybean Seeds. PhD Dissertation of Northeast Agricultural University, Harbin,China, 2014 (in Chinese with English abstract)
[29] Curtin D, Hanson R, Lindley T N, Butler R C.Selenium concentration in wheat (Triticum aestivum) grain as influenced by method, rate, and timing of sodium selenate application. N Z J Exp Agric, 2006, 34: 329-339
[1] WANG Wang-Nian, GE Jun-Zhu, YANG Hai-Chang, YIN Fa-Ting, HUANG Tai-Li, KUAI Jie, WANG Jing, WANG Bo, ZHOU Guang-Sheng, FU Ting-Dong. Adaptation of feed crops to saline-alkali soil stress and effect of improving saline-alkali soil [J]. Acta Agronomica Sinica, 2022, 48(6): 1451-1462.
[2] YANG Jian-Chang, LI Chao-Qing, JIANG Yi. Contents and compositions of amino acids in rice grains and their regulation: a review [J]. Acta Agronomica Sinica, 2022, 48(5): 1037-1050.
[3] ZHU Zheng, WANG Tian-Xing-Zi, CHEN Yue, LIU Yu-Qing, YAN Gao-Wei, XU Shan, MA Jin-Jiao, DOU Shi-Juan, LI Li-Yun, LIU Guo-Zhen. Rice transcription factor WRKY68 plays a positive role in Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae [J]. Acta Agronomica Sinica, 2022, 48(5): 1129-1140.
[4] JIN Min-Shan, QU Rui-Fang, LI Hong-Ying, HAN Yan-Qing, MA Fang-Fang, HAN Yuan-Huai, XING Guo-Fang. Identification of sugar transporter gene family SiSTPs in foxtail millet and its participation in stress response [J]. Acta Agronomica Sinica, 2022, 48(4): 825-839.
[5] XU Jing, GAO Jing-Yang, LI Cheng-Cheng, SONG Yun-Xia, DONG Chao-Pei, WANG Zhao, LI Yun-Meng, LUAN Yi-Fan, CHEN Jia-Fa, ZHOU Zi-Jian, WU Jian-Yu. Overexpression of ZmCIPKHT enhances heat tolerance in plant [J]. Acta Agronomica Sinica, 2022, 48(4): 851-859.
[6] YANG Zong-Tao, LIU Shu-Xian, CHENG Guang-Yuan, ZHANG Hai, ZHOU Ying-Shuan, SHANG He-Yang, HUANG Guo-Qiang, XU Jing-Sheng. Sugarcane ubiquitin-like protein UBL5 responses to SCMV infection and interacts with SCMV-6K2 [J]. Acta Agronomica Sinica, 2022, 48(2): 332-341.
[7] WANG Na, BAI Jian-Fang, MA You-Zhi, GUO Hao-Yu, WANG Yong-Bo, CHEN Zhao-Bo, ZHAO Chang-Ping, ZHANG Ling-Ping. Cloning and expression analysis of lncRNA27195 and its target gene TaRTS in wheat (Triticum aestivum L.) [J]. Acta Agronomica Sinica, 2021, 47(8): 1417-1426.
[8] ZHANG Hai, CHENG Guang-Yuan, YANG Zong-Tao, LIU Shu-Xian, SHANG He-Yang, HUANG Guo-Qiang, XU Jing-Sheng. Sugarcane PsbR subunit response to SCMV infection and its interaction with SCMV-6K2 [J]. Acta Agronomica Sinica, 2021, 47(8): 1522-1530.
[9] GAO Lu, XU Wen-Liang. GhP4H2 encoding a prolyl-4-hydroxylase is involved in regulating cotton fiber development [J]. Acta Agronomica Sinica, 2021, 47(7): 1239-1247.
[10] LI Jie, FU Hui, YAO Xiao-Hua, WU Kun-Lun. Differentially expressed protein analysis of different drought tolerance hulless barley leaves [J]. Acta Agronomica Sinica, 2021, 47(7): 1248-1258.
[11] SU Ya-Chun, LI Cong-Na, SU Wei-Hua, YOU Chui-Huai, CEN Guang-Li, ZHANG Chang, REN Yong-Juan, QUE You-Xiong. Identification of thaumatin-like protein family in Saccharum spontaneum and functional analysis of its homologous gene in sugarcane cultivar [J]. Acta Agronomica Sinica, 2021, 47(7): 1275-1296.
[12] ZHANG Zhi-Xing, CHEN Hua, MIN Xiu-Mei, XU Hai-Long, SONG Guo, LIN Wen-Xiong. Preliminary study of the peptide aptamer R18 promotes grain filling of rice inferior spikelets [J]. Acta Agronomica Sinica, 2021, 47(7): 1332-1341.
[13] MA Yan-Bin, WANG Xia, LI Huan-Li, WANG Pin, ZHANG Jian-Cheng, WEN Jin, WANG Xin-Sheng, SONG Mei-Fang, WU Xia, YANG Jian-Ping. Transformation and molecular identification of maize phytochrome A1 gene (ZmPHYA1) in cotton [J]. Acta Agronomica Sinica, 2021, 47(6): 1197-1202.
[14] TANG Jing-Quan, WANG Nan, GAO Jie, LIU Ting-Ting, WEN Jing, YI Bin, TU Jin-Xing, FU Ting-Dong, SHEN Jin-Xiong. Bioinformatics analysis of SnRK gene family and its relation with seed oil content of Brassica napus L. [J]. Acta Agronomica Sinica, 2021, 47(3): 416-426.
[15] MENG Yu-Yu, WEI Chun-Ru, FAN Run-Qiao, YU Xiu-Mei, WANG Xiao-Dong, ZHAO Wei-Quan, WEI Xin-Yan, KANG Zhen-Sheng, LIU Da-Qun. TaPP2-A13 gene shows induced expression pattern in wheat responses to stresses and interacts with adaptor protein SKP1 from SCF complex [J]. Acta Agronomica Sinica, 2021, 47(2): 224-236.
Full text



No Suggested Reading articles found!