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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (01): 58-66.doi: 10.3724/SP.J.1006.2011.00058

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of Sequence Polymorphism of ZmRCA1 in Maize

TAN Xian-Jie1,2,SONG Yan-Chun3,SHI Yun-Su3,CHENG Wei-Dong1,WU Zhi-Kai1,*,WANG Tian-Yu3,LI Yu3,*   

  1. 1 School of Agronomy, Guangxi University, Nanning 530005, China;2 Maize Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530227, China;3Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2010-05-25 Revised:2010-08-04 Online:2011-01-12 Published:2010-11-16

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Abstract: Rubisco is a pivotal enzyme that initiates the first step of carbon fixation and photorespiration in plant photosynthesis. Rubisco accounts for up to half of the soluble protein in the leaves of plants. Nevertheless the catalytic rate of Rubisco iscomparatively low. Rubisco activity is regulated mainly by Rubisco activase, which serves as a molecular chaperone. By activating and regulating Rubisco, RCA potentially influences the efficiency of carbon assimilation in plants. Thereby, RCA has identified a possible target gene for improve production in crops breeding.To investigate polymorphism of ZmRCA1, sequened and analyzed the genomic sequences of ZmRCA1 from a minicore set of 95 maize inbreds lines. Totally 22 SNPs and 8 InDels were identified in a 1 680 bp sequence alignment. There were five SNPs and one InDel which generated amino acid sequence variation. A total of 27 haplotypes were identified with 13 polymorphic loci which the frequency was 0.1 or more. Approximately 90% haplotypes could be distinctly distinguished by six polymorphic loci. The ZmRCA1 gene was highly conserved, with the genic similarity of 97.9% and the amino acid sequence similarity of 99.8%. Neutrality tests showed that no purifying selection occurred in ZmRCA1.

Key words: Rubisco activase (RCA), SNP, InDel, LD, Haplotype, htSNP

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