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Acta Agronomica Sinica ›› 2018, Vol. 44 ›› Issue (03): 431-441.doi: 10.3724/SP.J.1006.2018.00431

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY • Previous Articles     Next Articles

Influences of Rape Intercropping with Chinese Milk Vetch and Straw Mulching on Productive Benefits in Dryland of Southwest China

Quan ZHOU1,2(), Long-Chang WANG1,*(), Shu-Min MA1, Xiao-Duan ZHANG1, Yi XING1, Sai ZHANG1   

  1. 1 College of Agronomy and Biotechnology, Southwest University / Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education / Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400716, China
    2 Research Center on Ecological Sciences, Jiangxi Agricultural University / Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Nanchang 330045, Jiangxi, China
  • Received:2017-05-15 Accepted:2017-11-21 Online:2018-03-12 Published:2017-12-11
  • Contact: Long-Chang WANG E-mail:zhouquanyilang@163.com;wanglc2003@163.com
  • Supported by:
    This study was supported by the Special Fund for Agro-scientific Research in the Public Interest (201503127) and the National Natural Science Foundation of China (31271673).

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Abstract:

In dryland of the southwest of China, the eco-environment of farmland is extremely fragile, and the application of green manure is not enough. The seasonal drought is a key factor limiting the rape production. In order to improve rape yield and explore the application method of green manure, we compared the effects of intercropping and straw mulching on photosynthetic characteristics, agronomic traits, biomass, root morphology, rapeseed quality and yield. Intercropping Chinese milk vetch and straw mulching substantially improved the agronomic traits (diameter of root crown, leaf number, effective branch number and number of pods per plant), the root morphology (total root length, total root volume, total root surfarea and average root diameter), and the biomass of root, stem and leaf at stem elongation, flowering and podding stages of rape. At the same time, it also increased the photosynthetic rate at seeding stage. Water use efficiency in intercropping treatment and straw mulching treatment was increased by 31.12% and 39.89% as compared with rape monoculture. In addition, treatments of intercropping, straw mulching and intercropping with straw mulching significantly increased rape yield by 10.30%, 13.20%, and 40.16% as compared with rape monoculture. Our findings revealed that intercropping Chinese milk vetch and straw mulching are helpful to improve the agronomic trait, biomass and root morphology, resulting in yield increase in rape.

Key words: green manure, intercropping, root interaction, root morphology, rape yield

Fig. 1

Effects of intercropping Chinese milk vetch and straw mulching on agronomic traits of rape (2014-2015) F: full root separation with plastic film; P: partial root separation with nylon nets; N: no root separation; T: no straw mulching; S: straw mulching. Bars superscribed by different letters are significantly different at P<0.05."

Fig. 2

Effects of intercropping Chinese milk vetch and straw mulching on root morphology of rape (2014-2015) F: full root separation with plastic film; P: partial root separation with nylon nets; N: no root separation; T: no straw mulching; S: straw mulching. Bars superscribed by different letters are significantly different at P<0.05."

Table 1

Effects of intercropping Chinese milk vetch and straw mulching on photosynthetic characteristics at seedling stage (2015-2016)"

处理
Treatment		 净光合速率
Net photosynthetic rate (Pn)
(μmol m-2 s-1)		 气孔导度
Stomatal
conductance (Gs)
(mol m-2 s-1)		 胞间CO2浓度
Intercellular CO2 concentration (Ci)
(μmol mol-1)		 蒸腾速率
Transpiration rate (Tr)
(mmol m-2 s-1)		 水分利用效率
Water use efficiency (WUE)
(mmol mol-1)
油菜单作+无秸秆覆盖R 6.77±0.77 b 0.12±0.06 b 300.01±70.23 a 0.58±0.24 b 8.90±1.19 a
紫云英油菜间作+无秸秆覆盖AR 7.60±0.51 ab 0.15±0.06 ab 326.96±29.01 a 0.69±0.20 ab 11.67±3.35 a
油菜单作+秸秆覆盖SR 8.12±1.33 ab 0.26±0.04 a 376.22±18.91 a 1.06±0.17 a 7.89±2.54 a
紫云英油菜间作+秸秆覆盖SAR 8.74±0.51 a 0.15±0.05 ab 332.07±32.96 a 0.73±0.19 ab 12.45±2.78 a

Fig. 3

Effects of intercropping Chinese milk vetch and straw mulching on biomass of rape (2015-2016) R: rape monoculture + no straw mulching; AR: rape intercropping with Chinese milk vetch + no straw mulching; SR: rape monoculture + straw mulching; SAR: rape intercropping with Chinese milk vetch + straw mulching. Bars superscribed by different letters are significantly different at P<0.05."

