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Acta Agron Sin ›› 2011, Vol. 37 ›› Issue (06): 982-990.doi: 10.3724/SP.J.1006.2011.00982

• CROP GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS • Previous Articles     Next Articles

GmAREB Gene Improves Tolerances to Drought and Oxidation in Transgenic Arabidopsis

GAO Shi-Qing1,CHEN Ming2,XU Zhao-Shi2,TANG Yi-Miao1,LI Lian-Cheng2,MA You-Zhi2,ZHAO Chang-Ping1   

  1. 1 Beijing Engineering and Technical Research Center for Hybrid Wheat / Beijing Academy of Agricultural and Forestry Science, Beijing 100097, China; 2 National Key Facility for Crop Genetic Resources and Genetic Improvement / Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2010-11-12 Revised:2011-03-08 Online:2011-06-12 Published:2011-04-12
  • Contact: 赵昌平, E-mail: cp_zhao@sohu.com; 马有志, E-mail: mayzh@yahoo.com.cn

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Abstract: Abiotic stresses such as drought, high-salt and low temperature severely affect the yield and quality of crops. It is reported that subfamily A of alkalescence leucine zipper transcription factors (basic leucine zipper, bZIP) mainly participated in response to ABA, drought, high salt and oxidative stresses, plays vital roles in stress signal transduction, downstream gene expression regulation and improvement of anti-adversity in plants. In this study, soybean (Glycine max) cv. Tiefeng 8 with salt tolerance was used to isolate and obtain a bZIP transcription factor gene by electronic assembly methods, which was named as GmAREB (Glycine max ABA responsive element binding protein). This gene was composed of 1,317 nucleotides and encoded 439 amino acids. Sequence analysis showed that there were four putative phosphorylation sites (C1, C2, C3, C4), a nuclear localization signal region (KVVE) and a conservative bZIP domain. Homology and phylogenetic trees displayed that GmAREB had the higher homology and more close relationships with Arabidopsis ABF2 and rice TRAB1. To study the combining characteristics of transcription factors and DNA, we carried out electrophoretic mobility shift assay (EMSA) experiment and the results displayed thatGmAREB could specifically bind the ABRE cis-element in vivo. Functional identification of stress treatments showed that the survival rate of 35S::GmAREB transgenic plants treated by drought was higher (50%) than that (5%) of wild type. Stomatal observation displayed that the stomatal aperture of transgenic Arabidopsis (0.8 μm) was less than that of control (2.6 μm) under the drought stress for 2 h. The 35S::GmAREB transgenic plants were treated by methyl viologen (MV) to identify the oxidative stress tolerance of the GmAREB gene. Statistical analysis showed that transgenic Arabidopsis maintained the higher chlorophyl content (7.3 mg g–1 FW) than that of the wild-type. RT-PCR analysis of transgenic Arabidopsis showed that GmAREB overexpression enhanced the expression of downstream stress-related target genes (ABI1, ABI2) and suppressed the stomatal opening-related target genes (KAT1, KAT2). Therefore, the results above illuminated that GmAREB overexpression effectively regulated the expression of downstream target genes, accelerated the stomatal closure, reduced the moisture to evaporate, decreased chlorophyll degradation and enhanced the tolerance to drought and oxidative stresses in transgenic Arabidopsis.

