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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (05): 793-802.doi: 10.3724/SP.J.1006.2011.00793

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

Root Infection and Systematic Colonization of DsRed-labelled Fusarium verticillioides in Maize

WU Lei1,2,WANG Xiao-Ming2,*,XU Rong-Qi3,LI Hong-Jie2,*   

  1. 1 College of Life Science and Technology, Hebei Normal University of Science & Technology, Changli 066600, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Beijing 100081, China; 3 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2010-10-20 Revised:2011-03-08 Online:2011-05-12 Published:2011-03-24
  • Contact: 李洪杰, hongjie@caas.net.cn; 王晓鸣, E-mail: wangxm@mail.caas.net.cn

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Abstract: Fusarium verticillioides (Sacc.) Nirenberg [syn. F. moniliforme J. Sheld., teleomorph: Gibberella fujikuroi (Sawada) Ito in Ito & K. Kimura] is an important causal agent of diseases in maize (Zea mays L.) worldwide, which invades maize plants at all growth stages. This filamentous fungus not only incites obvious symptoms on maize ear, stem, root, and seedling, but also is able to infect plants without any visible symptom (endophytic infection). This makes difficult to detect interaction between this fungus and its hosts.Fluorescent reporter gene-labelled filamentous fungus permits in vitro and in planta monitoring growth and development of the fungus. In this study, gene DsRed encoding red fluorescent protein was delivered into F. verticillioides strain Fv-1 via Agrobacterium tumefaciens-mediated transformation. Using the DsRed-tagged F. verticillioides, systemic colonization of the fungus in roots and stems of the susceptible maize inbred line B73 was investigated. The aim of this study was to understand the interaction between F. verticillioides and maize. The fungus invaded and multiplied inside the lateral root tissues. Some conidia tended to colonize on the veins of the maize root surface and grew along the veins after their germinaiton. Others penetrated the plant cells where they attached and formed hyphae for attacking other cells nearby. Usually, the mycelia migrated from root to stem through intercellular parts of tissues, while some mycelia run across different host cells. Analysis of colony forming unit (CFU) from diseased plants demonstrated that following inoculation the CFU values from roots tended to decrease, but those from stems inclined to increase. This indicates that F. verticillioides is able to attack the aboveground parts of plant via systemic colonization on roots. The results from the present study are useful in disclosing the interaction between F. verticillioides and maize, and can be extended to studying interactions between other soil-borne fungi and plants.

Key words: Maize, Fusarium verticillioides, DsRed, Interaction, Systemic colonization, CFU (colony forming unit)

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