Fig. 4

Effects of intercropping Chinese milk vetch and straw mulching on oil content, protein and glucosinolates content of rape (2015-2016) R: rape monoculture + no straw mulching; AR: rape intercropping with Chinese milk vetch + no straw mulching; SR: rape monoculture + straw mulching; SAR: rape intercropping with Chinese milk vetch + straw mulching. Bars superscripted by different letters are significantly different at P<0.05."

Table 2

Effects of intercropping Chinese milk vetch and straw mulching on fatty acids of rape (2015-2016) (%)"

脂肪酸
Fatty acid		 油菜单作+
无秸秆覆盖R		 紫云英油菜间作+
无秸秆覆盖AR		 油菜单作+
秸秆覆盖SR		 紫云英油菜间作+
秸秆覆盖SAR
低芥酸Low erucic acid 0.29±0.28 a 0.30±0.23 a 0.12±0.09 a 0.40±0.14 a
高芥酸High erucic acid 9.14±0.16 a 6.17±4.50 a 5.13±3.73 a 8.15±4.08 a
高油酸High oleic acid 62.73±0.94 a 62.02±0.14 a 61.47±1.23 a 61.51±0.66 a
花生烯酸Arachidonic acid 3.04±0.60 a 3.22±0.66 a 3.47±1.37 a 3.62±0.47 a
芥酸Erucic acid 5.18±0.52 a 4.33±1.45 a 3.14±1.84 a 5.49±2.11 a
亚麻酸Linolenic acid 9.05±0.32 a 8.56±0.28 ab 8.49±0.16 b 8.88±0.08 ab
亚油酸Linoleic acid 21.85±0.67 ab 21.29±0.62 ab 20.76±0.25 b 22.32±1.06 a
硬脂酸Stearic acid 0.57±0.11 a 0.40±0.13 b 0.36±0.11 b 0.38±0.16 b
油酸Oleic acid 55.54±1.20 a 58.23±2.83 a 59.60±2.06 a 55.38±1.84 a
棕榈酸Palmitic acid 4.66±0.09 b 4.78±0.03 ab 4.86±0.24 ab 4.90±0.08 a

Fig. 5

Effects of intercropping Chinese milk vetch and straw mulching on yield per plant of rape (2014-2015) F: full root separation with plastic film; P: partial root separation with nylon nets; N: no root separation; T: no straw mulching; S: straw mulching. Bars superscripted by different letters are significantly different at P<0.05."

Fig. 6

Effects of intercropping Chinese milk vetch and straw mulching on population yield of rape (2015-2016) R: rape monoculture + no straw mulching; AR: rape intercropping with Chinese milk vetch + no straw mulching; SR: rape monoculture + straw mulching; SAR: rape intercropping with Chinese milk vetch + straw mulching. Bars superscribed by different letters are significantly different at P<0.05."

Table 3

Correlation coefficients of agronomic traits and root morphological parameters with yield of rape"

农艺性状
Agronomic trait		 单株产量
Yield per plant		 根系形态
Root morphology		 单株产量
Yield per plant
株高Plant height 0.578* 总根长Total root length -0.009
根颈粗Diameter of root crown 0.827** 总根表面积Total root surfarea 0.493*
叶片数Leaf number 0.770** 平均根系直径Average root diameter 0.426
无柄叶数Sessile leaf number 0.774** 总根体积Total root volume 0.659**
一级有效分枝数Primary effective branch number 0.679** 根尖数Tips 0.089
二级有效分枝数Secondary effective branch number 0.723** 分枝数Forks 0.046
单株角果数Number of pods per plant 0.880** 交叉数Crossings -0.066

Table 4

Grey judgement analysis of agronomic traits and root morphology"