Key words: ABA, bZIP transcription factor, Arabidopsis, Stress tolerance

[1]Liu Q(刘强), Zhao N-M(赵南明), Yamaguchi-Shinozaki K, Shinozaki K. DREB transcription factors to improve plant resistance in the role. Sci Bull (科学通报), 2000, 45(1): 11–16 (in Chinese)
[2]Yamaguchi-Shinozaki K, Kasuga M, Liu Q, Nakashima K, Sakuma Y, Abe H, Shinwari Z K, Seki M, Shinozaki K. Biological mechanisms of drought stress response. JIRCAS Work Rep, 2002, 9: 1–8
[3]Finkelstein R R, Lynch T J. The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor. Plant Cell, 2000, 12: 599–609
[4]Finkelstein R R, Gampala S S, Rock C D. Abscisic acid signaling in seeds and seedlings. Plant Cell, 2002, 14(suppl): 15–45
[5]Jakoby M, Weisshaar B, Droge-Laser W, Vicente-Carbajosa J, Tiedemann J, Kroj T, Parcy F. bZIP transcription factors in Arabidopsis. Trends Plant Sci, 2002, 7: 106–111
[6]Foster R, Izawa T, Chua N H. Plant bZIP proteins gather at ACGT elements. FASEB J, 1994, 8: 192–200
[7]Bray E A. Plant responses to water deficit. Trends Plant Sci, 1997, 2: 48–54
[8]Kang J, Choi H, Kim S Y. Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. Plant Cell, 2002, 14: 343–357
[9]Kim J B, Kang J Y, Kim S Y. Over-expression of a transcription factor regulating ABA-responsive gene expression confers multiple stress tolerance. Plant Biotechnol J, 2004, 2: 459–466
[10]Guiltinan M J, Marcotte W R Jr, Quatrano R S. A plant leucine zipper protein that recognizes an abscisic and response element. Science, 1990, 250: 267–270
[11]Hobo T, Asada M, Kowyama Y, Hattori T. A bZIP factor, TRAB1, interacts with VP1 and mediates abscisic acid-induced transcription. Proc Natl Acad Sci USA, 1999, 26: 15348–15353
[12]Choi H, Hong J, Kang J, Kim S Y. ABFs, a family of ABA-responsive element binding factors. J Biol Chem, 2000, 21: 1723–1730
[13]Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez Mohammad M, Seki M, Keiichiro H, Masaru O T, Shinozaki K, Yamaguchi-Shinozaki K. AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis. Plant Cell, 2005, 17: 3470–3488
[14]Oeda K, Salinas J, Chua N H. A tobacco bZIP transcription activator (TAF-1) binds to G-BOX-like motif conserved in plant genes. EMBO J, 1991, 10: 1793–1802
[15]Kagaya Y, Hobo T, Murata M, Ban A, Hattori T. Abscisic acid–induced transcription is mediated by phosphorylation of an abscisic acid response element binding factor, TRAB1. Plant Cell, 2002, 14: 3177–3189
[16]Xiang Y, Tang N, Du H, Ye H Y, Xiong L Z H. Characterization of OsbZIP23 as a key player of the basic leucine zipper transcription factor family for conferring abscisic acid sensitivity and salinity and drought tolerance in rice. Plant Physiol, 2008, 148: 1938–1952
[17]Lu G J, Gao C X, Zheng X N, Han B. Identification of OsbZIP72 as a positive regulator of ABA response and drought tolerance in rice. Planta, 2009, 229: 605–615
[18]Jin X F, Xiong A S, Peng R H, Liu J G, Gao F, Chen J M, Yao Q H. OsAREB1, an ABRE-binding protein responding to ABA and glucose, has multiple functions in Arabidopsis. Biochem Mol Biol Rep, 2010, 43: 34–39
[19]Liao Y, Zou H F, Wei W, Hao Y J, Tian A G, Huang J, Liu Y F, Zhang J S, Chen S Y. Soybean GmbZIP44, GmbZIP62 and GmbZIP78 genes function as negative regulator of ABA signaling and confer salt and freezing tolerance in transgenic Arabidopsis. Planta, 2008, 228: 225–240
[20]Liao Y, Zhang J S, Chen S Y, Zhang W K. Role of soybean GmbZIP132 under abscisic acid and salt stresses. J Integr Plant Biol, 2008, 50: 221–230
[21]Wang L(王磊), Zhao J(赵军), Fan Y-L(范云六). Corn Cat1 gene the cis-regulatory elements ABRE2 binding protein ABP9 gene clone and functional analysis. Sci Bull (科学通报), 2002, 15: 1167–1171 (in Chinese)
[22]Casaretto J, Ho T H. The transcription factors HvABI5 and HvVP1 are require for the abscisic acid induction of gene expression in barley Aleurone cells. Plant Cell, 2003, 15: 271–284
[23]Hsieh T H, Li C W, Su R C, Cheng C P, Sanjaya, Tsai Y C, Chan M T. A tomato bZIP transcription factor, SlAREB, is involved in water deficit and salt stress response. Planta, 2010, 231: 1459–1473
[24]Satoh R, Fujita Y, Nakashima K, Shinozaki K, Yamaguchi-Shinozaki K. A novel subgroup of bZIP proteins functions as transcriptional activators in hypoosmolarity-responsive expression of the ProDH gene in Arabidopsis. Plant Cell Physiol, 2004, 45: 309–317
[25]Uno Y, Furihata T, Abe H, Yoshida R, Shinozaki K, Yamaguchi-Shinozaki K. Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. Proc Natl Acad Sci USA, 2000, 97: 11632–11637
[26]Kim S, Kang J Y, Cho D I, Park J H, Kim S Y. ABF2, an ABRE-binding bZIP factor, is an essential component of glucose signaling and its overexpression affects multiple stress tolerance. Plant J, 2004, 40: 75–87
[27]Furihata T, Maruyama K, Fujita Y, Umezawa T, Yoshida R, Shinozaki K, Yamaguchi-Shinozaki K. Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1. Proc Natl Acad Sci USA, 2006, 103: 1988–1993
[28]Yoshida T, Fujita Y, Sayama H, Kidokoro S, Maruyama K, Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K. AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation. Plant J, 2010, 61: 672–685
[29]Maxwell D P, Wang Y, McIntosh L. The alternative oxidase lowers mitochondrial reactive oxygen production in plant cells. Proc Natl Acad Sci USA, 1999, 96: 8271–8276
[30]Mittler R, Vanderauwera S, Gollery M, Van Breusegem F. Reactive oxygen gene network of plants. Trends Plant Sci, 2004, 9: 490–498
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