处理
Treatment		 关联系数 Correlation coefficient (ξ) 灰色综合
评判值
G
株高
PH		 根颈粗
DRB		 叶片数
LN		 无柄叶数
SLN		 一级有效分枝数
PEBN		 二级有效分枝数
SEBN		 单株角果数
NPP		 总根表面积
TRS		 总根体积
TRV
FT 0.807 0.531 0.375 0.357 0.572 0.333 0.386 0.495 0.364 0.483
FS 0.735 0.584 0.468 0.439 0.625 0.421 0.425 0.478 0.477 0.526
PT 0.875 0.598 0.548 0.528 0.597 0.629 0.517 0.765 0.500 0.627
PS 0.875 0.852 0.981 1.000 0.769 1.000 0.979 0.652 0.735 0.870
NT 0.847 0.657 0.597 0.608 0.816 0.629 0.683 1.000 0.544 0.718
NS 1.000 1.000 1.000 0.959 1.000 0.884 1.000 0.762 1.000 0.957
关联度DA (γ) 0.856 0.704 0.662 0.649 0.730 0.649 0.665 0.692 0.603
权重系数WC (ω) 0.138 0.113 0.107 0.104 0.118 0.105 0.107 0.111 0.097
[1] 中华人民共和国国家统计局. 中国统计年鉴. 北京: 中国统计出版社, 2016
National Bureau of Statistics of the People’s Republic of China. China Statistical Yearbook. Beijing: China Statistics Press, 2016 (in Chinese)
[2] 赵小蓉, 蒲波, 李浩, 王昌桃, 赵燮京. 秸秆覆盖栽培对油菜土壤含水率和产量的影响. 西南农业学报, 2010, 23: 354-358
Zhao X R, Pu B, Li H, Wang C T, Zhao X J.Effect of straw covering farming on oilseed rape yield and soil water content under rice-oilseed rape cropping system.Southwest China J Agric Sci, 2010, 23: 354-358 (in Chinese with English abstract)
[3] 薛兰兰, Shakeel A A, 刘晓建, 邹聪明, 胡小东, 张云兰, 王龙昌. 秸秆覆盖对土壤养分和油菜生长发育的影响. 农机化研究, 2011, (2): 110-115
Xue L L, Shakeel A A, Liu X J, Zou C M, Hu X D, Zhang Y L, Wang L C.Effect of straw mulch conservative cultivation on growth, yield and soil nutrients of rapeseed (Brassica compestris). J Agric Mechanization Res, 2011, (2): 110-115 (in Chinese with English abstract)
[4] 蒋声和. 开辟油源的良好途径——绿肥田间作油菜. 农业科学通讯, 1955, (10): 567-568
Jiang S H.A good way of opening the oil resources—green manure intercropping with rape.Agric Sci Commun, 1955, (10): 567-568 (in Chinese)
[5] 江西省农业科学院. 油菜、紫云英混播试验初步报告. 湖北农业科学, 1957, (5): 310-312
Jiangxi Academy of Agricultural Sciences. A preliminary test report of rape mixed with Chinese milk vetch.Hubei Agric Sci, 1957, (5): 310-312 (in Chinese)
[6] 李中栋. 江西省油菜与紫云英间作、混作的经验. 中国农业科学, 1957, (7): 382-384
Li Z D.The experience of rape intercropping with Chinese milk vetch in Jiangxi province.Sci Agric Sin, 1957, (7): 382-384 (in Chinese)
[7] 耿成杰. 湖北省油料作物学会举行油菜豆科绿肥混种间作学术讨论会. 土壤通报, 1963, (6): 59
Geng C J.A seminar of rape intercropping with leguminous green manure—The oil crop science society of Hubei province.Chin J Soil Sci, 1963, (6): 59 (in Chinese)
[8] 周可金, 邢君, 博毓红, 桑亚松, 吴社兰, 宋国良. 油菜与紫云英间混作系统的生理生态效应. 应用生态学报, 2005, 16(8): 1477-1481
Zhou K J, Xing J, Bo Y H, Sang Y S, Wu S L, Song G L.Physiological and ecological effects of inter- and mixed cropping rape with milk vetch.Chin J Appl Ecol, 2005, 16: 1477-1481 (in Chinese with English abstract)
[9] 吴社兰, 周可金. 油菜与紫云英混作系统的密度效应研究. 作物杂志, 2008, (2): 57-59
Wu S L, Zhou K J.Effect of plant density of mixcropping system between rape and milk vetch.Crops, 2008, (2): 57-59 (in Chinese with English abstract)
[10] 宋莉, 韩上, 席莹莹, 鲁剑巍, 吴礼树, 曹卫东, 耿明健. 间作对油菜和紫云英生长及产量的影响. 中国油料作物学报, 2014, 36: 231-237
Song L, Han S, Xi Y Y, Lu J W, Wu L S, Cao W D, Geng M J.Effects of intercropping on growth and yield of rape and Chinese milk vetch.Chin J Oil Crop Sci, 2014, 36: 231-237 (in Chinese with English abstract)
[11] 曹卫东, 黄鸿翔. 关于我国恢复和发展绿肥若干问题的思考. 中国土壤与肥料, 2009, (4): 1-3
Cao W D, Huang H X.Ideas on restoration and development of green manures in China.Chin Soil Fert, 2009, (4): 1-3 (in Chinese with English abstract)
[12] Sang Y K, Chang H L, Jessie G, Pil J K.Contribution of winter cover crop amendments on global warming potential in rice paddy soil during cultivation.Plant Soil, 2013, 366: 273-286
[13] 周泉, 王龙昌, 熊瑛, 张赛, 杜娟, 赵琳璐. 绿肥间作和秸秆覆盖对冬季油菜根际土壤有机碳及土壤呼吸的影响. 环境科学, 2016, 37: 1114-1120
Zhou Q, Wang L C, Xiong Y, Zhang S, Du J, Zhao L L.Effects of green manure intercropping and straw mulching on winter rape rhizosphere soil organic carbon and soil respiration.Environ Sci, 2016, 37: 1114-1120 (in Chinese with English abstract)
[14] Jeromela A M, Mikić A M, Vujić S, Ćupina B, Krstić D, Dimitrijević A, Vasiljević S, Mihailović V, Cvejić S, Miladinović D.Potential of Legume-Brassica intercrops for forage production and green manure: encouragements from a temperate southeast European environment.Front Plant Sci, 2017, 8: 312
[15] Banik P, Sasmal T, Ghosal P K, Bagchi D K.Evaluation of mustard (Brassica compestris var. Toria) and legume intercropping under 1:1 and 2:1 row replacement series systems. J Agron Crop Sci, 2000, 185: 9-14
[16] Devi K N, Shamurailatpam D, Singh T B, Athokpam H S, Singh N B, Singh N G, Singh L N, Singh A D, Chanu O P, Singh S R, Devi K P, Devi L S.Performance of lentil (Lens culinaris M.) and mustard(Brassica juncea L.) intercropping under rainfed conditions. Aust J Crop Sci, 2014, 8: 284-289
[17] 肖靖秀, 汤利, 郑毅. 氮肥用量对油菜蚕豆间作系统作物产量及养分吸收的影响. 植物营养与肥料学报, 2011, 17: 1468-1473
Xiao J X, Tang L, Zheng Y.Effects of N fertilization on yield and nutrient absorption in rape and faba bean intercropping system.Plant Nutr Fert Sci, 2011, 17: 1468-1473 (in Chinese with English abstract)
[18] Cadoux S, Sauzet G, Valantin-Morison M, Pontet C, Champolivier L, Robert C, Lieven J, Flénet F, Mangenot O, Fauvin P, Landé N.Intercropping frost-sensitive legume crops with winter oilseed rape reduces weed competition, insect damage, and improves nitrogen use efficiency.Oilseeds Fats Crops Lipids, 2015, 22(03): D302
[19] 王秋杰, 曹一平, 张福锁, 王兴仁. 间套作研究中的统计分析方法. 植物营养与肥料学报, 1998, 4: 176-182
Wang Q J, Cao Y P, Zhang F S, Wang X R.The statiatical analysis methods in intercropping researches.Plant Nutr Fert Sci, 1998, 4: 176-182 (in Chinese with English abstract)
[20] 廖桂平, 官春云. 不同播期对不同基因型油菜产量特性的影响. 应用生态学报, 2001, 12: 853-858
Liao G P, Guan C Y.Effect of seeding date on yield characteristics of different rapeseed (Brassica napus) genotypes. Chin J Appl Ecol, 2001, 12: 853-858 (in Chinese with English abstract)
[21] 赵秉强, 张福锁, 李增嘉. 间套作条件下作物根系数量与活性的空间分布及变化规律研究: II. 间作早春玉米根系数量与活性的空间分布及变化规律. 作物学报, 2001, 27: 974-980
Zhao B Q, Zhang F S, Li Z J.Vertical distribution and its change of root quantity & activity of crops in the “winter wheat early spring maize/summer maize” cropping system: II. The vertical distribution and its changes of root quantity & activity of the early spring inter-plant.Acta Agron Sin, 2001, 27: 974-980 (in Chinese with English abstract)
[22] Coppens F, Garnier P, Findeling A, Merckx R, Recous S.Decomposition of mulched versus incorporated crop residues: modelling with PASTIS clarifies interactions between residue quality and location.Soil Biol Biochem, 2007, 39: 2339-2350
[23] Zhang X Q, Huang G Q, Bian X M, Zhao Q G.Effects of nitrogen fertilization and root interaction on the agronomic traits of intercropped maize, and the quantity of microorganisms and activity of enzymes in the rhizosphere.Plant & Soil, 2013, 368: 407-417
[24] 赵聚宝, 梅旭荣, 薛军红, 钟兆站, 张天佑. 秸秆覆盖对旱地作物水分利用效率的影响. 中国农业科学, 1996, 29(2): 59-66
Zhao J B, Mei X R, Xue J H, Zhong Z Z, Zhang T Y.The effect of straw mulch on crop water use efficiency in dryland.Sci Agric Sin, 1996, 29(2): 59-66 (in Chinese with English abstract)
[25] 李静静, 李从锋, 李连禄, 丁在松, 赵明. 苗带深松条件下秸秆覆盖对春玉米土壤水温及产量的影响. 作物学报, 2014, 40: 1787-1796
Li J J, Li C F, Li L L, Ding Z S, Zhao M.Effect of straw mulching on soil temperature, soil moisture and spring maize yield under seedling strip subsoiling.Acta Agron Sin, 2014, 40: 1787-1796 (in Chinese with English abstract)
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